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Munc 13-1 - 126 111

Mammalian homologues of the C. elegans unc-13 gene essential for exocytosis

Monoclonal mouse purified IgG
415.00 USD
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Cat. No. 126 111 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 500 up to 1 : 5000 (AP staining) gallery  
IP: yes (see remarks)
ICC: not tested yet (see remarks)
IHC: not tested yet
IHC-P/FFPE: not tested yet
ELISA: yes (see remarks)
Clone 266B1
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 3 to 317 from rat Munc13-1 (UniProt Id: Q62768)
Epitop Epitop: AA 3 to 317 from rat Munc13-1 (UniProt Id: Q62768)
Reactivity Reacts with: rat (Q62768), mouse (Q4KUS2), zebrafish.
Other species not tested yet.
Specificity Specific for munc 13-1. (K.O. verified)
Remarks

IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected.

ICC: limited to methanol fixation.

ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 126 103 as detector antibody (protocol for sandwich-ELISA).

Datasheet 126_111.pdf


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References for Munc 13-1 - 126 111

CYP46A1 Activation by Efavirenz Leads to Behavioral Improvement without Significant Changes in Amyloid Plaque Load in the Brain of 5XFAD Mice.
Petrov AM, Lam M, Mast N, Moon J, Li Y, Maxfield E, Pikuleva IA
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics (2019) : . 126 111 WB; tested species: mouse
Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP.
Kaeser-Woo YJ, Younts TJ, Yang X, Zhou P, Wu D, Castillo PE, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3323: 9769-80. 126 111 WB
Formation of Golgi-derived active zone precursor vesicles.
Maas C, Torres VI, Altrock WD, Leal-Ortiz S, Wagh D, Terry-Lorenzo RT, Fejtova A, Gundelfinger ED, Ziv NE, Garner CC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3232: 11095-108. 126 111 WB, ICC
Munc18-1 regulates first-phase insulin release by promoting granule docking to multiple syntaxin isoforms.
Oh E, Kalwat MA, Kim MJ, Verhage M, Thurmond DC
The Journal of biological chemistry (2012) 28731: 25821-33. 126 111 WB; tested species: mouse
Doc2b is a high-affinity Ca2+ sensor for spontaneous neurotransmitter release.
Groffen AJ, Martens S, Díez Arazola R, Cornelisse LN, Lozovaya N, de Jong AP, Goriounova NA, Habets RL, Takai Y, Borst JG, Brose N, et al.
Science (New York, N.Y.) (2010) 3275973: 1614-8. 126 111 WB; tested species: mouse
Munc13-4 is a limiting factor in the pathway required for platelet granule release and hemostasis.
Ren Q, Wimmer C, Chicka MC, Ye S, Ren Y, Hughson FM, Whiteheart SW
Blood (2010) 1166: 869-77. 126 111 WB; tested species: mouse
Molecular anatomy of a trafficking organelle.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, et al.
Cell (2006) 1274: 831-46. 126 111 WB
Active zone protein CAST is a component of conventional and ribbon synapses in mouse retina.
Deguchi-Tawarada M, Inoue E, Takao-Rikitsu E, Inoue M, Kitajima I, Ohtsuka T, Takai Y
The Journal of comparative neurology (2006) 4954: 480-96. 126 111 WB; tested species: mouse
Different Munc13 isoforms function as priming factors in lytic granule release from murine cytotoxic T lymphocytes.
Dudenhöffer-Pfeifer M, Schirra C, Pattu V, Halimani M, Maier-Peuschel M, Marshall MR, Matti U, Becherer U, Dirks J, Jung M, Lipp P, et al.
Traffic (Copenhagen, Denmark) (2013) 147: 798-809. 126 111 IP; tested species: mouse
The metabotropic glutamate receptor mGlu7 activates phospholipase C, translocates munc-13-1 protein, and potentiates glutamate release at cerebrocortical nerve terminals.
Martín R, Durroux T, Ciruela F, Torres M, Pin JP, Sánchez-Prieto J
The Journal of biological chemistry (2010) 28523: 17907-17. 126 111 IP
Vti1a/b regulate synaptic vesicle and dense core vesicle secretion via protein sorting at the Golgi.
Emperador-Melero J, Huson V, van Weering J, Bollmann C, Fischer von Mollard G, Toonen RF, Verhage M
Nature communications (2018) 91: 3421. 126 111 ICC; tested species: mouse
RIM C2B Domains Target Presynaptic Active Zone Functions to PIP2-Containing Membranes.
de Jong APH, Roggero CM, Ho MR, Wong MY, Brautigam CA, Rizo J, Kaeser PS
Neuron (2018) 982: 335-349.e7. 126 111 ICC; tested species: mouse
Studying synaptic efficiency by post-hoc immunolabelling.
Ramírez-Franco J, Alonso B, Bartolomé-Martín D, Sánchez-Prieto J, Torres M
BMC neuroscience (2013) 14: 127. 126 111 ICC
Formation of Golgi-derived active zone precursor vesicles.
Maas C, Torres VI, Altrock WD, Leal-Ortiz S, Wagh D, Terry-Lorenzo RT, Fejtova A, Gundelfinger ED, Ziv NE, Garner CC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3232: 11095-108. 126 111 WB, ICC
Efficient synaptic vesicle recycling after intense exocytosis concomitant with the accumulation of non-releasable endosomes at early developmental stages.
Bartolomé-Martín D, Ramírez-Franco J, Castro E, Sánchez-Prieto J, Torres M
Journal of cell science (2012) 125Pt 2: 422-34. 126 111 ICC; tested species: rat
Non-additive potentiation of glutamate release by phorbol esters and metabotropic mGlu7 receptor in cerebrocortical nerve terminals.
Martín R, Bartolomé-Martín D, Torres M, Sánchez-Prieto J
Journal of neurochemistry (2011) 1164: 476-85. 126 111 ICC; tested species: mouse
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com