Cat. No.: 105 103CpH
Amount: 50 µg
Price:
$495.00
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pH-sensitive probe fluorescent only under acidic conditions
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| Cat. No. 105 103CpH |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with CypHer5E. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications |
IP: N/A ICC: 1 : 100 up to 1 : 500 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet |
| Label | CypHer5E |
| Immunogen | Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096) |
| Reactivity |
Reacts with: rat (P21707), mouse (P46096). Other species not tested yet. For unknown reasons antibodies raised against the luminal N-terminus of Synaptotagmin 1 show a strong preference for the rat protein. |
| Matching control protein/peptide | 105-10P |
| Remarks |
ICC: This antibody can only be used for labeling of recycling synaptic vesicles in living neurons. It is not recommended for the staining of fixed cells. |
| Data sheet | 105_103cph.pdf |
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