NeuN - 266 008
A neuronal specific nuclear protein in vertebrates
Recombinant rabbit purified IgG
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|Cat. No. 266 008||50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: not tested yet
IP: not tested yet
ICC: 1 : 500 gallery
IHC: 1 : 500 gallery
IHC-P/FFPE: 1 : 1000 up to 1 : 5000 (see remarks) gallery
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1 to 97 from mouse NeuN (UniProt Id: Q8BIF2)|
Epitop: AA 5 to 15 from mouse NeuN (UniProt Id: Q8BIF2)
Reacts with: rat (D4A2H6), mouse (Q8BIF2).
No signal: human (A6NFN3).
Other species not tested yet.
|Specificity||Specific for NeuN.|
This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 350D3. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IHC-P: For best results apply short antigen retrieval (5 min, citrate pH 6,0 at 97°C).