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Synaptotagmin1/2 antibody cytoplasmic tail - 105 003 K.O.

Synaptotagmin1/2 are Ca2+-sensors on synaptic vesicles that trigger neurotransmitter release
Rabbit polyclonal purified antibody
Cat. No.: 105 003
Amount: 50 µg
Price: $455.00
Cat. No. 105 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: not tested yet
ELISA: yes (see remarks)
Immunogen Synthetic peptide corresponding to AA 120 to 131 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: human (P21579), rat (P21707), mouse (P46096), cow, chicken, goldfish, zebrafish.
Other species not tested yet.
Specificity Some cross-reactivity to synaptotagmin 2. K.O. validated PubMed: 32015138
Matching control protein/peptide 105-0P
Remarks

ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 105 011 as capture antibodies. The ELISA-protocol for membrane proteins is recommended.
Data sheet 105_003.pdf

References for Synaptotagmin1/2 - 105 003

Synaptotagmin 1 oligomers clamp and regulate different modes of neurotransmitter release.
Tagliatti E, Bello OD, Mendonça PRF, Kotzadimitriou D, Nicholson E, Coleman J, Timofeeva Y, Rothman JE, Krishnakumar SS, Volynski KE
Proceedings of the National Academy of Sciences of the United States of America (2020) : . 105 003 WB, ICC; KO verified; tested species: mouse
Monocytic Infiltrates Contribute to Autistic-like Behaviors in a Two-Hit Model of Neurodevelopmental Defects.
Chen HR, Chen CW, Mandhani N, Short-Miller JC, Smucker MR, Sun YY, Kuan CY
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4049: 9386-9400. 105 003 WB; tested species: mouse
Hevin-calcyon interaction promotes synaptic reorganization after brain injury.
Kim JH, Jung HG, Kim A, Shim HS, Hyeon SJ, Lee YS, Han J, Jung JH, Lee J, Ryu H, Park JY, et al.
Cell death and differentiation (2021) 289: 2571-2588. 105 003 ICC; tested species: mouse
Synaptotagmin 1 oligomers clamp and regulate different modes of neurotransmitter release.
Tagliatti E, Bello OD, Mendonça PRF, Kotzadimitriou D, Nicholson E, Coleman J, Timofeeva Y, Rothman JE, Krishnakumar SS, Volynski KE
Proceedings of the National Academy of Sciences of the United States of America (2020) : . 105 003 WB, ICC; KO verified; tested species: mouse
Riluzole attenuates the efficacy of glutamatergic transmission by interfering with the size of the readily releasable neurotransmitter pool.
Lazarevic V, Yang Y, Ivanova D, Fejtova A, Svenningsson P
Neuropharmacology (2018) : . 105 003 ICC; tested species: rat
Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 105 003 ICC; tested species: rat
Cell Types Promoting Goosebumps Form a Niche to Regulate Hair Follicle Stem Cells.
Shwartz Y, Gonzalez-Celeiro M, Chen CL, Pasolli HA, Sheu SH, Fan SM, Shamsi F, Assaad S, Lin ET, Zhang B, Tsai PC, et al.
Cell (2020) : . 105 003 IHC; tested species: mouse
Cat. No.: 105 003
Amount: 50 µg
Price: $455.00
Synaptotagmin 1 oligomers clamp and regulate different modes of neurotransmitter release.
Tagliatti E, Bello OD, Mendonça PRF, Kotzadimitriou D, Nicholson E, Coleman J, Timofeeva Y, Rothman JE, Krishnakumar SS, Volynski KE
Proceedings of the National Academy of Sciences of the United States of America (2020) : . 105 003 WB, ICC; KO verified; tested species: mouse
Monocytic Infiltrates Contribute to Autistic-like Behaviors in a Two-Hit Model of Neurodevelopmental Defects.
Chen HR, Chen CW, Mandhani N, Short-Miller JC, Smucker MR, Sun YY, Kuan CY
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4049: 9386-9400. 105 003 WB; tested species: mouse
Hevin-calcyon interaction promotes synaptic reorganization after brain injury.
Kim JH, Jung HG, Kim A, Shim HS, Hyeon SJ, Lee YS, Han J, Jung JH, Lee J, Ryu H, Park JY, et al.
Cell death and differentiation (2021) 289: 2571-2588. 105 003 ICC; tested species: mouse
Synaptotagmin 1 oligomers clamp and regulate different modes of neurotransmitter release.
Tagliatti E, Bello OD, Mendonça PRF, Kotzadimitriou D, Nicholson E, Coleman J, Timofeeva Y, Rothman JE, Krishnakumar SS, Volynski KE
Proceedings of the National Academy of Sciences of the United States of America (2020) : . 105 003 WB, ICC; KO verified; tested species: mouse
Riluzole attenuates the efficacy of glutamatergic transmission by interfering with the size of the readily releasable neurotransmitter pool.
Lazarevic V, Yang Y, Ivanova D, Fejtova A, Svenningsson P
Neuropharmacology (2018) : . 105 003 ICC; tested species: rat
Regulation of density of functional presynaptic terminals by local energy supply.
Zhou H, Liu G
Molecular brain (2015) 8: 42. 105 003 ICC; tested species: rat
Cell Types Promoting Goosebumps Form a Niche to Regulate Hair Follicle Stem Cells.
Shwartz Y, Gonzalez-Celeiro M, Chen CL, Pasolli HA, Sheu SH, Fan SM, Shamsi F, Assaad S, Lin ET, Zhang B, Tsai PC, et al.
Cell (2020) : . 105 003 IHC; tested species: mouse
WB
ICC
IHC
Background
Synaptotagmin 1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.
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