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Parvalbumin antibody - 195 011BT

Parvalbumin is calcium binding protein specific for a subpopulation of GABAergic interneurones
Mouse monoclonal purified IgG
Cat. No.: 195 011BT
Amount: 100 µg
Price: $465.00
Cat. No. 195 011BT 100 µg purified IgG, lyophilized, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: yes (see remarks)
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P: not tested yet
Label biotin
Clone 58E1
Subtype IgG1 (κ light chain)
Immunogen Full-length recombinant rat Parvalbumin (UniProt Id: P02625)
Reactivity Reacts with: rat (P02625), mouse (P32848).
No signal: zebrafish.
Other species not tested yet.
Matching control protein/peptide 195-0P
Remarks

WB: The rabbit polyclonal antiserum (cat. no. 195 002) is recommended for westernblotting. Due to its small size, a tricine gel should be used.

Data sheet 195_011bt.pdf
Cat. No.: 195 011BT
Amount: 100 µg
Price: $465.00
Background

Parvalbumin is a small, acidic calcium binding protein and belongs to the family of EF hand proteins. The protein is found in skeletal muscle and the brain of vertebrates where it locates to a specific population of GABAergic interneurons. This subset of neurons may contribute to maintaining the balance between excitation and inhibition in the cortex and the hippocampus.