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Piccolo antibody - 142 113

Piccolo is a large multi-domain protein of the presynaptic active zone
Rabbit polyclonal purified antibody
Cat. No.: 142 113
Amount: 50 µg
Price: $375.00
Cat. No. 142 113 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not recommended
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
Immunogen Recombinant protein corresponding to a central region of rat piccolo (UniProt Id: Q9JKS6)
Epitop Epitop: AA 2011 to 2350 from rat Piccolo (UniProt Id: Q9JKS6)
Reactivity Reacts with: rat (Q9JKS6), mouse (Q9QYX7).
Other species not tested yet.
Remarks

WB: This antibody is very sensitive and the first choice for westernblot experiments.

Due to its large size, piccolo requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.

Data sheet 142_113.pdf

References for Piccolo - 142 113

Piccolino is required for ribbon architecture at cochlear inner hair cell synapses and for hearing.
Michanski S, Kapoor R, Steyer AM, Möbius W, Früholz I, Ackermann F, Gültas M, Garner CC, Hamra FK, Neef J, Strenzke N, et al.
EMBO reports (2023) 249: e56702. 142 113 IHC, EM; tested species: rat
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 142 113 IHC; tested species: rat
Piccolino is required for ribbon architecture at cochlear inner hair cell synapses and for hearing.
Michanski S, Kapoor R, Steyer AM, Möbius W, Früholz I, Ackermann F, Gültas M, Garner CC, Hamra FK, Neef J, Strenzke N, et al.
EMBO reports (2023) 249: e56702. 142 113 IHC, EM; tested species: rat
Cat. No.: 142 113
Amount: 50 µg
Price: $375.00
Piccolino is required for ribbon architecture at cochlear inner hair cell synapses and for hearing.
Michanski S, Kapoor R, Steyer AM, Möbius W, Früholz I, Ackermann F, Gültas M, Garner CC, Hamra FK, Neef J, Strenzke N, et al.
EMBO reports (2023) 249: e56702. 142 113 IHC, EM; tested species: rat
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 142 113 IHC; tested species: rat
Piccolino is required for ribbon architecture at cochlear inner hair cell synapses and for hearing.
Michanski S, Kapoor R, Steyer AM, Möbius W, Früholz I, Ackermann F, Gültas M, Garner CC, Hamra FK, Neef J, Strenzke N, et al.
EMBO reports (2023) 249: e56702. 142 113 IHC, EM; tested species: rat
Background

Piccolo, also referred to as Aczonin, is a large protein which constists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains (PBH-domains) and C-terminal PDZ and C2 domains. In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones).
Piccolo is supposed to be a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Recently piccolo has been shown to interfere with clathrin mediated endocytosis by binding to the F-actin and dynamin binding protein Abp1.