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SY SY - Synaptic Systems SY SY - Synaptic Systems

Synaptotagmin 1
lumenal domain - 105 103AF

Ca2+-sensor on synaptic vesicles, triggers neurotransmitter release

pH-sensitive probe fluorescent only under acidic conditions

Polyclonal rabbit purified antibody
475.00 USD
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Cat. No. 105 103AF 50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with AcidiFluor Orange . Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 300 (see remarks) gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
Label AcidiFluor Orange
Immunogen Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096)
Reactivity Reacts with: rat (P21707).
Other species not tested yet.
Specificity specific for Synaptotagmin 1
matching control protein/peptide 105-10P
Remarks

ICC: This antibody is intended for direct labeling of recycling synaptic vesicles in living primary neuronal cultures. The pH-sensitive dye regaines its fluorescence after the reacidification of the synaptic vesicle lumen.

Datasheet 105_103af.pdf


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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com