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Gephyrin - 147 111

A protein associated with inhibitory neurotransmitter receptors

Monoclonal mouse purified IgG
415.00 USD
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Cat. No. 147 111 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 5000 gallery  
IP: yes
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: yes , methanol-acetone fixation
IHC-P/FFPE: not recommended
ELISA: yes
Clone 3B11
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 307 to 735 from rat Gephyrin (UniProt Id: Q03555)
Epitop Epitop: AA 326 to 550 from rat Gephyrin (UniProt Id: Q03555)
Reactivity Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), zebrafish.
Other species not tested yet.
Specificity Detects all splice variants that contain a complete E-domain including the C6 domain. (K.O. verified)
Remarks This antibody is highly recommended for Western blot experiments and immunoprecipitation.
Datasheet 147_111.pdf


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References for Gephyrin - 147 111

Developmental changes in plasticity, synaptic, glia, and connectivity protein levels in rat basolateral amygdala.
Bessières B, Jia M, Travaglia A, Alberini CM
Learning & memory (Cold Spring Harbor, N.Y.) (2019) 2611: 436-448. 147 111 WB; tested species: rat
SETD5 Regulates Chromatin Methylation State and Preserves Global Transcriptional Fidelity during Brain Development and Neuronal Wiring.
Sessa A, Fagnocchi L, Mastrototaro G, Massimino L, Zaghi M, Indrigo M, Cattaneo S, Martini D, Gabellini C, Pucci C, Fasciani A, et al.
Neuron (2019) : . 147 111 WB; tested species: mouse
CYP46A1 Activation by Efavirenz Leads to Behavioral Improvement without Significant Changes in Amyloid Plaque Load in the Brain of 5XFAD Mice.
Petrov AM, Lam M, Mast N, Moon J, Li Y, Maxfield E, Pikuleva IA
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics (2019) : . 147 111 WB; tested species: mouse
Lrfn2-Mutant Mice Display Suppressed Synaptic Plasticity and Inhibitory Synapse Development and Abnormal Social Communication and Startle Response.
Li Y, Kim R, Cho YS, Song WS, Kim D, Kim K, Roh JD, Chung C, Park H, Yang E, Kim SJ, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) 3826: 5872-5887. 147 111 WB; tested species: mouse
Developmental changes in plasticity, synaptic, glia, and connectivity protein levels in rat medial prefrontal cortex.
Jia M, Travaglia A, Pollonini G, Fedele G, Alberini CM
Learning & memory (Cold Spring Harbor, N.Y.) (2018) 2510: 533-543. 147 111 WB; tested species: rat
UPR activation specifically modulates glutamate neurotransmission in the cerebellum of a mouse model of autism.
Trobiani L, Favaloro FL, Di Castro MA, Di Mattia M, Cariello M, Miranda E, Canterini S, De Stefano ME, Comoletti D, Limatola C, De Jaco A, et al.
Neurobiology of disease (2018) : . 147 111 WB; tested species: mouse
Reciprocal control of excitatory synapse numbers by Wnt and Wnt inhibitor PRR7 secreted on exosomes.
Lee SH, Shin SM, Zhong P, Kim HT, Kim DI, Kim JM, Do Heo W, Kim DW, Yeo CY, Kim CH, Liu QS, et al.
Nature communications (2018) 91: 3434. 147 111 WB; tested species: rat
Neuroligin 1, 2, and 3 Regulation at the Synapse: FMRP-Dependent Translation and Activity-Induced Proteolytic Cleavage.
Chmielewska JJ, Kuzniewska B, Milek J, Urbanska K, Dziembowska M
Molecular neurobiology (2018) : . 147 111 WB; tested species: mouse
Estradiol modulates the efficacy of synaptic inhibition by decreasing the dwell time of GABAA receptors at inhibitory synapses.
Mukherjee J, Cardarelli RA, Cantaut-Belarif Y, Deeb TZ, Srivastava DP, Tyagarajan SK, Pangalos MN, Triller A, Maguire J, Brandon NJ, Moss SJ, et al.
Proceedings of the National Academy of Sciences of the United States of America (2017) 11444: 11763-11768. 147 111 WB, ICC
Cadherin-10 Maintains Excitatory/Inhibitory Ratio through Interactions with Synaptic Proteins.
Smith KR, Jones KA, Kopeikina KJ, Burette AC, Copits BA, Yoon S, Forrest MP, Fawcett-Patel JM, Hanley JG, Weinberg RJ, Swanson GT, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 3746: 11127-11139. 147 111 WB; tested species: rat
Analysis of SUMO1-conjugation at synapses.
Daniel JA, Cooper BH, Palvimo JJ, Zhang FP, Brose N, Tirard M
eLife (2017) 6: . 147 111 WB; tested species: mouse
S-sulfocysteine/NMDA receptor-dependent signaling underlies neurodegeneration in molybdenum cofactor deficiency.
Kumar A, Dejanovic B, Hetsch F, Semtner M, Fusca D, Arjune S, Santamaria-Araujo JA, Winkelmann A, Ayton S, Bush AI, Kloppenburg P, et al.
The Journal of clinical investigation (2017) 12712: 4365-4378. 147 111 WB, ICC; tested species: mouse
IgSF21 promotes differentiation of inhibitory synapses via binding to neurexin2α.
Tanabe Y, Naito Y, Vasuta C, Lee AK, Soumounou Y, Linhoff MW, Takahashi H
Nature communications (2017) 81: 408. 147 111 WB
Artemisinins Target GABAA Receptor Signaling and Impair α Cell Identity.
Li J, Casteels T, Frogne T, Ingvorsen C, Honoré C, Courtney M, Huber KVM, Schmitner N, Kimmel RA, Romanov RA, Sturtzel C, et al.
Cell (2017) 1681-2: 86-100.e15. 147 111 WB
Forebrain-specific loss of synaptic GABAA receptors results in altered neuronal excitability and synaptic plasticity in mice.
O'Sullivan GA, Jedlicka P, Chen HX, Kalbouneh H, Ippolito A, Deller T, Nawrotzki RA, Kuhse J, Kalaidzidis YL, Kirsch J, Schwarzacher SW, et al.
Molecular and cellular neurosciences (2016) 72: 101-13. 147 111 WB; KO verified
How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 147 111 WB
Several posttranslational modifications act in concert to regulate gephyrin scaffolding and GABAergic transmission.
Ghosh H, Auguadri L, Battaglia S, Simone Thirouin Z, Zemoura K, Messner S, Acuña MA, Wildner H, Yévenes GE, Dieter A, Kawasaki H, et al.
Nature communications (2016) 7: 13365. 147 111 WB, IP
Proteomic Characterization of Inhibitory Synapses Using a Novel pHluorin-tagged γ-Aminobutyric Acid Receptor, Type A (GABAA), α2 Subunit Knock-in Mouse.
Nakamura Y, Morrow DH, Modgil A, Huyghe D, Deeb TZ, Lumb MJ, Davies PA, Moss SJ
The Journal of biological chemistry (2016) 29123: 12394-407. 147 111 WB
Synaptic localization of α5 GABA (A) receptors via gephyrin interaction regulates dendritic outgrowth and spine maturation.
Brady ML, Jacob TC
Developmental neurobiology (2015) 7511: 1241-51. 147 111 WB, ICC; tested species: rat
Activity-dependent inhibitory synapse remodeling through gephyrin phosphorylation.
Flores CE, Nikonenko I, Mendez P, Fritschy JM, Tyagarajan SK, Muller D
Proceedings of the National Academy of Sciences of the United States of America (2015) 1121: E65-72. 147 111 WB
Neuroligin-1 regulates excitatory synaptic transmission, LTP and EPSP-spike coupling in the dentate gyrus in vivo.
Jedlicka P, Vnencak M, Krueger DD, Jungenitz T, Brose N, Schwarzacher SW
Brain structure & function (2015) 2201: 47-58. 147 111 WB
Lipid binding defects and perturbed synaptogenic activity of a Collybistin R290H mutant that causes epilepsy and intellectual disability.
Papadopoulos T, Schemm R, Grubmüller H, Brose N
The Journal of biological chemistry (2015) 29013: 8256-70. 147 111 WB
Neuroligin1 drives synaptic and behavioral maturation through intracellular interactions.
Hoy JL, Haeger PA, Constable JR, Arias RJ, McCallum R, Kyweriga M, Davis L, Schnell E, Wehr M, Castillo PE, Washbourne P, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3322: 9364-84. 147 111 WB; tested species: mouse
Extracellular signal-regulated kinase and glycogen synthase kinase 3β regulate gephyrin postsynaptic aggregation and GABAergic synaptic function in a calpain-dependent mechanism.
Tyagarajan SK, Ghosh H, Yévenes GE, Imanishi SY, Zeilhofer HU, Gerrits B, Fritschy JM
The Journal of biological chemistry (2013) 28814: 9634-47. 147 111 WB, IP
Target-derived matricryptins organize cerebellar synapse formation through α3β1 integrins.
Su J, Stenbjorn RS, Gorse K, Su K, Hauser KF, Ricard-Blum S, Pihlajaniemi T, Fox MA
Cell reports (2012) 22: 223-30. 147 111 WB; tested species: mouse
Duplicated gephyrin genes showing distinct tissue distribution and alternative splicing patterns mediate molybdenum cofactor biosynthesis, glycine receptor clustering, and escape behavior in zebrafish.
Ogino K, Ramsden SL, Keib N, Schwarz G, Harvey RJ, Hirata H
The Journal of biological chemistry (2011) 2861: 806-17. 147 111 WB; tested species: zebrafish
Neuroligin-4 is localized to glycinergic postsynapses and regulates inhibition in the retina.
Hoon M, Soykan T, Falkenburger B, Hammer M, Patrizi A, Schmidt KF, Sassoè-Pognetto M, Löwel S, Moser T, Taschenberger H, Brose N, et al.
Proceedings of the National Academy of Sciences of the United States of America (2011) 1087: 3053-8. 147 111 WB, IHC
Gephyrin regulates GABAergic and glutamatergic synaptic transmission in hippocampal cell cultures.
Varley ZK, Pizzarelli R, Antonelli R, Stancheva SH, Kneussel M, Cherubini E, Zacchi P
The Journal of biological chemistry (2011) 28623: 20942-51. 147 111 WB
Irregular RNA splicing curtails postsynaptic gephyrin in the cornu ammonis of patients with epilepsy.
Förstera B, Belaidi AA, Jüttner R, Bernert C, Tsokos M, Lehmann TN, Horn P, Dehnicke C, Schwarz G, Meier JC
Brain : a journal of neurology (2010) 133Pt 12: 3778-94. 147 111 WB
Differential localization of gamma-aminobutyric acid type A and glycine receptor subunits and gephyrin in the human pons, medulla oblongata and uppermost cervical segment of the spinal cord: an immunohistochemical study.
Waldvogel HJ, Baer K, Eady E, Allen KL, Gilbert RT, Mohler H, Rees MI, Nicholson LF, Faull RL
The Journal of comparative neurology (2010) 5183: 305-28. 147 111 WB, IHC; tested species: human
Alpha-synuclein promotes SNARE-complex assembly in vivo and in vitro.
Burré J, Sharma M, Tsetsenis T, Buchman V, Etherton MR, Südhof TC
Science (New York, N.Y.) (2010) 3295999: 1663-7. 147 111 WB
Splice-specific functions of gephyrin in molybdenum cofactor biosynthesis.
Smolinsky B, Eichler SA, Buchmeier S, Meier JC, Schwarz G
The Journal of biological chemistry (2008) 28325: 17370-9. 147 111 WB
Chronic inflammatory pain induced GABAergic synaptic plasticity in the adult mouse anterior cingulate cortex.
Koga K, Shimoyama S, Yamada A, Furukawa T, Nikaido Y, Furue H, Nakamura K, Ueno S
Molecular pain () 14: 1744806918783478. 147 111 WB; tested species: mouse
Changes in the Synaptic Proteome in Tauopathy and Rescue of Tau-Induced Synapse Loss by C1q Antibodies.
Dejanovic B, Huntley MA, De Mazière A, Meilandt WJ, Wu T, Srinivasan K, Jiang Z, Gandham V, Friedman BA, Ngu H, Foreman O, et al.
Neuron (2018) : . 147 111 IP; tested species: mouse
Several posttranslational modifications act in concert to regulate gephyrin scaffolding and GABAergic transmission.
Ghosh H, Auguadri L, Battaglia S, Simone Thirouin Z, Zemoura K, Messner S, Acuña MA, Wildner H, Yévenes GE, Dieter A, Kawasaki H, et al.
Nature communications (2016) 7: 13365. 147 111 WB, IP
Extracellular signal-regulated kinase and glycogen synthase kinase 3β regulate gephyrin postsynaptic aggregation and GABAergic synaptic function in a calpain-dependent mechanism.
Tyagarajan SK, Ghosh H, Yévenes GE, Imanishi SY, Zeilhofer HU, Gerrits B, Fritschy JM
The Journal of biological chemistry (2013) 28814: 9634-47. 147 111 WB, IP
An Autaptic Culture System for Standardized Analyses of iPSC-Derived Human Neurons.
Rhee HJ, Shaib AH, Rehbach K, Lee C, Seif P, Thomas C, Gideons E, Guenther A, Krutenko T, Hebisch M, Peitz M, et al.
Cell reports (2019) 277: 2212-2228.e7. 147 111 ICC; tested species: human
The catalytic function of the gephyrin-binding protein IQSEC3 regulates neurotransmitter-specific matching of pre- and postsynaptic structures in primary hippocampal cultures.
Früh S, Tyagarajan SK, Campbell B, Bosshard G, Fritschy JM
Journal of neurochemistry (2018) : . 147 111 ICC; tested species: rat
A unique intracellular tyrosine in neuroligin-1 regulates AMPA receptor recruitment during synapse differentiation and potentiation.
Letellier M, Szíber Z, Chamma I, Saphy C, Papasideri I, Tessier B, Sainlos M, Czöndör K, Thoumine O
Nature communications (2018) 91: 3979. 147 111 ICC; tested species: rat
Estradiol modulates the efficacy of synaptic inhibition by decreasing the dwell time of GABAA receptors at inhibitory synapses.
Mukherjee J, Cardarelli RA, Cantaut-Belarif Y, Deeb TZ, Srivastava DP, Tyagarajan SK, Pangalos MN, Triller A, Maguire J, Brandon NJ, Moss SJ, et al.
Proceedings of the National Academy of Sciences of the United States of America (2017) 11444: 11763-11768. 147 111 WB, ICC
S-sulfocysteine/NMDA receptor-dependent signaling underlies neurodegeneration in molybdenum cofactor deficiency.
Kumar A, Dejanovic B, Hetsch F, Semtner M, Fusca D, Arjune S, Santamaria-Araujo JA, Winkelmann A, Ayton S, Bush AI, Kloppenburg P, et al.
The Journal of clinical investigation (2017) 12712: 4365-4378. 147 111 WB, ICC; tested species: mouse
Transient oxytocin signaling primes the development and function of excitatory hippocampal neurons.
Ripamonti S, Ambrozkiewicz MC, Guzzi F, Gravati M, Biella G, Bormuth I, Hammer M, Tuffy LP, Sigler A, Kawabe H, Nishimori K, et al.
eLife (2017) 6: . 147 111 ICC; tested species: mouse
Bidirectional Homeostatic Regulation of a Depression-Related Brain State by Gamma-Aminobutyric Acidergic Deficits and Ketamine Treatment.
Ren Z, Pribiag H, Jefferson SJ, Shorey M, Fuchs T, Stellwagen D, Luscher B
Biological psychiatry (2016) 806: 457-468. 147 111 ICC
Corticotropin-Releasing Hormone Receptor Type 1 (CRHR1) Clustering with MAGUKs Is Mediated via Its C-Terminal PDZ Binding Motif.
Bender J, Engeholm M, Ederer MS, Breu J, Møller TC, Michalakis S, Rasko T, Wanker EE, Biel M, Martinez KL, Wurst W, et al.
PloS one (2015) 109: e0136768. 147 111 ICC
Synaptic localization of α5 GABA (A) receptors via gephyrin interaction regulates dendritic outgrowth and spine maturation.
Brady ML, Jacob TC
Developmental neurobiology (2015) 7511: 1241-51. 147 111 WB, ICC; tested species: rat
Synaptic recruitment of gephyrin regulates surface GABAA receptor dynamics for the expression of inhibitory LTP.
Petrini EM, Ravasenga T, Hausrat TJ, Iurilli G, Olcese U, Racine V, Sibarita JB, Jacob TC, Moss SJ, Benfenati F, Medini P, et al.
Nature communications (2014) 5: 3921. 147 111 ICC; tested species: mouse
Neuronal nitric oxide synthase-dependent S-nitrosylation of gephyrin regulates gephyrin clustering at GABAergic synapses.
Dejanovic B, Schwarz G
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3423: 7763-8. 147 111 ICC
Neurobeachin regulates neurotransmitter receptor trafficking to synapses.
Nair R, Lauks J, Jung S, Cooke NE, de Wit H, Brose N, Kilimann MW, Verhage M, Rhee J
The Journal of cell biology (2013) 2001: 61-80. 147 111 ICC
Neuronal profilin isoforms are addressed by different signalling pathways.
Murk K, Wittenmayer N, Michaelsen-Preusse K, Dresbach T, Schoenenberger CA, Korte M, Jockusch BM, Rothkegel M
PloS one (2012) 73: e34167. 147 111 ICC; tested species: mouse,rat
Activity-dependent reconnection of adult-born dentate granule cells in a mouse model of frontotemporal dementia.
Terreros-Roncal J, Flor-García M, Moreno-Jiménez EP, Pallas-Bazarra N, Rábano A, Sah N, van Praag H, Giacomini D, Schinder AF, Ávila J, Llorens-Martín M, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 147 111 IHC; tested species: mouse
Loss of Neuroligin3 specifically downregulates retinal GABAAα2 receptors without abolishing direction selectivity.
Hoon M, Krishnamoorthy V, Gollisch T, Falkenburger B, Varoqueaux F
PloS one (2017) 127: e0181011. 147 111 IHC; tested species: mouse
Genetic Deletion of the Clathrin Adaptor GGA3 Reduces Anxiety and Alters GABAergic Transmission.
Walker KR, Modgil A, Albrecht D, Lomoio S, Haydon PG, Moss SJ, Tesco G
PloS one (2016) 115: e0155799. 147 111 IHC
FMRP regulates multipolar to bipolar transition affecting neuronal migration and cortical circuitry.
La Fata G, Gärtner A, Domínguez-Iturza N, Dresselaers T, Dawitz J, Poorthuis RB, Averna M, Himmelreich U, Meredith RM, Achsel T, Dotti CG, et al.
Nature neuroscience (2014) 1712: 1693-700. 147 111 IHC; tested species: mouse
Developmental regulation and activity-dependent maintenance of GABAergic presynaptic inhibition onto rod bipolar cell axonal terminals.
Schubert T, Hoon M, Euler T, Lukasiewicz PD, Wong RO
Neuron (2013) 781: 124-37. 147 111 IHC; tested species: mouse
Neuroligin-4 is localized to glycinergic postsynapses and regulates inhibition in the retina.
Hoon M, Soykan T, Falkenburger B, Hammer M, Patrizi A, Schmidt KF, Sassoè-Pognetto M, Löwel S, Moser T, Taschenberger H, Brose N, et al.
Proceedings of the National Academy of Sciences of the United States of America (2011) 1087: 3053-8. 147 111 WB, IHC
Differential localization of gamma-aminobutyric acid type A and glycine receptor subunits and gephyrin in the human pons, medulla oblongata and uppermost cervical segment of the spinal cord: an immunohistochemical study.
Waldvogel HJ, Baer K, Eady E, Allen KL, Gilbert RT, Mohler H, Rees MI, Nicholson LF, Faull RL
The Journal of comparative neurology (2010) 5183: 305-28. 147 111 WB, IHC; tested species: human
Localization of glycine receptors in the human forebrain, brainstem, and cervical spinal cord: an immunohistochemical review.
Baer K, Waldvogel HJ, Faull RL, Rees MI
Frontiers in molecular neuroscience (2009) 2: 25. 147 111 IHC
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com