Munc 13-1 - 126 102
Mammalian homologues of the C. elegans unc-13 gene essential for exocytosis
Polyclonal rabbit antiserum
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|Cat. No. 126 102||200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) gallery
IP: yes (see remarks)
ICC: 1 : 500 (see remarks) gallery
IHC: 1 : 200 gallery
IHC-P/FFPE: not tested yet
ELISA: (see remarks)
|Immunogen||Recombinant protein corresponding to AA 3 to 317 from rat Munc13-1 (UniProt Id: Q62768)|
Reacts with: human (Q9UPW8), rat (Q62768), mouse (Q4KUS2), zebrafish.
Other species not tested yet.
|Specificity||Specific for munc 13-1. (K.O. verified)|
IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected.
ICC: This antibody gives much better results in ICC than the monoclonal antibody.
ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 126 111 as capture antibody (protocol for sandwich-ELISA).