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|Cat. No. 105 311AF||
100 µg purified IgG, lyophilized, fluorescence-labeled with AcidiFluor Orange . Rabbit serum albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
ICC: 1 : 100 up to 1 : 500 gallery
IHC: not tested yet
IHC-P/FFPE: not tested yet
FACS: not tested yet
|Immunogen||Synthetic peptide corresponding to AA 1 to 12 from rat Synaptotagmin1 (UniProt Id: P21707)|
Epitop: AA 1 to 12 from rat Synaptotagmin1 (UniProt Id: P21707)
Reacts with: rat (P21707).
No signal: mouse, zebrafish.
Other species not tested yet.
This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures. The pH sensitive dye regaines its fluorescence after the reacidification of the synaptic vesicle lumen.