GFP - 132 003CpH
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Polyclonal rabbit purified antibody
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|Cat. No. 132 003CpH||
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with CypHer5E. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
ICC: 1 : 100 up to 1 : 1000 gallery
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Immunogen||Recombinant protein corresponding to AA 1 to 238 from GFP (UniProt Id: P42212)|
|Specificity||Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants.Does not cross-react to mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.|
|Remarks||This antibody is suitable for internalization studies when GFP is fused to an epitope residing in the lumen of an acidic compartment.|