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Home > Products > 126 111

Munc13-1 antibody - 126 111 K.O.

Munc13 proteins are the mammalian homologues of the C. elegans unc-13 gene essential for exocytosis
Mouse monoclonal purified IgG
Cat. No.: 126 111
Amount: 100 µg
Price: $415.00
Cat. No. 126 111 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 up to 1 : 5000 (AP staining) gallery  
IP: yes (see remarks)
ICC: not tested yet
IHC: not tested yet
IHC-P: not tested yet
ELISA: yes (see remarks)
Clone 266B1
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 3 to 317 from rat Munc13-1 (UniProt Id: Q62768)
Reactivity Reacts with: rat (Q62768), mouse (Q4KUS2), zebrafish.
Other species not tested yet.
Specificity K.O. validated
Remarks

IP: For most effective IP, use the denaturing IP-protocol. Consider that protein-protein interactions may be affected.
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 126 103 as detector antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet 126_111.pdf

References for Munc13-1 - 126 111

A presynaptic phosphosignaling hub for lasting homeostatic plasticity.
Müller JA, Betzin J, Santos-Tejedor J, Mayer A, Oprişoreanu AM, Engholm-Keller K, Paulußen I, Gulakova P, McGovern TD, Gschossman LJ, Schönhense E, et al.
Cell reports (2022) 393: 110696. 126 111 WB; tested species: mouse
CYP46A1 Activation by Efavirenz Leads to Behavioral Improvement without Significant Changes in Amyloid Plaque Load in the Brain of 5XFAD Mice.
Petrov AM, Lam M, Mast N, Moon J, Li Y, Maxfield E, Pikuleva IA
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics (2019) : . 126 111 WB; tested species: mouse
RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses.
Grauel MK, Maglione M, Reddy-Alla S, Willmes CG, Brockmann MM, Trimbuch T, Rosenmund T, Pangalos M, Vardar G, Stumpf A, Walter AM, et al.
Proceedings of the National Academy of Sciences of the United States of America (2016) 11341: 11615-11620. 126 111 WB; tested species: mouse
Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP.
Kaeser-Woo YJ, Younts TJ, Yang X, Zhou P, Wu D, Castillo PE, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3323: 9769-80. 126 111 WB
Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 126 111 WB; tested species: mouse
Formation of Golgi-derived active zone precursor vesicles.
Maas C, Torres VI, Altrock WD, Leal-Ortiz S, Wagh D, Terry-Lorenzo RT, Fejtova A, Gundelfinger ED, Ziv NE, Garner CC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3232: 11095-108. 126 111 WB, ICC
Munc18-1 regulates first-phase insulin release by promoting granule docking to multiple syntaxin isoforms.
Oh E, Kalwat MA, Kim MJ, Verhage M, Thurmond DC
The Journal of biological chemistry (2012) 28731: 25821-33. 126 111 WB; tested species: mouse
Doc2b is a high-affinity Ca2+ sensor for spontaneous neurotransmitter release.
Groffen AJ, Martens S, Díez Arazola R, Cornelisse LN, Lozovaya N, de Jong AP, Goriounova NA, Habets RL, Takai Y, Borst JG, Brose N, et al.
Science (New York, N.Y.) (2010) 3275973: 1614-8. 126 111 WB; tested species: mouse
Munc13-4 is a limiting factor in the pathway required for platelet granule release and hemostasis.
Ren Q, Wimmer C, Chicka MC, Ye S, Ren Y, Hughson FM, Whiteheart SW
Blood (2010) 1166: 869-77. 126 111 WB; tested species: mouse
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 126 111 WB
Active zone protein CAST is a component of conventional and ribbon synapses in mouse retina.
Deguchi-Tawarada M, Inoue E, Takao-Rikitsu E, Inoue M, Kitajima I, Ohtsuka T, Takai Y
The Journal of comparative neurology (2006) 4954: 480-96. 126 111 WB; tested species: mouse
Cast: a novel protein of the cytomatrix at the active zone of synapses that forms a ternary complex with RIM1 and munc13-1.
Ohtsuka T, Takao-Rikitsu E, Inoue E, Inoue M, Takeuchi M, Matsubara K, Deguchi-Tawarada M, Satoh K, Morimoto K, Nakanishi H, Takai Y, et al.
The Journal of cell biology (2002) 1583: 577-90. 126 111 WB; tested species: rat
Different Munc13 isoforms function as priming factors in lytic granule release from murine cytotoxic T lymphocytes.
Dudenhöffer-Pfeifer M, Schirra C, Pattu V, Halimani M, Maier-Peuschel M, Marshall MR, Matti U, Becherer U, Dirks J, Jung M, Lipp P, et al.
Traffic (Copenhagen, Denmark) (2013) 147: 798-809. 126 111 IP; tested species: mouse
The metabotropic glutamate receptor mGlu7 activates phospholipase C, translocates munc-13-1 protein, and potentiates glutamate release at cerebrocortical nerve terminals.
Martín R, Durroux T, Ciruela F, Torres M, Pin JP, Sánchez-Prieto J
The Journal of biological chemistry (2010) 28523: 17907-17. 126 111 IP
Vti1a/b regulate synaptic vesicle and dense core vesicle secretion via protein sorting at the Golgi.
Emperador-Melero J, Huson V, van Weering J, Bollmann C, Fischer von Mollard G, Toonen RF, Verhage M
Nature communications (2018) 91: 3421. 126 111 ICC; tested species: mouse
RIM C2B Domains Target Presynaptic Active Zone Functions to PIP2-Containing Membranes.
de Jong APH, Roggero CM, Ho MR, Wong MY, Brautigam CA, Rizo J, Kaeser PS
Neuron (2018) 982: 335-349.e7. 126 111 ICC; tested species: mouse
Studying synaptic efficiency by post-hoc immunolabelling.
Ramírez-Franco J, Alonso B, Bartolomé-Martín D, Sánchez-Prieto J, Torres M
BMC neuroscience (2013) 14: 127. 126 111 ICC
Formation of Golgi-derived active zone precursor vesicles.
Maas C, Torres VI, Altrock WD, Leal-Ortiz S, Wagh D, Terry-Lorenzo RT, Fejtova A, Gundelfinger ED, Ziv NE, Garner CC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3232: 11095-108. 126 111 WB, ICC
Efficient synaptic vesicle recycling after intense exocytosis concomitant with the accumulation of non-releasable endosomes at early developmental stages.
Bartolomé-Martín D, Ramírez-Franco J, Castro E, Sánchez-Prieto J, Torres M
Journal of cell science (2012) 125Pt 2: 422-34. 126 111 ICC; tested species: rat
Non-additive potentiation of glutamate release by phorbol esters and metabotropic mGlu7 receptor in cerebrocortical nerve terminals.
Martín R, Bartolomé-Martín D, Torres M, Sánchez-Prieto J
Journal of neurochemistry (2011) 1164: 476-85. 126 111 ICC; tested species: mouse
Cat. No.: 126 111
Amount: 100 µg
Price: $415.00
A presynaptic phosphosignaling hub for lasting homeostatic plasticity.
Müller JA, Betzin J, Santos-Tejedor J, Mayer A, Oprişoreanu AM, Engholm-Keller K, Paulußen I, Gulakova P, McGovern TD, Gschossman LJ, Schönhense E, et al.
Cell reports (2022) 393: 110696. 126 111 WB; tested species: mouse
CYP46A1 Activation by Efavirenz Leads to Behavioral Improvement without Significant Changes in Amyloid Plaque Load in the Brain of 5XFAD Mice.
Petrov AM, Lam M, Mast N, Moon J, Li Y, Maxfield E, Pikuleva IA
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics (2019) : . 126 111 WB; tested species: mouse
RIM-binding protein 2 regulates release probability by fine-tuning calcium channel localization at murine hippocampal synapses.
Grauel MK, Maglione M, Reddy-Alla S, Willmes CG, Brockmann MM, Trimbuch T, Rosenmund T, Pangalos M, Vardar G, Stumpf A, Walter AM, et al.
Proceedings of the National Academy of Sciences of the United States of America (2016) 11341: 11615-11620. 126 111 WB; tested species: mouse
Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP.
Kaeser-Woo YJ, Younts TJ, Yang X, Zhou P, Wu D, Castillo PE, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3323: 9769-80. 126 111 WB
Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 126 111 WB; tested species: mouse
Formation of Golgi-derived active zone precursor vesicles.
Maas C, Torres VI, Altrock WD, Leal-Ortiz S, Wagh D, Terry-Lorenzo RT, Fejtova A, Gundelfinger ED, Ziv NE, Garner CC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3232: 11095-108. 126 111 WB, ICC
Munc18-1 regulates first-phase insulin release by promoting granule docking to multiple syntaxin isoforms.
Oh E, Kalwat MA, Kim MJ, Verhage M, Thurmond DC
The Journal of biological chemistry (2012) 28731: 25821-33. 126 111 WB; tested species: mouse
Doc2b is a high-affinity Ca2+ sensor for spontaneous neurotransmitter release.
Groffen AJ, Martens S, Díez Arazola R, Cornelisse LN, Lozovaya N, de Jong AP, Goriounova NA, Habets RL, Takai Y, Borst JG, Brose N, et al.
Science (New York, N.Y.) (2010) 3275973: 1614-8. 126 111 WB; tested species: mouse
Munc13-4 is a limiting factor in the pathway required for platelet granule release and hemostasis.
Ren Q, Wimmer C, Chicka MC, Ye S, Ren Y, Hughson FM, Whiteheart SW
Blood (2010) 1166: 869-77. 126 111 WB; tested species: mouse
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 126 111 WB
Active zone protein CAST is a component of conventional and ribbon synapses in mouse retina.
Deguchi-Tawarada M, Inoue E, Takao-Rikitsu E, Inoue M, Kitajima I, Ohtsuka T, Takai Y
The Journal of comparative neurology (2006) 4954: 480-96. 126 111 WB; tested species: mouse
Cast: a novel protein of the cytomatrix at the active zone of synapses that forms a ternary complex with RIM1 and munc13-1.
Ohtsuka T, Takao-Rikitsu E, Inoue E, Inoue M, Takeuchi M, Matsubara K, Deguchi-Tawarada M, Satoh K, Morimoto K, Nakanishi H, Takai Y, et al.
The Journal of cell biology (2002) 1583: 577-90. 126 111 WB; tested species: rat
Different Munc13 isoforms function as priming factors in lytic granule release from murine cytotoxic T lymphocytes.
Dudenhöffer-Pfeifer M, Schirra C, Pattu V, Halimani M, Maier-Peuschel M, Marshall MR, Matti U, Becherer U, Dirks J, Jung M, Lipp P, et al.
Traffic (Copenhagen, Denmark) (2013) 147: 798-809. 126 111 IP; tested species: mouse
The metabotropic glutamate receptor mGlu7 activates phospholipase C, translocates munc-13-1 protein, and potentiates glutamate release at cerebrocortical nerve terminals.
Martín R, Durroux T, Ciruela F, Torres M, Pin JP, Sánchez-Prieto J
The Journal of biological chemistry (2010) 28523: 17907-17. 126 111 IP
Vti1a/b regulate synaptic vesicle and dense core vesicle secretion via protein sorting at the Golgi.
Emperador-Melero J, Huson V, van Weering J, Bollmann C, Fischer von Mollard G, Toonen RF, Verhage M
Nature communications (2018) 91: 3421. 126 111 ICC; tested species: mouse
RIM C2B Domains Target Presynaptic Active Zone Functions to PIP2-Containing Membranes.
de Jong APH, Roggero CM, Ho MR, Wong MY, Brautigam CA, Rizo J, Kaeser PS
Neuron (2018) 982: 335-349.e7. 126 111 ICC; tested species: mouse
Studying synaptic efficiency by post-hoc immunolabelling.
Ramírez-Franco J, Alonso B, Bartolomé-Martín D, Sánchez-Prieto J, Torres M
BMC neuroscience (2013) 14: 127. 126 111 ICC
Formation of Golgi-derived active zone precursor vesicles.
Maas C, Torres VI, Altrock WD, Leal-Ortiz S, Wagh D, Terry-Lorenzo RT, Fejtova A, Gundelfinger ED, Ziv NE, Garner CC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3232: 11095-108. 126 111 WB, ICC
Efficient synaptic vesicle recycling after intense exocytosis concomitant with the accumulation of non-releasable endosomes at early developmental stages.
Bartolomé-Martín D, Ramírez-Franco J, Castro E, Sánchez-Prieto J, Torres M
Journal of cell science (2012) 125Pt 2: 422-34. 126 111 ICC; tested species: rat
Non-additive potentiation of glutamate release by phorbol esters and metabotropic mGlu7 receptor in cerebrocortical nerve terminals.
Martín R, Bartolomé-Martín D, Torres M, Sánchez-Prieto J
Journal of neurochemistry (2011) 1164: 476-85. 126 111 ICC; tested species: mouse
WB
Background
Munc 13s are homologues of the C. elegans unc-13 gene product. Three brain specific isoforms, Munc 13-1, -13-2, and -13-3 are expressed in rat where they localize to presynaptic terminals. All three isoforms share multiple regulatory domains that may mediate phorbol ester and diacylglycerol binding.
Munc13-1 shows the broadest expression pattern and is found in cortex, cerebellum, olfactory bulb and hippocampus. Munc 13-2 is mainly expressed in cortex and hippocampus whereas Munc 13-3 exhibits highest expression levels in cerebellum and pons. Munc13-1 interacts directly with a putative coiled coil domain in the N-terminal part of syntaxin and is involved in synaptic vesicle priming. For Munc13-2 an additional ubiquitously expressed N-terminal splice variant (ubMunc 13-2) has been described.
Munc 13-3 has been shown to be involved in the regulation of cerebellar synaptic transmission and motor learning.
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