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SY SY - Synaptic Systems SY SY - Synaptic Systems

Synaptotagmin 1
lumenal domain - 105 311AF

Ca2+-sensor on synaptic vesicles, triggers neurotransmitter release

pH-sensitive probe fluorescent only under acidic conditions

Monoclonal mouse purified IgG
465.00 USD
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Cat. No. 105 311AF 100 µg purified IgG, lyophilized, fluorescence-labeled with AcidiFluor Orange . Rabbit serum albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
EM: N/A
ELISA: N/A
FACS: not tested yet
Label AcidiFluor Orange
Clone 604.2
Subtype IgG1
Immunogen Synthetic peptide corresponding to AA 1 to 12 from rat Synaptotagmin1 (UniProt Id: P21707)
Epitop Epitop: AA 1 to 12 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: rat (P21707).
No signal: mouse, zebrafish.
Other species not tested yet.
Remarks

This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures. The pH sensitive dye regaines its fluorescence after the reacidification of the synaptic vesicle lumen.

Datasheet 105_311af.pdf


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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com