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|Cat. No. 105 103AF||
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with AcidiFluor Orange . Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
ICC: 1 : 100 up to 1 : 300 (see remarks) gallery
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Immunogen||Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096)|
Reacts with: rat (P21707).
Other species not tested yet.
|Specificity||specific for Synaptotagmin 1|
|matching control protein/peptide||105-10P|
ICC: This antibody is intended for direct labeling of recycling synaptic vesicles in living primary neuronal cultures. The pH-sensitive dye regaines its fluorescence after the reacidification of the synaptic vesicle lumen.