Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 105 311BT

Synaptotagmin1 antibody luminal domain - 105 311BT

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release
Mouse monoclonal purified IgG
Cat. No.: 105 311BT
Amount: 100 µg
Price: $465.00
Cat. No. 105 311BT 100 µg purified IgG, lyophilized, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

 For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 gallery  
IP: yes
ICC: 1 : 50 up to 1 : 300 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
Label biotin
Clone 604.2
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 1 to 12 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: rat (P21707).
No signal: mouse (P46096), zebrafish.
Other species not tested yet.
Remarks

ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.
Data sheet 105_311bt.pdf

References for Synaptotagmin1 - 105 311BT

Unique dynamics and exocytosis properties of GABAergic synaptic vesicles revealed by three-dimensional single vesicle tracking.
Park C, Chen X, Tian CL, Park GN, Chenouard N, Lee H, Yeo XY, Jung S, Tsien RW, Bi GQ, Park H, et al.
Proceedings of the National Academy of Sciences of the United States of America (2021) 1189: . 105 311BT UPTAKE; tested species: rat
Real-time three-dimensional tracking of single synaptic vesicles reveals that synaptic vesicles undergoing kiss-and-run fusion remain close to their original fusion site before reuse.
Qin X, Tsien RW, Park H
Biochemical and biophysical research communications (2019) : . 105 311BT UPTAKE; tested species: rat
Cat. No.: 105 311BT
Amount: 100 µg
Price: $465.00
Unique dynamics and exocytosis properties of GABAergic synaptic vesicles revealed by three-dimensional single vesicle tracking.
Park C, Chen X, Tian CL, Park GN, Chenouard N, Lee H, Yeo XY, Jung S, Tsien RW, Bi GQ, Park H, et al.
Proceedings of the National Academy of Sciences of the United States of America (2021) 1189: . 105 311BT UPTAKE; tested species: rat
Real-time three-dimensional tracking of single synaptic vesicles reveals that synaptic vesicles undergoing kiss-and-run fusion remain close to their original fusion site before reuse.
Qin X, Tsien RW, Park H
Biochemical and biophysical research communications (2019) : . 105 311BT UPTAKE; tested species: rat
WB
ICC
Background
Synaptotagmin 1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.
Other products for this target