Synaptic Systems

Cat. No.: 142 104

Amount: 100 µl

Price: $370.00

Cat. No. 142 104	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 200 gallery IHC-P: 1 : 500 gallery
Immunogen	Recombinant protein corresponding to a central region of rat piccolo (UniProt Id: Q9JKS6)
Reactivity	Reacts with: rat (Q9JKS6), mouse (Q9QYX7). Other species not tested yet.
Specificity	K.O. validated PubMed: 32122952
Remarks	WB: This antibody detects an additional band of ~65 kDa.
Data sheet	142_104.pdf

References for Piccolo - 142 104

WB (1)
ICC (5)
IHC (8)
UPTAKE (1)

Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting.
Awasthi A, Ramachandran B, Ahmed S, Benito E, Shinoda Y, Nitzan N, Heukamp A, Rannio S, Martens H, Barth J, Burk K, et al.
Science (New York, N.Y.) (2018) : . 142 104 WB; tested species: rat

Non-canonical function of ADAM10 in presynaptic plasticity.
Bär J, Fanutza T, Reimann CC, Seipold L, Grohe M, Bolter JR, Delfs F, Bucher M, Gee CE, Schweizer M, Saftig P, et al.
Cellular and molecular life sciences : CMLS (2024) 811: 342. 142 104 ICC; tested species: mouse

Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 142 104 ICC, UPTAKE; tested species: rat

Loss of FEZ1, a gene deleted in Jacobsen syndrome, causes locomotion defects and early mortality by impairing motor neuron development.
Gunaseelan S, Wang Z, Tong VKJ, Ming SWS, Razar RBBA, Srimasorn S, Ong WY, Lim KL, Chua JJE
Human molecular genetics (2021) : . 142 104 ICC; tested species: human

Kinesin Family of Proteins Kif11 and Kif21B Act as Inhibitory Constraints of Excitatory Synaptic Transmission Through Distinct Mechanisms.
Swarnkar S, Avchalumov Y, Raveendra BL, Grinman E, Puthanveettil SV
Scientific reports (2018) 81: 17419. 142 104 ICC; KD verified; tested species: mouse

Effects of ethanol on glycinergic synaptic currents in mouse spinal cord neurons.
Mariqueo TA, Agurto A, Muñoz B, San Martin L, Coronado C, Fernández-Pérez EJ, Murath P, Sánchez A, Homanics GE, Aguayo LG
Journal of neurophysiology (2014) 11110: 1940-8. 142 104 ICC; tested species: mouse

SRF-deficient astrocytes provide neuroprotection in mouse models of excitotoxicity and neurodegeneration.
Thumu SCR, Jain M, Soman S, Das S, Verma V, Nandi A, Gutmann DH, Jayaprakash B, Nair D, Clement JP, Marathe S, et al.
eLife (2024) 13: . 142 104 IHC; tested species: mouse

URMC-099 prophylaxis prevents hippocampal vascular vulnerability and synaptic damage in an orthopedic model of delirium superimposed on dementia.
Miller-Rhodes P, Li H, Velagapudi R, Chiang W, Terrando N, Gelbard HA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2022) 366: e22343. 142 104 IHC; tested species: mouse

Loss of miR-183/96 alters synaptic strength via pre- and postsynaptic mechanisms at a central synapse.
Krohs C, Körber C, Ebbers L, Altaf F, Hollje G, Hoppe S, Dörflinger Y, Prosser HM, Nothwang HG
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) : . 142 104 IHC; tested species: mouse

Excitatory amino acid transporter EAAT5 improves temporal resolution in the retina.
Gehlen J, Aretzweiler C, Mataruga A, Fahlke C, Müller F
eNeuro (2021) : . 142 104 IHC; tested species: mouse

A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 142 104 IHC; tested species: rat

Loss of Piccolo Function in Rats Induces Cerebellar Network Dysfunction and Pontocerebellar Hypoplasia Type 3-like Phenotypes.
Falck J, Bruns C, Hoffmann-Conaway S, Straub I, Plautz EJ, Orlando M, Munawar H, Rivalan M, Winter Y, Izsvák Z, Schmitz D, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 4014: 2943-2959. 142 104 IHC; KO verified; tested species: rat

HIV Tat causes synapse loss in a mouse model of HIV-associated neurocognitive disorder that is independent of the classical complement cascade component C1q.
Hammond JW, Qiu WQ, Marker DF, Chamberlain JM, Greaves-Tunnell W, Bellizzi MJ, Lu SM, Gelbard HA
Glia (2018) : . 142 104 IHC; tested species: mouse

The presynaptic scaffolding protein Piccolo organizes the readily releasable pool at the calyx of Held.
Parthier D, Kuner T, Körber C
The Journal of physiology (2018) 5968: 1485-1499. 142 104 IHC; KD verified; tested species: rat

Cat. No.: 142 104

Amount: 100 µl

Price: $370.00

References
WB (1)
ICC (5)
IHC (8)
UPTAKE (1)

Excitatory amino acid transporter EAAT5 improves temporal resolution in the retina.
Gehlen J, Aretzweiler C, Mataruga A, Fahlke C, Müller F
eNeuro (2021) : . 142 104 IHC; tested species: mouse

ICC

IHC

IHC-P

Background

Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn²⁺ finger, several piccolo-bassoon homology domains (PBH-domains) and C-terminal PDZ and C2 domains. In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matrix assembled at active zones).
Piccolo is supposed to be a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Recently piccolo has been shown to interfere with clathrin mediated endocytosis by binding to the F-actin and dynamin binding protein Abp1.

1 ) Shapira M et al., Neuron (2003)
2 ) Fenster SD et al., J. Biol. Chem. (2003)
3 ) Fenster SD et al., Int. J. Dev. Neurosci. ()
4 ) Dick O et al., J. Comp. Neurol. (2001)
5 ) Gerber SH et al., EMBO J. (2001)
6 ) Fenster SD et al., Neuron (2000)
7 ) Cases-Langhoff C et al., Eur. J. Cell Biol. (1996)

Protocols

AP Detection
ECL Detection
Fluorescent detection
Pre-adsorption protocol

Standard protocol

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

Other products for this target

Piccolo antibody - 142 104 K.O.

References for Piccolo - 142 104