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SY SY - Synaptic Systems SY SY - Synaptic Systems

SNAP 25 - 111 011

Palmitoylated plasma membrane protein; required for exocytosis

Monoclonal mouse purified IgG
415.00 USD
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Cat. No. 111 011 50 µg purified IgG, lyophilized. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 10000 (AP staining) blot  
IP: yes (see remarks)
ICC: 1 : 100 up to 1 : 1000 image  
IHC: not recommended
IHC-P/FFPE: 1 : 2000 image  
EM: yes
Clone 71.1
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 206 from rat SNAP25B (UniProt Id: P60881-1)
Epitop Epitop: AA 20 to 40 from rat SNAP25B (UniProt Id: P60881-1)
Reactivity Reacts with: human (P60880), rat (P60881), mouse (P60879), vertebrates, invertebrates.
Other species not tested yet.
Specificity Specific for SNAP 25.
Remarks

IP: Immunoprecipitation not quantitative, appears to depend on the binding status of the protein.
Recognizes the Botulinum neurotoxin A and E cleavage products.
Recognizes splice variants SNAP 25A and B.

Datasheet 111_011.pdf


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References for SNAP 25 - 111 011

Synaptotagmin oligomerization is essential for calcium control of regulated exocytosis.
Bello OD, Jouannot O, Chaudhuri A, Stroeva E, Coleman J, Volynski KE, Rothman JE, Krishnakumar SS
Proceedings of the National Academy of Sciences of the United States of America (2018) 11532: E7624-E7631. 111 011 WB; tested species: rat
CRISPR interference-based specific and efficient gene inactivation in the brain.
Zheng Y, Shen W, Zhang J, Yang B, Liu YN, Qi H, Yu X, Lu SY, Chen Y, Xu YZ, Li Y, et al.
Nature neuroscience (2018) 213: 447-454. 111 011 WB; KD verified; tested species: mouse
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 111 011 WB
MiR-335 overexpression impairs insulin secretion through defective priming of insulin vesicles.
Salunkhe VA, Ofori JK, Gandasi NR, Salö SA, Hansson S, Andersson ME, Wendt A, Barg S, Esguerra JLS, Eliasson L
Physiological reports (2017) 521: . 111 011 WB; tested species: rat
Plekhg5-regulated autophagy of synaptic vesicles reveals a pathogenic mechanism in motoneuron disease.
Lüningschrör P, Binotti B, Dombert B, Heimann P, Perez-Lara A, Slotta C, Thau-Habermann N, R von Collenberg C, Karl F, Damme M, Horowitz A, et al.
Nature communications (2017) 81: 678. 111 011 WB
Olanzapine Reverses MK-801-Induced Cognitive Deficits and Region-Specific Alterations of NMDA Receptor Subunits.
Liu X, Li J, Guo C, Wang H, Sun Y, Wang H, Su YA, Li K, Si T
Frontiers in behavioral neuroscience (2017) 11: 260. 111 011 WB; tested species: rat
How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 111 011 WB
Vesicular Synaptobrevin/VAMP2 Levels Guarded by AP180 Control Efficient Neurotransmission.
Koo SJ, Kochlamazashvili G, Rost B, Puchkov D, Gimber N, Lehmann M, Tadeus G, Schmoranzer J, Rosenmund C, Haucke V, Maritzen T, et al.
Neuron (2015) 882: 330-44. 111 011 WB; tested species: mouse
Ubiquitin-Synaptobrevin Fusion Protein Causes Degeneration of Presynaptic Motor Terminals in Mice.
Liu Y, Li H, Sugiura Y, Han W, Gallardo G, Khvotchev M, Zhang Y, Kavalali ET, Südhof TC, Lin W
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3533: 11514-31. 111 011 WB
Wnt signalling tunes neurotransmitter release by directly targeting Synaptotagmin-1.
Ciani L, Marzo A, Boyle K, Stamatakou E, Lopes DM, Anane D, McLeod F, Rosso SB, Gibb A, Salinas PC
Nature communications (2015) 6: 8302. 111 011 WB
Synaptotagmin-1 and -7 Are Redundantly Essential for Maintaining the Capacity of the Readily-Releasable Pool of Synaptic Vesicles.
Bacaj T, Wu D, Burré J, Malenka RC, Liu X, Südhof TC
PLoS biology (2015) 1310: e1002267. 111 011 WB
Complexin stabilizes newly primed synaptic vesicles and prevents their premature fusion at the mouse calyx of held synapse.
Chang S, Reim K, Pedersen M, Neher E, Brose N, Taschenberger H
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3521: 8272-90. 111 011 WB
α-Synuclein assembles into higher-order multimers upon membrane binding to promote SNARE complex formation.
Burré J, Sharma M, Südhof TC
Proceedings of the National Academy of Sciences of the United States of America (2014) 11140: E4274-83. 111 011 WB
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 111 011 WB, ICC; tested species: rat
Phosphorylation of syntaxin 3B by CaMKII regulates the formation of t-SNARE complexes.
Liu X, Heidelberger R, Janz R
Molecular and cellular neurosciences (2014) 60: 53-62. 111 011 WB; tested species: mouse
C-terminal complexin sequence is selectively required for clamping and priming but not for Ca2+ triggering of synaptic exocytosis.
Kaeser-Woo YJ, Yang X, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 328: 2877-85. 111 011 WB; tested species: rat
CSPα promotes SNARE-complex assembly by chaperoning SNAP-25 during synaptic activity.
Sharma M, Burré J, Südhof TC
Nature cell biology (2011) 131: 30-9. 111 011 WB; tested species: mouse
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 111 011 WB, ICC
SV2 mediates entry of tetanus neurotoxin into central neurons.
Yeh FL, Dong M, Yao J, Tepp WH, Lin G, Johnson EA, Chapman ER
PLoS pathogens (2010) 611: e1001207. 111 011 WB; tested species: mouse
SV2B regulates synaptotagmin 1 by direct interaction.
Lazzell DR, Belizaire R, Thakur P, Sherry DM, Janz R
The Journal of biological chemistry (2004) 27950: 52124-31. 111 011 WB; tested species: mouse
Regulation of releasable vesicle pool sizes by protein kinase A-dependent phosphorylation of SNAP-25.
Nagy G, Reim K, Matti U, Brose N, Binz T, Rettig J, Neher E, Sørensen JB
Neuron (2004) 413: 417-29. 111 011 WB
An essential role for vesicular glutamate transporter 1 (VGLUT1) in postnatal development and control of quantal size.
Wojcik SM, Rhee JS, Herzog E, Sigler A, Jahn R, Takamori S, Brose N, Rosenmund C
Proceedings of the National Academy of Sciences of the United States of America (2004) 10118: 7158-63. 111 011 WB; tested species: mouse
Riluzole attenuates the efficacy of glutamatergic transmission by interfering with the size of the readily releasable neurotransmitter pool.
Lazarevic V, Yang Y, Ivanova D, Fejtova A, Svenningsson P
Neuropharmacology (2018) : . 111 011 ICC; tested species: rat
Atoxic derivative of botulinum neurotoxin A as a prototype molecular vehicle for targeted delivery to the neuronal cytoplasm.
Vazquez-Cintron EJ, Vakulenko M, Band PA, Stanker LH, Johnson EA, Ichtchenko K
PloS one (2014) 91: e85517. 111 011 ICC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 111 011 WB, ICC; tested species: rat
Synaptotagmin interaction with SNAP-25 governs vesicle docking, priming, and fusion triggering.
Mohrmann R, de Wit H, Connell E, Pinheiro PS, Leese C, Bruns D, Davletov B, Verhage M, Sørensen JB
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3336: 14417-30. 111 011 ICC
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 111 011 WB, ICC
Temporal characteristics of vesicular fusion in astrocytes: examination of synaptobrevin 2-laden vesicles at single vesicle resolution.
Malarkey EB, Parpura V
The Journal of physiology (2011) 58917: 4271-300. 111 011 ICC; tested species: rat
Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells.
Kirizs T, Kerti-Szigeti K, Lorincz A, Nusser Z
The European journal of neuroscience (2014) 3911: 1771-83. 111 011 EM; tested species: rat
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 111 011
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com