Synaptic Systems - N-Cadherin

Cat. No.: 363 003

Amount: 50 µg

Price: $380.00

Cat. No. 363 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 up to 1 : 1000 gallery IHC: external data (see remarks) gallery IHC-P: 1 : 500 up to 1 : 1000 gallery
Immunogen	Recombinant protein corresponding to AA 746 to 906 from mouse N-Cadherin (UniProt Id: P15116)
Reactivity	Reacts with: rat (Q9Z1Y3), mouse (P15116), human (P19022). Other species not tested yet.
Specificity	Recognizes N-cadherin. The antibody may crossreact to other cadherins due to sequence homology.
Remarks	IHC: This antibody has not yet been successfully tested for this application using our standard protocol, but customers used the antibody with mild fixation with 4% PFA and 15% picric acid according to Kirizs et al. 2014 (see gallery).
Data sheet	363_003.pdf

References for N-Cadherin - 363 003

Plk2 promotes synaptic destabilization through disruption of N-cadherin adhesion complexes during homeostatic adaptation to hyperexcitation.
Abdel-Ghani M, Lee Y, Akli LA, Moran M, Schneeweis A, Djemil S, El Choueiry R, Murtadha R, Pak DTS
Journal of neurochemistry (2023) : . 363 003 WB, IP, ICC; tested species: rat

Altered Glutaminase 1 Activity During Neurulation and Its Potential Implications in Neural Tube Defects.
Benavides-Rivas C, Tovar LM, Zúñiga N, Pinto-Borguero I, Retamal C, Yévenes GE, Moraga-Cid G, Fuentealba J, Guzmán L, Coddou C, Bascuñán-Godoy L, et al.
Frontiers in pharmacology (2020) 11: 900. 363 003 WB; tested species: frog

Cat. No.: 363 003

Amount: 50 µg

Price: $380.00

ICC

IHC

IHC-P

Background

N-Cadherin or neural cadherin also known as Cadherin 2 (CDH 2) or NCAD is a type-I classical cadherin, along with E-Cadherin, P-Cadherin, R-Cadherin, and M-Cadherin. This transmembrane protein plays a crucial role in calcium-dependent cell-cell adhesion. In an anti-parallel conformation, its extracellular region interacts with another N-Cadherin molecule on an adjacent cell, forming a linear adhesive "zipper" through repeated dimer interfaces. Its C-terminal, cytoplasmic tail binds to catenins, which in turn link to the actin cytoskeleton (1). N-Cadherin is prevalent in non-epithelial tissues and is expressed in different types of cells such as neural cells, endothelial cells, stromal cells and osteoblasts. It plays an important role in renal epithelial integrity and polarity and is expressed in proximal renal tubules (2). Additionally, N-Cadherin plays a key role in reproductive biology (3) and in myocardium (4). In neural tissue, N-Cadherin replaces E-Cadherin during neurulation, forming strong adherens junctions to maintain tissue architecture and regulates proliferation and differentiation of neural progenitor cells. Cleavage of N-Cadherin modulates adult neural stem cell functional quiescence (5).
N-Cadherin serves as an indicator of ongoing epithelial-to-mesenchymal transition (EMT) and its expression has been correlated with the development of various types of carcinomas. It also promotes angiogenesis and the integrity of blood vessels by ensheathing endothelial and mural cells, stabilizing microvessels (6).

1 ) Vunnam N et al., Biochemistry (2011)
2 ) Terada N et al., J Vet Med Sci (2017)
3 ) Vazquez-Levin MH et al., Dev Biol (2015)
4 ) Kostetskii I et al., Circ Res (2005)
5 ) Porlan E et al., Nat. Cell Biol. (2014)
6 ) Loh CY et al., Cells (2019)

Protocols

N-Cadherin (CD325) antibody - 363 003

References for N-Cadherin - 363 003