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Home > Products > 123 011

NSF antibody - 123 011

NSF is a trimeric ATPase required for membrane fusion during vesicular transport
Mouse monoclonal purified IgG
Cat. No.: 123 011
Amount: 100 µg
Price: $420.00
Cat. No. 123 011 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: not recommended
IHC-P: 1 : 1000 gallery  
ELISA: yes
Clone 83.11
Subtype IgG1
Immunogen Recombinant protein corresponding to AA 1 to 744 from rat NSF (UniProt Id: Q9QUL6)
Reactivity Reacts with: rat (Q9QUL6), mouse (P46460).
No signal: zebrafish.
Other species not tested yet.
Remarks

ELISA: The ELISA-protocol for membrane proteins is required.
Suitable as capture antibody for sandwich-ELISA. Please refer to the protocol for suitable detector antibodies.
Data sheet 123_011.pdf

References for NSF - 123 011

Synapsin Condensates Recruit alpha-Synuclein.
Hoffmann C, Sansevrino R, Morabito G, Logan C, Vabulas RM, Ulusoy A, Ganzella M, Milovanovic D
Journal of molecular biology (2021) 43312: 166961. 123 011 WB; tested species: rat
Autistic-Like Behavior and Impairment of Serotonin Transporter and AMPA Receptor Trafficking in N-Ethylmaleimide Sensitive Factor Gene-Deficient Mice.
Xie MJ, Iwata K, Ishikawa Y, Nomura Y, Tani T, Murata K, Fukazawa Y, Matsuzaki H
Frontiers in genetics (2021) 12: 748627. 123 011 WB, IHC; KD verified; tested species: mouse
LRRK2 G2019S kinase activity triggers neurotoxic NSF aggregation.
Pischedda F, Cirnaru MD, Ponzoni L, Sandre M, Biosa A, Carrion MP, Marin O, Morari M, Pan L, Greggio E, Bandopadhyay R, et al.
Brain : a journal of neurology (2021) 1445: 1509-1525. 123 011 WB, ICC, IHC; tested species: mouse
Synaptic vesicle glycoprotein 2A (SV2A) regulates kindling epileptogenesis via GABAergic neurotransmission.
Tokudome K, Okumura T, Shimizu S, Mashimo T, Takizawa A, Serikawa T, Terada R, Ishihara S, Kunisawa N, Sasa M, Ohno Y, et al.
Scientific reports (2016) 6: 27420. 123 011 WB
CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC
The EMBO journal (2012) 314: 829-41. 123 011 WB; tested species: mouse
Cat. No.: 123 011
Amount: 100 µg
Price: $420.00
WB
ICC
IHC-P
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