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|Cat. No. 105 008||50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 up to 1 : 5000 (AP staining) (see remarks) gallery
ICC: 1 : 1000 gallery
IHC: 1 : 500 gallery
IHC-P/FFPE: 1 : 100 up to 1 : 1000 gallery
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 80 to 421 from rat Synaptotagmin1 (UniProt Id: P21707)|
Epitop: AA 150 to 240 from rat Synaptotagmin1 (UniProt Id: P21707)
Reacts with: rat (P21707), mouse (P46096), human (P21579), mammals, zebrafish.
Other species not tested yet.
|Specificity||Specific for mammalian synaptotagmin 1, no cross-reactivity to other synaptotagmins. (K.O. verified)|
This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 41.1. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
WB: Non-boiled samples are recommended for SDS-PAGE.