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Piccolo antibody - 142 116

Piccolo is a large multi-domain protein of the presynaptic active zone
Chicken polyclonal IgY fraction
Cat. No.: 142 116
Amount: 200 µl
Price: $360.00
Cat. No. 142 116 200 µl antibody, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
ExM: 1 : 100 (see remarks) gallery  
Immunogen Recombinant protein corresponding to a central region of rat piccolo (UniProt Id: Q9JKS6)
Reactivity Reacts with: rat (Q9JKS6), mouse (Q9QYX7).
Other species not tested yet.
Remarks

ExM: This antibody has been successfully used for the magnified analysis of the proteome (MAP) expansion microscopy method (MAP; Ku et al. 2016. Nature Biotechnology 34:973-981)

Data sheet 142_116.pdf

References for Piccolo - 142 116

Synaptic Vesicle Recycling Pathway Determines Neurotransmitter Content and Release Properties.
Silm K, Yang J, Marcott PF, Asensio CS, Eriksen J, Guthrie DA, Newman AH, Ford CP, Edwards RH
Neuron (2019) : . 142 116 ICC; tested species: mouse
Cat. No.: 142 116
Amount: 200 µl
Price: $360.00
Synaptic Vesicle Recycling Pathway Determines Neurotransmitter Content and Release Properties.
Silm K, Yang J, Marcott PF, Asensio CS, Eriksen J, Guthrie DA, Newman AH, Ford CP, Edwards RH
Neuron (2019) : . 142 116 ICC; tested species: mouse
Background

Piccolo, also referred to as Aczonin, is a large protein which constists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains (PBH-domains) and C-terminal PDZ and C2 domains. In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones).
Piccolo is supposed to be a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Recently piccolo has been shown to interfere with clathrin mediated endocytosis by binding to the F-actin and dynamin binding protein Abp1.