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Home > Products > 116 011

Munc18-1 antibody - 116 011

Munc18 proteins are peripheral membrane protein involved in vesicle docking and fusion
Mouse monoclonal purified IgG
Cat. No.: 116 011
Amount: 100 µg
Price: $415.00
Cat. No. 116 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 1000 gallery  
IHC: not recommended
IHC-P: not recommended
ELISA: yes (see remarks)
Clone 131.1
Subtype IgG2a (κ light chain)
Immunogen Full-length rat Munc18-1 recombinant protein (UniProt Id: P61765)
Reactivity Reacts with: rat (P61765), mouse (O08599).
Other species not tested yet.
Specificity Specific for Munc 18-1 with a minor cross-reactivity to Munc 18-2.
Remarks

WB: This antibody detects two smaller bands (possible degradation products) of unkown identity.
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 116 002 as detector antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet 116_011.pdf

References for Munc18-1 - 116 011

Age-dependent neurological phenotypes in a mouse model of PRRT2-related diseases.
Aj F, T M, C I, C BM, Kj N, H L, A N, Sm V, Y-H F, Lj P
Neurogenetics (2021) : . 116 011 WB; tested species: mouse
Maternal stress programs accelerated aging of the basal ganglia motor system in offspring.
Marrocco J, Verhaeghe R, Bucci D, Di Menna L, Traficante A, Bouwalerh H, Van Camp G, Ghiglieri V, Picconi B, Calabresi P, Ravasi L, et al.
Neurobiology of stress (2020) 13: 100265. 116 011 WB; tested species: rat
The reduction in glutamate release is predictive of cognitive and emotional alterations that are corrected by the positive modulator of AMPA receptors S 47445 in perinatal stressed rats.
Morley-Fletcher S, Zuena AR, Mairesse J, Gatta E, Van Camp G, Bouwalerh H, Riozzi B, Battaglia G, Pittaluga A, Olivero G, Mocaer E, et al.
Neuropharmacology (2018) 135: 284-296. 116 011 WB; tested species: rat
Potentiation of excitatory synaptic transmission ameliorates aggression in mice with Stxbp1 haploinsufficiency.
Miyamoto H, Shimohata A, Abe M, Abe T, Mazaki E, Amano K, Suzuki T, Tatsukawa T, Itohara S, Sakimura K, Yamakawa K, et al.
Human molecular genetics (2017) 2624: 4961-4974. 116 011 WB; tested species: mouse
Synaptic vesicle glycoprotein 2A (SV2A) regulates kindling epileptogenesis via GABAergic neurotransmission.
Tokudome K, Okumura T, Shimizu S, Mashimo T, Takizawa A, Serikawa T, Terada R, Ishihara S, Kunisawa N, Sasa M, Ohno Y, et al.
Scientific reports (2016) 6: 27420. 116 011 WB
The effects of antidepressant treatment in prenatally stressed rats support the glutamatergic hypothesis of stress-related disorders.
Marrocco J, Reynaert ML, Gatta E, Gabriel C, Mocaër E, Di Prisco S, Merega E, Pittaluga A, Nicoletti F, Maccari S, Morley-Fletcher S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 346: 2015-24. 116 011 WB; tested species: rat
Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 116 011 WB; tested species: mouse
Anxiety-like behavior of prenatally stressed rats is associated with a selective reduction of glutamate release in the ventral hippocampus.
Marrocco J, Mairesse J, Ngomba RT, Silletti V, Van Camp G, Bouwalerh H, Summa M, Pittaluga A, Nicoletti F, Maccari S, Morley-Fletcher S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3248: 17143-54. 116 011 WB
Munc18-1 regulates first-phase insulin release by promoting granule docking to multiple syntaxin isoforms.
Oh E, Kalwat MA, Kim MJ, Verhage M, Thurmond DC
The Journal of biological chemistry (2012) 28731: 25821-33. 116 011 WB; KO verified; tested species: mouse
Stimulus-induced S-nitrosylation of Syntaxin 4 impacts insulin granule exocytosis.
Wiseman DA, Kalwat MA, Thurmond DC
The Journal of biological chemistry (2011) 28618: 16344-54. 116 011 WB
Single synapse glutamate imaging reveals multiple levels of release mode regulation in mammalian synapses.
Farsi Z, Walde M, Klementowicz AE, Paraskevopoulou F, Woehler A
iScience (2021) 241: 101909. 116 011 ICC; tested species: rat
Riluzole attenuates the efficacy of glutamatergic transmission by interfering with the size of the readily releasable neurotransmitter pool.
Lazarevic V, Yang Y, Ivanova D, Fejtova A, Svenningsson P
Neuropharmacology (2018) : . 116 011 ICC; tested species: rat
Dendritic position is a major determinant of presynaptic strength.
de Jong AP, Schmitz SK, Toonen RF, Verhage M
The Journal of cell biology (2012) 1972: 327-37. 116 011 ICC
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 116 011 ICC
Super-resolution imaging reveals the nanoscale organization of metabotropic glutamate receptors at presynaptic active zones.
Siddig S, Aufmkolk S, Doose S, Jobin ML, Werner C, Sauer M, Calebiro D
Science advances (2020) 616: eaay7193. 116 011 IHC; tested species: mouse
Cat. No.: 116 011
Amount: 100 µg
Price: $415.00
Age-dependent neurological phenotypes in a mouse model of PRRT2-related diseases.
Aj F, T M, C I, C BM, Kj N, H L, A N, Sm V, Y-H F, Lj P
Neurogenetics (2021) : . 116 011 WB; tested species: mouse
Maternal stress programs accelerated aging of the basal ganglia motor system in offspring.
Marrocco J, Verhaeghe R, Bucci D, Di Menna L, Traficante A, Bouwalerh H, Van Camp G, Ghiglieri V, Picconi B, Calabresi P, Ravasi L, et al.
Neurobiology of stress (2020) 13: 100265. 116 011 WB; tested species: rat
The reduction in glutamate release is predictive of cognitive and emotional alterations that are corrected by the positive modulator of AMPA receptors S 47445 in perinatal stressed rats.
Morley-Fletcher S, Zuena AR, Mairesse J, Gatta E, Van Camp G, Bouwalerh H, Riozzi B, Battaglia G, Pittaluga A, Olivero G, Mocaer E, et al.
Neuropharmacology (2018) 135: 284-296. 116 011 WB; tested species: rat
Potentiation of excitatory synaptic transmission ameliorates aggression in mice with Stxbp1 haploinsufficiency.
Miyamoto H, Shimohata A, Abe M, Abe T, Mazaki E, Amano K, Suzuki T, Tatsukawa T, Itohara S, Sakimura K, Yamakawa K, et al.
Human molecular genetics (2017) 2624: 4961-4974. 116 011 WB; tested species: mouse
Synaptic vesicle glycoprotein 2A (SV2A) regulates kindling epileptogenesis via GABAergic neurotransmission.
Tokudome K, Okumura T, Shimizu S, Mashimo T, Takizawa A, Serikawa T, Terada R, Ishihara S, Kunisawa N, Sasa M, Ohno Y, et al.
Scientific reports (2016) 6: 27420. 116 011 WB
The effects of antidepressant treatment in prenatally stressed rats support the glutamatergic hypothesis of stress-related disorders.
Marrocco J, Reynaert ML, Gatta E, Gabriel C, Mocaër E, Di Prisco S, Merega E, Pittaluga A, Nicoletti F, Maccari S, Morley-Fletcher S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 346: 2015-24. 116 011 WB; tested species: rat
Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 116 011 WB; tested species: mouse
Anxiety-like behavior of prenatally stressed rats is associated with a selective reduction of glutamate release in the ventral hippocampus.
Marrocco J, Mairesse J, Ngomba RT, Silletti V, Van Camp G, Bouwalerh H, Summa M, Pittaluga A, Nicoletti F, Maccari S, Morley-Fletcher S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3248: 17143-54. 116 011 WB
Munc18-1 regulates first-phase insulin release by promoting granule docking to multiple syntaxin isoforms.
Oh E, Kalwat MA, Kim MJ, Verhage M, Thurmond DC
The Journal of biological chemistry (2012) 28731: 25821-33. 116 011 WB; KO verified; tested species: mouse
Stimulus-induced S-nitrosylation of Syntaxin 4 impacts insulin granule exocytosis.
Wiseman DA, Kalwat MA, Thurmond DC
The Journal of biological chemistry (2011) 28618: 16344-54. 116 011 WB
Single synapse glutamate imaging reveals multiple levels of release mode regulation in mammalian synapses.
Farsi Z, Walde M, Klementowicz AE, Paraskevopoulou F, Woehler A
iScience (2021) 241: 101909. 116 011 ICC; tested species: rat
Riluzole attenuates the efficacy of glutamatergic transmission by interfering with the size of the readily releasable neurotransmitter pool.
Lazarevic V, Yang Y, Ivanova D, Fejtova A, Svenningsson P
Neuropharmacology (2018) : . 116 011 ICC; tested species: rat
Dendritic position is a major determinant of presynaptic strength.
de Jong AP, Schmitz SK, Toonen RF, Verhage M
The Journal of cell biology (2012) 1972: 327-37. 116 011 ICC
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 116 011 ICC
Super-resolution imaging reveals the nanoscale organization of metabotropic glutamate receptors at presynaptic active zones.
Siddig S, Aufmkolk S, Doose S, Jobin ML, Werner C, Sauer M, Calebiro D
Science advances (2020) 616: eaay7193. 116 011 IHC; tested species: mouse
WB
ICC
Background

Munc 18 is an abundant neuronal protein that tightly binds to the synaptic fusion protein syntaxin 1. It is highly homologous to the C. elegans unc-18 gene product, and weakly related to the yeast sec1, sly1, and slp1 genes.
There are three munc 18 isoforms in mammals. Munc 18-1 or 18a, also referred to as rb-sec1, n-sec1, stxbp1 and p67, is primarily expressed in neurons. Munc 18-2 or 18b, also referred to as stxbp2, and Munc 18-3 or 18c are expressed ubiquitously.
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