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SY SY - Synaptic Systems SY SY - Synaptic Systems

MLC-2A - 311 011

A myosin specific for the atrium of the mammalian heart

Monoclonal mouse purified IgG
415.00 USD
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Cat. No. 311 011 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 100 up to 1 : 2000 (AP staining) blot  
IP: not tested yet
ICC: yes (see remarks) image  
IHC: yes (see remarks)
IHC-P/FFPE: 1 : 200 up to 1 : 1000 image 1  image 2  
Clone 56F5
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 175 from human MLC-2A (UniProt Id: Q01449)
Epitop Epitop: AA 1 to 175 from human MLC-2A (UniProt Id: Q01449)
Reactivity Reacts with: human (Q01449), rat, mouse (Q9QVP4).
No signal: chicken.
Other species not tested yet.
Specificity Specific for MLC-2A, no cross-reactivity to MLC-2V.
matching control protein/peptide 311-0P
Remarks

ICC: Only methanol fixation, recommended protocol.

IHC: Protocol for immunohistochemistry of this antibody will be provided with the goods.

Datasheet 311_011.pdf


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References for MLC-2A - 311 011

Phosphorylation and translocation of heat shock protein 27 and alphaB-crystallin in human myocardium after cardioplegia and cardiopulmonary bypass.
Clements RT, Sodha NR, Feng J, Mieno S, Boodhwani M, Ramlawi B, Bianchi C, Sellke FW
The Journal of thoracic and cardiovascular surgery (2007) 1346: 1461-70. 311 011 WB, IHC; tested species: human
Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes.
Lin Y, Linask KL, Mallon B, Johnson K, Klein M, Beers J, Xie W, Du Y, Liu C, Lai Y, Zou J, et al.
Stem cells translational medicine (2017) 62: 527-538. 311 011 ICC; tested species: human
Use of Human Pluripotent Stem Cell Derived-Cardiomyocytes to Study Drug-Induced Cardiotoxicity.
Maillet A, Tan KP, Brunham LR
Current protocols in toxicology (2017) 73: 22.5.1-22.5.22. 311 011 ICC; tested species: human
Generation of multipotent induced cardiac progenitor cells from mouse fibroblasts and potency testing in ex vivo mouse embryos.
Lalit PA, Rodriguez AM, Downs KM, Kamp TJ
Nature protocols (2017) 125: 1029-1054. 311 011 ICC
Chemical-defined and albumin-free generation of human atrial and ventricular myocytes from human pluripotent stem cells.
Pei F, Jiang J, Bai S, Cao H, Tian L, Zhao Y, Yang C, Dong H, Ma Y
Stem cell research (2017) 19: 94-103. 311 011 ICC
Generation of clinical-grade functional cardiomyocytes from human embryonic stem cells in chemically defined conditions.
Tan Y, Han P, Gu Q, Chen G, Wang L, Ma R, Wu J, Feng C, Zhang Y, Wang L, Hu B, et al.
Journal of tissue engineering and regenerative medicine (2016) : . 311 011 ICC; tested species: human
Cardiac atrial appendage stem cells engraft and differentiate into cardiomyocytes in vivo: A new tool for cardiac repair after MI.
Fanton Y, Robic B, Rummens JL, Daniëls A, Windmolders S, Willems L, Jamaer L, Dubois J, Bijnens E, Heuts N, Notelaers K, et al.
International journal of cardiology (2015) 201: 10-9. 311 011 ICC
Fibroblast Growth Factors and Vascular Endothelial Growth Factor Promote Cardiac Reprogramming under Defined Conditions.
Yamakawa H, Muraoka N, Miyamoto K, Sadahiro T, Isomi M, Haginiwa S, Kojima H, Umei T, Akiyama M, Kuishi Y, Kurokawa J, et al.
Stem cell reports (2015) 56: 1128-1142. 311 011 ICC
Direct nkx2-5 transcriptional repression of isl1 controls cardiomyocyte subtype identity.
Dorn T, Goedel A, Lam JT, Haas J, Tian Q, Herrmann F, Bundschu K, Dobreva G, Schiemann M, Dirschinger R, Guo Y, et al.
Stem cells (Dayton, Ohio) (2015) 334: 1113-29. 311 011 ICC, IHC
Phasic modulation of Wnt signaling enhances cardiac differentiation in human pluripotent stem cells by recapitulating developmental ontogeny.
Mehta A, Ramachandra CJ, Sequiera GL, Sudibyo Y, Nandihalli M, Yong PJ, Koh CH, Shim W
Biochimica et biophysica acta (2014) 184311: 2394-402. 311 011 ICC
Chemically defined generation of human cardiomyocytes.
Burridge PW, Matsa E, Shukla P, Lin ZC, Churko JM, Ebert AD, Lan F, Diecke S, Huber B, Mordwinkin NM, Plews JR, et al.
Nature methods (2014) 118: 855-60. 311 011 ICC
Simultaneous voltage and calcium mapping of genetically purified human induced pluripotent stem cell-derived cardiac myocyte monolayers.
Lee P, Klos M, Bollensdorff C, Hou L, Ewart P, Kamp TJ, Zhang J, Bizy A, Guerrero-Serna G, Kohl P, Jalife J, et al.
Circulation research (2012) 11012: 1556-63. 311 011 ICC, FACS
Dantrolene rescues arrhythmogenic RYR2 defect in a patient-specific stem cell model of catecholaminergic polymorphic ventricular tachycardia.
Jung CB, Moretti A, Mederos y Schnitzler M, Iop L, Storch U, Bellin M, Dorn T, Ruppenthal S, Pfeiffer S, Goedel A, Dirschinger RJ, et al.
EMBO molecular medicine (2012) 43: 180-91. 311 011 ICC; tested species: human
Tbx5 overexpression favors a first heart field lineage in murine embryonic stem cells and in Xenopus laevis embryos.
Herrmann F, Bundschu K, Kühl SJ, Kühl M
Developmental dynamics : an official publication of the American Association of Anatomists (2011) 24012: 2634-45. 311 011 ICC
NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes.
Elliott DA, Braam SR, Koutsis K, Ng ES, Jenny R, Lagerqvist EL, Biben C, Hatzistavrou T, Hirst CE, Yu QC, Skelton RJ, et al.
Nature methods (2011) 812: 1037-40. 311 011 ICC
Functional cardiomyocytes derived from human induced pluripotent stem cells.
Zhang J, Wilson GF, Soerens AG, Koonce CH, Yu J, Palecek SP, Thomson JA, Kamp TJ
Circulation research (2009) 1044: e30-41. 311 011 ICC
Cardiomyocyte enrichment from human embryonic stem cell cultures by selection of ALCAM surface expression.
Rust W, Balakrishnan T, Zweigerdt R
Regenerative medicine (2009) 42: 225-37. 311 011 ICC
Highly enriched cardiomyocytes from human embryonic stem cells.
Xu XQ, Zweigerdt R, Soo SY, Ngoh ZX, Tham SC, Wang ST, Graichen R, Davidson B, Colman A, Sun W
Cytotherapy (2008) 104: 376-89. 311 011 ICC, IHC
Cardiomyocytes from human and mouse embryonic stem cells.
Mummery C, van der Heyden MA, de Boer TP, Passier R, Ward D, van den Brink S, van Rooijen M, van de Stolpe A
Methods in molecular medicine (2007) 140: 249-72. 311 011 ICC; tested species: human,mouse
Development of In Vitro Drug-Induced Cardiotoxicity Assay by Using Three-Dimensional Cardiac Tissues Derived from Human Induced Pluripotent Stem Cells.
Takeda M, Miyagawa S, Fukushima S, Saito A, Ito E, Harada A, Matsuura R, Iseoka H, Sougawa N, Mochizuki-Oda N, Matsusaki M, et al.
Tissue engineering. Part C, Methods (2018) 241: 56-67. 311 011 IHC; tested species: stem cells
Human Embryonic Stem Cell-Derived Cardiomyocytes Self-Arrange with Areas of Different Subtypes During Differentiation.
Vestergaard ML, Grubb S, Koefoed K, Anderson-Jenkins Z, Grunnet-Lauridsen K, Calloe K, Clausen C, Christensen ST, Møllgård K, Andersen CY
Stem cells and development (2017) 2621: 1566-1577. 311 011 IHC; tested species: human
Direct nkx2-5 transcriptional repression of isl1 controls cardiomyocyte subtype identity.
Dorn T, Goedel A, Lam JT, Haas J, Tian Q, Herrmann F, Bundschu K, Dobreva G, Schiemann M, Dirschinger R, Guo Y, et al.
Stem cells (Dayton, Ohio) (2015) 334: 1113-29. 311 011 ICC, IHC
Human platelet lysate as a fetal bovine serum substitute improves human adipose-derived stromal cell culture for future cardiac repair applications.
Naaijkens BA, Niessen HW, Prins HJ, Krijnen PA, Kokhuis TJ, de Jong N, van Hinsbergh VW, Kamp O, Helder MN, Musters RJ, van Dijk A, et al.
Cell and tissue research (2012) 3481: 119-30. 311 011 IHC
Human cardiomyocyte progenitor cell transplantation preserves long-term function of the infarcted mouse myocardium.
Smits AM, van Laake LW, den Ouden K, Schreurs C, Szuhai K, van Echteld CJ, Mummery CL, Doevendans PA, Goumans MJ
Cardiovascular research (2009) 833: 527-35. 311 011 IHC
Highly enriched cardiomyocytes from human embryonic stem cells.
Xu XQ, Zweigerdt R, Soo SY, Ngoh ZX, Tham SC, Wang ST, Graichen R, Davidson B, Colman A, Sun W
Cytotherapy (2008) 104: 376-89. 311 011 ICC, IHC
Attachment and growth of human embryonic stem cells on microcarriers.
Phillips BW, Horne R, Lay TS, Rust WL, Teck TT, Crook JM
Journal of biotechnology (2008) 1381-2: 24-32. 311 011 IHC
Efficient expansion of clinical-grade human fibroblasts on microcarriers: cells suitable for ex vivo expansion of clinical-grade hESCs.
Phillips BW, Lim RY, Tan TT, Rust WL, Crook JM
Journal of biotechnology (2008) 1341-2: 79-87. 311 011 IHC
Human embryonic stem cell-derived cardiomyocytes survive and mature in the mouse heart and transiently improve function after myocardial infarction.
van Laake LW, Passier R, Monshouwer-Kloots J, Verkleij AJ, Lips DJ, Freund C, den Ouden K, Ward-van Oostwaard D, Korving J, Tertoolen LG, van Echteld CJ, et al.
Stem cell research (2007) 11: 9-24. 311 011 IHC
Survival and maturation of human embryonic stem cell-derived cardiomyocytes in rat hearts.
Dai W, Field LJ, Rubart M, Reuter S, Hale SL, Zweigerdt R, Graichen RE, Kay GL, Jyrala AJ, Colman A, Davidson BP, et al.
Journal of molecular and cellular cardiology (2007) 434: 504-16. 311 011 IHC
Phosphorylation and translocation of heat shock protein 27 and alphaB-crystallin in human myocardium after cardioplegia and cardiopulmonary bypass.
Clements RT, Sodha NR, Feng J, Mieno S, Boodhwani M, Ramlawi B, Bianchi C, Sellke FW
The Journal of thoracic and cardiovascular surgery (2007) 1346: 1461-70. 311 011 WB, IHC; tested species: human
Differential Expression Levels of Integrin α6 Enable the Selective Identification and Isolation of Atrial and Ventricular Cardiomyocytes.
Wiencierz AM, Kernbach M, Ecklebe J, Monnerat G, Tomiuk S, Raulf A, Christalla P, Malan D, Hesse M, Bosio A, Fleischmann BK, et al.
PloS one (2015) 1011: e0143538. 311 011 FACS
Induced pluripotent stem cells as a new strategy for cardiac regeneration and disease modeling.
Iglesias-García O, Pelacho B, Prósper F
Journal of molecular and cellular cardiology (2013) 62: 43-50. 311 011 FACS; tested species: human
Simultaneous voltage and calcium mapping of genetically purified human induced pluripotent stem cell-derived cardiac myocyte monolayers.
Lee P, Klos M, Bollensdorff C, Hou L, Ewart P, Kamp TJ, Zhang J, Bizy A, Guerrero-Serna G, Kohl P, Jalife J, et al.
Circulation research (2012) 11012: 1556-63. 311 011 ICC, FACS
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com