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Home > Products > 272 003

Glycine transporter2 antibody - 272 003 K.D.

Glycine transporters are transmembrane proteins involved in the removal of extracellular glycine
Rabbit polyclonal purified antibody
Cat. No.: 272 003
Amount: 50 µg
Price: $380.00
Cat. No. 272 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: yes gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 500 up to 1 : 1000 gallery  
Clarity: yes
Immunogen Recombinant protein corresponding to residues near the amino-terminus of rat Glycine transporter2. (UniProt Id: P58295)
Reactivity Reacts with: rat (P58295), mouse (Q761V0).
Other species not tested yet.
Specificity K.D. validated PubMed: 30881475
Matching control protein/peptide 272-0P
Remarks

WB:  Glycine transporter 2 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet 272_003.pdf

References for Glycine transporter2 - 272 003

Spinal Cord Glycine Transporter 2 Mediates Bilateral ST35 Acupoints Sensitization in Rats with Knee Osteoarthritis.
Bai F, Ma Y, Guo H, Li Y, Xu F, Zhang M, Dong H, Deng J, Xiong L
Evidence-based complementary and alternative medicine : eCAM (2019) 2019: 7493286. 272 003 WB; KD verified; tested species: rat
Sequential maturation of stimulus-specific adaptation in the mouse lemniscal auditory system.
Valerio P, Rechenmann J, Joshi S, De Franceschi G, Barkat TR
Science advances (2024) 101: eadi7624. 272 003 IHC; tested species: mouse
Kv3 channels contribute to the excitability of sub-populations of spinal cord neurons in lamina VII.
Mullen P, Pilati N, Large CH, Deuchars J, Deuchars S
eNeuro (2022) : . 272 003 IHC; tested species: mouse
Structural arrangement of auditory brainstem nuclei in the bats Phyllostomus discolor and Carollia perspicillata.
Pätz C, Console-Meyer L, Felmy F
The Journal of comparative neurology (2022) : . 272 003 IHC
CX3CR1 mutation alters synaptic and astrocytic protein expression, topographic gradients, and response latencies in the auditory brainstem.
Milinkeviciute G, Chokr SM, Castro EM, Cramer KS
The Journal of comparative neurology (2021) 52911: 3076-3097. 272 003 IHC; tested species: mouse
In vivo reprogramming of NG2 glia enables adult neurogenesis and functional recovery following spinal cord injury.
Tai W, Wu W, Wang LL, Ni H, Chen C, Yang J, Zang T, Zou Y, Xu XM, Zhang CL
Cell stem cell (2021) 285: 923-937.e4. 272 003 IHC; tested species: mouse
Arrangement of excitatory synaptic inputs on dendrites of the medial superior olive.
Callan AR, Heß M, Felmy F, Leibold C
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 272 003 IHC
Slow NMDA-mediated excitation accelerates offset-response latencies generated via a post-inhibitory rebound mechanism.
Rajaram E, Kaltenbach C, Fischl MJ, Mrowka L, Alexandrova O, Grothe B, Hennig MH, Kopp-Scheinpflug C
eNeuro (2019) : . 272 003 IHC; tested species: mouse
Hyper-formation of GABA and glycine co-releasing terminals in the mouse cerebellar nuclei after deprivation of GABAergic inputs from Purkinje cells.
Kobayashi S, Kim J, Yanagawa Y, Suzuki N, Saito H, Takayama C
Neuroscience (2019) : . 272 003 IHC, EM; tested species: mouse
Distinct Distribution Patterns of Potassium Channel Sub-Units in Somato-Dendritic Compartments of Neurons of the Medial Superior Olive.
Nabel AL, Callan AR, Gleiss SA, Kladisios N, Leibold C, Felmy F
Frontiers in cellular neuroscience (2019) 13: 38. 272 003 IHC
Tonotopic alterations in inhibitory input to the medial nucleus of the trapezoid body in a mouse model of Fragile X syndrome.
McCullagh EA, Salcedo E, Huntsman MM, Klug A
The Journal of comparative neurology (2017) 52516: 3543-3562. 272 003 IHC; tested species: mouse
Physiology and anatomy of neurons in the medial superior olive of the mouse.
Fischl MJ, Burger RM, Schmidt-Pauly M, Alexandrova O, Sinclair JL, Grothe B, Forsythe ID, Kopp-Scheinpflug C
Journal of neurophysiology (2016) 1166: 2676-2688. 272 003 IHC; tested species: mouse
Aging alters mechanisms underlying voluntary movements in spinal motor neurons of mice, primates, and humans.
Castro RW, Lopes MC, Settlage RE, Valdez G
JCI insight (2023) 89: . 272 003 Clarity; tested species: mouse
Hyper-formation of GABA and glycine co-releasing terminals in the mouse cerebellar nuclei after deprivation of GABAergic inputs from Purkinje cells.
Kobayashi S, Kim J, Yanagawa Y, Suzuki N, Saito H, Takayama C
Neuroscience (2019) : . 272 003 IHC, EM; tested species: mouse
Cat. No.: 272 003
Amount: 50 µg
Price: $380.00
Spinal Cord Glycine Transporter 2 Mediates Bilateral ST35 Acupoints Sensitization in Rats with Knee Osteoarthritis.
Bai F, Ma Y, Guo H, Li Y, Xu F, Zhang M, Dong H, Deng J, Xiong L
Evidence-based complementary and alternative medicine : eCAM (2019) 2019: 7493286. 272 003 WB; KD verified; tested species: rat
Sequential maturation of stimulus-specific adaptation in the mouse lemniscal auditory system.
Valerio P, Rechenmann J, Joshi S, De Franceschi G, Barkat TR
Science advances (2024) 101: eadi7624. 272 003 IHC; tested species: mouse
Kv3 channels contribute to the excitability of sub-populations of spinal cord neurons in lamina VII.
Mullen P, Pilati N, Large CH, Deuchars J, Deuchars S
eNeuro (2022) : . 272 003 IHC; tested species: mouse
Structural arrangement of auditory brainstem nuclei in the bats Phyllostomus discolor and Carollia perspicillata.
Pätz C, Console-Meyer L, Felmy F
The Journal of comparative neurology (2022) : . 272 003 IHC
CX3CR1 mutation alters synaptic and astrocytic protein expression, topographic gradients, and response latencies in the auditory brainstem.
Milinkeviciute G, Chokr SM, Castro EM, Cramer KS
The Journal of comparative neurology (2021) 52911: 3076-3097. 272 003 IHC; tested species: mouse
In vivo reprogramming of NG2 glia enables adult neurogenesis and functional recovery following spinal cord injury.
Tai W, Wu W, Wang LL, Ni H, Chen C, Yang J, Zang T, Zou Y, Xu XM, Zhang CL
Cell stem cell (2021) 285: 923-937.e4. 272 003 IHC; tested species: mouse
Arrangement of excitatory synaptic inputs on dendrites of the medial superior olive.
Callan AR, Heß M, Felmy F, Leibold C
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 272 003 IHC
Slow NMDA-mediated excitation accelerates offset-response latencies generated via a post-inhibitory rebound mechanism.
Rajaram E, Kaltenbach C, Fischl MJ, Mrowka L, Alexandrova O, Grothe B, Hennig MH, Kopp-Scheinpflug C
eNeuro (2019) : . 272 003 IHC; tested species: mouse
Hyper-formation of GABA and glycine co-releasing terminals in the mouse cerebellar nuclei after deprivation of GABAergic inputs from Purkinje cells.
Kobayashi S, Kim J, Yanagawa Y, Suzuki N, Saito H, Takayama C
Neuroscience (2019) : . 272 003 IHC, EM; tested species: mouse
Distinct Distribution Patterns of Potassium Channel Sub-Units in Somato-Dendritic Compartments of Neurons of the Medial Superior Olive.
Nabel AL, Callan AR, Gleiss SA, Kladisios N, Leibold C, Felmy F
Frontiers in cellular neuroscience (2019) 13: 38. 272 003 IHC
Tonotopic alterations in inhibitory input to the medial nucleus of the trapezoid body in a mouse model of Fragile X syndrome.
McCullagh EA, Salcedo E, Huntsman MM, Klug A
The Journal of comparative neurology (2017) 52516: 3543-3562. 272 003 IHC; tested species: mouse
Physiology and anatomy of neurons in the medial superior olive of the mouse.
Fischl MJ, Burger RM, Schmidt-Pauly M, Alexandrova O, Sinclair JL, Grothe B, Forsythe ID, Kopp-Scheinpflug C
Journal of neurophysiology (2016) 1166: 2676-2688. 272 003 IHC; tested species: mouse
Aging alters mechanisms underlying voluntary movements in spinal motor neurons of mice, primates, and humans.
Castro RW, Lopes MC, Settlage RE, Valdez G
JCI insight (2023) 89: . 272 003 Clarity; tested species: mouse
Hyper-formation of GABA and glycine co-releasing terminals in the mouse cerebellar nuclei after deprivation of GABAergic inputs from Purkinje cells.
Kobayashi S, Kim J, Yanagawa Y, Suzuki N, Saito H, Takayama C
Neuroscience (2019) : . 272 003 IHC, EM; tested species: mouse
WB
ICC
IHC
IHC
IHC-P
IHC-P
Background
Glycine is the major inhibitory neurotransmitter in the spinal cord and brainstem. Two differentially expressed glycine transporters, GLYT 1 and GLYT 2, regulate the extracellular concentration of this neuroactive amino acid in the synaptic cleft.
GLYT 1 is expressed in both neurons as well as in glia with high expression levels in the main olfactory bulb, cerebellum, brainstem and spinal cord and low expression in other brain regions. It has been hypothesized to regulate glycine levels in NMDA receptor-mediated neurotransmission.
GLYT 2 shows an axonal localization and is mainly expressed in spinal cord, brain-stem and cerebellum.
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