Synaptobrevin 2 - 104 318
Major vesicle protein involved in fusion
Recombinant Guinea pig purified IgG
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|Cat. No. 104 318||50 µg purified IgG, lyophilized. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery
IP: yes (see remarks)
ICC: 1 : 500 up to 1 : 1000 gallery
IHC: 1 : 500 up to 1 : 2000 gallery
IHC-P/FFPE: 1 : 400 up to 1 : 1000 gallery
|Subtype||IgG2 (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 2 to 17 from rat Synaptobrevin2 (UniProt Id: P63045)|
Epitop: AA 2 to 17 from rat Synaptobrevin2 (UniProt Id: P63045)
Reacts with: mouse (P63044), rat (P63045), human (P63027), hamster.
No signal: chicken, zebrafish.
Other species not tested yet.
|Specificity||Specific for VAMP 2. No cross-reactivity to VAMP 1 and VAMP 3. (K.O. verified)|
|matching control protein/peptide||104-2P|
This antibody is a chimeric antibody based on the well known monoclonal mouse antibody 69.1. The constant regions of the heavy and light chains have been replaced with Guinea pig specific sequences. The antibody can therefore be used with standard anti-Guinea pig secondary reagents.
IP: This antibody quantitatively precipitates synaptobrevin 2 from detergent extracts regardless of whether the protein is associated.