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Home > Products > 105 223CpH

Synaptotagmin2 antibody luminal domain - 105 223CpH

Synaptotagmin2 is an alternative Ca2+-sensor for neurotransmitter release
Rabbit polyclonal purified antibody
Cat. No.: 105 223CpH
Amount: 50 µg
Price: $480.00
Cat. No. 105 223CpH 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with CypHer5E.

CypHer5E® is a pH-sensitive dye, fluorescent only after antibody internalization at the acidic pH of the lumen of the synaptic vesicle. CypHer5E® can be detected with the filter sets for Cy5®.

CypHer5E®: λex 647 nm / λem 667 nm

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Application Number US 2006-0051758 and related patents. Cy, CyDye, and CypHer are trademarks of GE-Healthcare Ltd. Purchase of reagents related to the CypHer technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from GE-Healthcare Bio-Science Corp., 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.

 Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 100 gallery  
IHC: not tested yet
IHC-P: not tested yet
Label CypHer5E
Immunogen Synthetic peptide corresponding to AA 1 to 11 from mouse Synaptotagmin2 (UniProt Id: P46097)
Reactivity Reacts with: rat (P29101), mouse (P46097).
Other species not tested yet.
Remarks

This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.
Data sheet 105_223cph.pdf
Cat. No.: 105 223CpH
Amount: 50 µg
Price: $480.00
ICC
Background

Synaptotagmin 2 is an integral membrane glycoprotein of neuronal synaptic vesicles. It is very similar to synaptotagmin 1 but shows a partly complementary expression pattern in the CNS. Synaptotagmin 2 lacks a CAMK II/PKC phosphorylation site which is present in synaptotagmin 1.
Recently synaptotagmin 2 has been shown to be an alternative Ca2+ sensor for fast secretion.
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