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Home > Products > 101 011BT

Synaptophysin1 antibody - 101 011BT K.O.

Synaptophysin is a major synaptic vesicle membrane protein and a useful neuroendocrine tumor marker
Mouse monoclonal purified IgG
Cat. No.: 101 011BT
Amount: 50 µg
Price: $475.00
Cat. No. 101 011BT 50 µg purified IgG, lyophilized, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

 Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 10000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 up to 1 : 1000 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 100 gallery  
Label biotin
Clone 7.2
Subtype IgG1 (λ light chain)
Immunogen Full-length recombinant rat Synaptophysin (UniProt Id: P07825)
Epitop Epitop: AA 219 to 307 from rat Synaptophysin1 (UniProt Id: P07825)
Reactivity Reacts with: human (P08247), rat (P07825), mouse (Q62277), mammals.
Weaker signal: zebrafish, other vertebrates.
Other species not tested yet.
Specificity K.O. validated
Remarks

Widely used as marker for nerve terminals and neuroendocrine tumors. For unknown reason, neuronal synaptophysin is better recognised than neuroendocrine synaptophysin. If this is a problem, the polyclonal rabbit antibody, cat. no. 101 002 or 101 203 are recommended.
Data sheet 101_011bt.pdf
Cat. No.: 101 011BT
Amount: 50 µg
Price: $475.00
WB
ICC
IHC
IHC-P
Background

Synaptophysin 1, also referred to as p38-1, is a membrane glycoprotein of synaptic vesicles that is ubiquitously expressed in all neurons and in many endocrine cells. It is currently the most widely used marker for nerve terminals and probably the best marker for the pathologist in differentiating neuroendocrine tumors.
Synaptophysin 1 has four transmembrane domains with both N- and C-terminus facing the cytoplasm. It binds to synaptobrevin 1 and synaptobrevin 2 in detergent extracts but its function has not been elucidated completely. It forms a complex with dynamin at high Ca2+ concentration suggesting an involvement in synaptic vesicle endocytosis. As typical for synaptic vesicle proteins, synaptophysin 1 represents a small protein family with two additonal members, synaptoporin (synaptophysin 2) and panthophysin. Like synaptophysin 1, synaptoporin is widely expressed in neurons and colocalizes with synaptophysin 1 on synaptic vesicles whereas panthophysin is expressed in all tissues.
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