IP3-receptor type 1 - 117 003
Triggers release of Ca2+ from intracellular stores
Polyclonal rabbit purified antibody
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|Cat. No. 117 003||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) (see remarks) gallery
IP: not tested yet
ICC: not tested yet
IHC: 1 : 200 up to 1 : 500 gallery
IHC-P/FFPE: 1 : 200 gallery
|Immunogen||Synthetic peptide corresponding to AA 2731 to 2749 from rat IP3-receptortype1 (UniProt Id: P29994)|
Reacts with: human (Q14643), rat (P29994), mouse (P11881), cow.
Other species not tested yet.
|Specificity||Specific for InsP3.|
|matching control protein/peptide||117-0P|
WB: Due to its large size, IP3 receptor requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.