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Munc 13-1 - 126 103

Mammalian homologues of the C. elegans unc-13 gene essential for exocytosis

Polyclonal rabbit purified antibody
450.00 USD
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Cat. No. 126 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: yes (see remarks)
ICC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC: 1 : 200 gallery  
IHC-P/FFPE: not tested yet
EM: yes gallery  
ELISA: yes (see remarks)
Immunogen Recombinant protein corresponding to AA 3 to 317 from rat Munc13-1 (UniProt Id: Q62768)
Reactivity Reacts with: human (Q9UPW8), rat (Q62768), mouse (Q4KUS2), zebrafish.
Other species not tested yet.
Specificity Specific for munc 13-1. (K.O. verified)
Remarks

IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected.

ICC: This antibody gives much better results in ICC than the monoclonal antibody.

ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 126 111 as capture antibody (protocol for sandwich-ELISA).

Datasheet 126_103.pdf


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References for Munc 13-1 - 126 103

Autaptic cultures of human induced neurons as a versatile platform for studying synaptic function and neuronal morphology.
Fenske P, Grauel MK, Brockmann MM, Dorrn AL, Trimbuch T, Rosenmund C
Scientific reports (2019) 91: 4890. 126 103 WB; KD verified; tested species: human
Postsynaptic RIM1 modulates synaptic function by facilitating membrane delivery of recycling NMDARs in hippocampal neurons.
Wang J, Lv X, Wu Y, Xu T, Jiao M, Yang R, Li X, Chen M, Yan Y, Chen C, Dong W, et al.
Nature communications (2018) 91: 2267. 126 103 WB; tested species: mouse
Inhibitory role of Munc13-1 in antigen-induced mast cell degranulation.
Higashio H, Satoh YI, Saino T
Biomedical research (Tokyo, Japan) (2017) 386: 321-329. 126 103 WB; tested species: rat
Molecular Mechanisms of Synaptic Vesicle Priming by Munc13 and Munc18.
Lai Y, Choi UB, Leitz J, Rhee HJ, Lee C, Altas B, Zhao M, Pfuetzner RA, Wang AL, Brose N, Rhee J, et al.
Neuron (2017) 953: 591-607.e10. 126 103 WB; KO verified; tested species: mouse
How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 126 103 WB
The Munc13 proteins differentially regulate readily releasable pool dynamics and calcium-dependent recovery at a central synapse.
Chen Z, Cooper B, Kalla S, Varoqueaux F, Young SM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3319: 8336-51. 126 103 WB
Different Munc13 isoforms function as priming factors in lytic granule release from murine cytotoxic T lymphocytes.
Dudenhöffer-Pfeifer M, Schirra C, Pattu V, Halimani M, Maier-Peuschel M, Marshall MR, Matti U, Becherer U, Dirks J, Jung M, Lipp P, et al.
Traffic (Copenhagen, Denmark) (2013) 147: 798-809. 126 103 WB
β-Adrenergic receptors activate exchange protein directly activated by cAMP (Epac), translocate Munc13-1, and enhance the Rab3A-RIM1α interaction to potentiate glutamate release at cerebrocortical nerve terminals.
Ferrero JJ, Alvarez AM, Ramírez-Franco J, Godino MC, Bartolomé-Martín D, Aguado C, Torres M, Luján R, Ciruela F, Sánchez-Prieto J
The Journal of biological chemistry (2013) 28843: 31370-85. 126 103 WB; tested species: mouse
RIM, Munc13, and Rab3A interplay in acrosomal exocytosis.
Bello OD, Zanetti MN, Mayorga LS, Michaut MA
Experimental cell research (2012) 3185: 478-88. 126 103 WB, ICC
Non-additive potentiation of glutamate release by phorbol esters and metabotropic mGlu7 receptor in cerebrocortical nerve terminals.
Martín R, Bartolomé-Martín D, Torres M, Sánchez-Prieto J
Journal of neurochemistry (2011) 1164: 476-85. 126 103 WB, ICC; tested species: mouse
Synaptic neurexin-1 assembles into dynamically regulated active zone nanoclusters.
Trotter JH, Hao J, Maxeiner S, Tsetsenis T, Liu Z, Zhuang X, Südhof TC
The Journal of cell biology (2019) : . 126 103 ICC; tested species: mouse
Transient Confinement of CaV2.1 Ca2+-Channel Splice Variants Shapes Synaptic Short-Term Plasticity.
Heck J, Parutto P, Ciuraszkiewicz A, Bikbaev A, Freund R, Mitlöhner J, Alonso M, Fejtova A, Holcman D, Heine M
Neuron (2019) : . 126 103 ICC; tested species: rat
Dopamine Secretion Is Mediated by Sparse Active Zone-like Release Sites.
Liu C, Kershberg L, Wang J, Schneeberger S, Kaeser PS
Cell (2018) 1724: 706-718.e15. 126 103 ICC; tested species: mouse
Riluzole attenuates the efficacy of glutamatergic transmission by interfering with the size of the readily releasable neurotransmitter pool.
Lazarevic V, Yang Y, Ivanova D, Fejtova A, Svenningsson P
Neuropharmacology (2018) : . 126 103 ICC; tested species: rat
Visualization of Synchronous or Asynchronous Release of Single Synaptic Vesicle in Active-Zone-Like Membrane Formed on Neuroligin-Coated Glass Surface.
Funahashi J, Tanaka H, Hirano T
Frontiers in cellular neuroscience (2018) 12: 140. 126 103 ICC; tested species: rat
CAPS-1 promotes fusion competence of stationary dense-core vesicles in presynaptic terminals of mammalian neurons.
Farina M, van de Bospoort R, He E, Persoon CM, van Weering JR, Broeke JH, Verhage M, Toonen RF
eLife (2015) 4: . 126 103 ICC
RIM, Munc13, and Rab3A interplay in acrosomal exocytosis.
Bello OD, Zanetti MN, Mayorga LS, Michaut MA
Experimental cell research (2012) 3185: 478-88. 126 103 WB, ICC
Non-additive potentiation of glutamate release by phorbol esters and metabotropic mGlu7 receptor in cerebrocortical nerve terminals.
Martín R, Bartolomé-Martín D, Torres M, Sánchez-Prieto J
Journal of neurochemistry (2011) 1164: 476-85. 126 103 WB, ICC; tested species: mouse
A role for the ubiquitin-proteasome system in activity-dependent presynaptic silencing.
Jiang X, Litkowski PE, Taylor AA, Lin Y, Snider BJ, Moulder KL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 305: 1798-809. 126 103 ICC
Dynein light chain regulates axonal trafficking and synaptic levels of Bassoon.
Fejtova A, Davydova D, Bischof F, Lazarevic V, Altrock WD, Romorini S, Schöne C, Zuschratter W, Kreutz MR, Garner CC, Ziv NE, et al.
The Journal of cell biology (2009) 1852: 341-55. 126 103 ICC
Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis.
Leal-Ortiz S, Waites CL, Terry-Lorenzo R, Zamorano P, Gundelfinger ED, Garner CC
The Journal of cell biology (2008) 1815: 831-46. 126 103 ICC
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 126 103 IHC; tested species: mouse
Bassoon-disruption slows vesicle replenishment and induces homeostatic plasticity at a CNS synapse.
Mendoza Schulz A, Jing Z, Sánchez Caro JM, Wetzel F, Dresbach T, Strenzke N, Wichmann C, Moser T
The EMBO journal (2014) 335: 512-27. 126 103 IHC
Deficits in morphofunctional maturation of hippocampal mossy fiber synapses in a mouse model of intellectual disability.
Lanore F, Labrousse VF, Szabo Z, Normand E, Blanchet C, Mulle C
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3249: 17882-93. 126 103 IHC
Impaired development of hippocampal mossy fibre synapses in mouse mutants for the presynaptic scaffold protein Bassoon.
Lanore F, Blanchet C, Fejtova A, Pinheiro P, Richter K, Balschun D, Gundelfinger E, Mulle C
The Journal of physiology (2010) 588Pt 12: 2133-45. 126 103 IHC; tested species: mouse
Active zone proteins are dynamically associated with synaptic ribbons in rat pinealocytes.
Spiwoks-Becker I, Maus C, tom Dieck S, Fejtová A, Engel L, Wolloscheck T, Wolfrum U, Vollrath L, Spessert R
Cell and tissue research (2008) 3332: 185-95. 126 103 IHC
Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T
The European journal of neuroscience (2012) 368: 3005-20. 126 103 ELISA
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com