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Synaptotagmin4 protein - 105-4P

Synaptotagmin4 is located at the Golgi compartment and LDCVs in NGF differentiated PC12 cells
control protein
Cat. No.: 105-4P
Amount: 100 µg
Price: $105.00
Cat. No. 105-4P 100 µg protein, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Control proteins should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
Immunogen Recombinant protein corresponding to AA 40 to 151 from rat Synaptotagmin4 (UniProt Id: P50232)
Recommended dilution Optimal concentrations should be determined by the end-user.
Matching antibodies 105 043, 105 143
Remarks

This control protein consists of the recombinant protein (aa 40-151 of rat synaptotagmin 4) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of protein needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Data sheet 105-4p.pdf
Cat. No.: 105-4P
Amount: 100 µg
Price: $105.00
Background
Up to now at least 17 synaptotagmins have been identified. Synaptotagmin 4 is composed of a vesicular, a transmembrane and two C2 domains. Only the C2B domain is able to bind calcium. In the C2A domain one of the calcium binding aspartates has been substituted for serine leading to a loss of its binding capabilities.
The localization of synaptotagmin 4 is still under discussion. A localization to synaptic vesicles (SVs) has been postulated but more recent studies suggest that it is present in the Golgi compartment, in distal parts of neurites and on large dense core vesicles (LDCVs) of NGF differentiated PC12 cells.
Protocols