Synaptic Systems

Cat. No.: 168 103

Amount: 50 µg

Price: $380.00

Cat. No. 168 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 400 gallery IHC-P: 1 : 200 gallery
Immunogen	Recombinant protein corresponding to AA 1 to 218 from human Rab27b (UniProt Id: O00194)
Reactivity	Reacts with: rat (Q99P74), mouse (Q99P58), human (O00194). Other species not tested yet.
Specificity	Specific for rab 27B with weak cross reaction to rab 27A. K.O. validated PubMed: 26845357
Matching control protein/peptide	168-1P
Data sheet	168_103.pdf

zDHHC9 Regulates Cardiomyocyte Rab3a Activity and Atrial Natriuretic Peptide Secretion Through Palmitoylation of Rab3gap1.
Essandoh K, Subramani A, Ferro OA, Teuber JP, Koripella S, Brody MJ
JACC. Basic to translational science (2023) 85: 518-542. 168 103 WB; tested species: mouse

A novel association between platelet filamin A and soluble N-ethylmaleimide sensitive factor attachment proteins regulates granule secretion.
Golla K, Paul M, Lengyell TC, Simpson EM, Falet H, Kim H
Research and practice in thrombosis and haemostasis (2023) 74: 100019. 168 103 WB; tested species: mouse

Actions of Rab27B-GTPase on mammalian central excitatory synaptic transmission.
Arias-Hervert ER, Xu N, Njus M, Murphy GG, Hou Y, Williams JA, Lentz SI, Ernst SA, Stuenkel EL
Physiological reports (2020) 89: e14428. 168 103 WB, IHC; KO verified; tested species: mouse

MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation.
Yoon S, Kovalenko A, Bogdanov K, Wallach D
Immunity (2017) 471: 51-65.e7. 168 103 WB; KD verified; tested species: human

Genetic deletion of Rab27B in pancreatic acinar cells affects granules size and has inhibitory effects on amylase secretion.
Hou Y, Ernst SA, Lentz SI, Williams JA
Biochemical and biophysical research communications (2016) 4714: 610-5. 168 103 WB; KO verified

Rab27A Is Present in Mouse Pancreatic Acinar Cells and Is Required for Digestive Enzyme Secretion.
Hou Y, Ernst SA, Stuenkel EL, Lentz SI, Williams JA
PloS one (2015) 105: e0125596. 168 103 WB, IHC

EPI64B acts as a GTPase-activating protein for Rab27B in pancreatic acinar cells.
Hou Y, Chen X, Tolmachova T, Ernst SA, Williams JA
The Journal of biological chemistry (2013) 28827: 19548-57. 168 103 WB

Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis.
Pavlos NJ, Grønborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO, Jahn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3040: 13441-53. 168 103 WB, ICC

Cat. No.: 168 103

Amount: 50 µg

Price: $380.00

ICC

IHC

IHC-P

Background

Protocols

Rab27B antibody - 168 103 K.O.