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SY SY - Synaptic Systems SY SY - Synaptic Systems

Gephyrin - 147 021

A protein associated with inhibitory neurotransmitter receptors

Monoclonal mouse hybridoma supernatant
415.00 USD
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Cat. No. 147 021 300 µl hybridoma supernatant, lyophilized. For reconstitution add 300 µl H2O, then aliquot and store at -20°C until use.
Applications WB: not recommended (see remarks)
IP: not recommended (see remarks)
ICC: 1 : 250 up to 1 : 500 gallery  
IHC: yes (see remarks) gallery  
IHC-P/FFPE: not tested yet
EM: yes
Clone mAb7a
Subtype IgG1 (κ light chain)
Immunogen Native Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop Epitop: AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reactivity Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish, frog.
Other species not tested yet.
Specificity Specific for the brain specific 93 kDa splice variant. (K.O. verified)
Remarks

WB: Clone 3B11 (cat. no. 147 111) highly recommended.

IP: Clone 3B11 (cat. no. 147 111) highly recommended.

IHC: Hybridoma supernatant highly recommended. For best results use the protocol of Schneider Gasser et al., 2006.
Alternatively AGR (10 mM citrate, pH 6.0, over night at 60°C) can be applied.

Datasheet 147_021.pdf


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References for Gephyrin - 147 021

Expression of Neurofilament Subunits at Neocortical Glutamatergic and GABAergic Synapses.
Bragina L, Conti F
Frontiers in neuroanatomy (2018) 12: 74. 147 021 WB, IHC; tested species: rat
Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.
Li J, Han W, Pelkey KA, Duan J, Mao X, Wang YX, Craig MT, Dong L, Petralia RS, McBain CJ, Lu W, et al.
Neuron (2017) 964: 808-826.e8. 147 021 WB, ICC, IHC; tested species: mouse
Sorting nexin 27 rescues neuroligin 2 from lysosomal degradation to control inhibitory synapse number.
Binda C, Nakamura Y, Henley J, Wilkinson K
The Biochemical journal (2019) : . 147 021 ICC; tested species: rat
Heparan Sulfate Organizes Neuronal Synapses through Neurexin Partnerships.
Zhang P, Lu H, Peixoto RT, Pines MK, Ge Y, Oku S, Siddiqui TJ, Xie Y, Wu W, Archer-Hartmann S, Yoshida K, et al.
Cell (2018) : . 147 021 ICC; tested species: rat
The Role of Agrin, Lrp4 and MuSK during Dendritic Arborization and Synaptogenesis in Cultured Embryonic CNS Neurons.
Handara G, Hetsch FJA, Jüttner R, Schick A, Haupt C, Rathjen FG, Kröger S
Developmental biology (2018) : . 147 021 ICC; tested species: mouse
Pentraxin 3 regulates synaptic function by inducing AMPA receptor clustering via ECM remodeling and β1-integrin.
Fossati G, Pozzi D, Canzi A, Mirabella F, Valentino S, Morini R, Ghirardini E, Filipello F, Moretti M, Gotti C, Annis DS, et al.
The EMBO journal (2018) : . 147 021 ICC; tested species: mouse
Nanoscale Structural Plasticity of the Active Zone Matrix Modulates Presynaptic Function.
Glebov OO, Jackson RE, Winterflood CM, Owen DM, Barker EA, Doherty P, Ewers H, Burrone J
Cell reports (2017) 1811: 2715-2728. 147 021 ICC
Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.
Li J, Han W, Pelkey KA, Duan J, Mao X, Wang YX, Craig MT, Dong L, Petralia RS, McBain CJ, Lu W, et al.
Neuron (2017) 964: 808-826.e8. 147 021 WB, ICC, IHC; tested species: mouse
Transient oxytocin signaling primes the development and function of excitatory hippocampal neurons.
Ripamonti S, Ambrozkiewicz MC, Guzzi F, Gravati M, Biella G, Bormuth I, Hammer M, Tuffy LP, Sigler A, Kawabe H, Nishimori K, et al.
eLife (2017) 6: . 147 021 ICC; tested species: mouse
Proteomic Characterization of Inhibitory Synapses Using a Novel pHluorin-tagged γ-Aminobutyric Acid Receptor, Type A (GABAA), α2 Subunit Knock-in Mouse.
Nakamura Y, Morrow DH, Modgil A, Huyghe D, Deeb TZ, Lumb MJ, Davies PA, Moss SJ
The Journal of biological chemistry (2016) 29123: 12394-407. 147 021 ICC
DISC1, astrocytes and neuronal maturation: a possible mechanistic link with implications for mental disorders.
Xia M, Zhu S, Shevelkin A, Ross CA, Pletnikov M
Journal of neurochemistry (2016) 1384: 518-24. 147 021 ICC; tested species: mouse
Electron tomography on γ-aminobutyric acid-ergic synapses reveals a discontinuous postsynaptic network of filaments.
Linsalata AE, Chen X, Winters CA, Reese TS
The Journal of comparative neurology (2014) 5224: 921-36. 147 021 ICC, EM; tested species: rat
Identification and characterization of GABA(A) receptor autoantibodies in autoimmune encephalitis.
Ohkawa T, Satake S, Yokoi N, Miyazaki Y, Ohshita T, Sobue G, Takashima H, Watanabe O, Fukata Y, Fukata M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3424: 8151-63. 147 021 ICC; tested species: rat
Molecular and functional interaction between protocadherin-γC5 and GABAA receptors.
Li Y, Xiao H, Chiou TT, Jin H, Bonhomme B, Miralles CP, Pinal N, Ali R, Chen WV, Maniatis T, De Blas AL, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3234: 11780-97. 147 021 ICC
A readily retrievable pool of synaptic vesicles.
Hua Y, Sinha R, Thiel CS, Schmidt R, Hüve J, Martens H, Hell SW, Egner A, Klingauf J
Nature neuroscience (2011) 147: 833-9. 147 021 ICC
Expression of protocadherin-γC4 protein in the rat brain.
Miralles CP, Taylor MJ, Bear J, Fekete CD, George S, Li Y, Bonhomme B, Chiou TT, De Blas AL
The Journal of comparative neurology (2019) : . 147 021 IHC; tested species: rat
Expression mapping, quantification, and complex formation of GluD1 and GluD2 glutamate receptors in adult mouse brain.
Nakamoto C, Konno K, Miyazaki T, Nakatsukasa E, Natsume R, Abe M, Kawamura M, Fukazawa Y, Shigemto R, Yamasaki M, Sakimura K, et al.
The Journal of comparative neurology (2019) : . 147 021 IHC; tested species: mouse
Cell type-specific distribution of GABAA receptor subtypes in the mouse dorsal striatum.
Boccalaro IL, Cristiá-Lara L, Schwerdel C, Fritschy JM, Rubi L
The Journal of comparative neurology (2019) : . 147 021 IHC; tested species: mouse
Subretinal Human Umbilical Tissue-Derived Cell Transplantation Preserves Retinal Synaptic Connectivity and Attenuates Müller Glial Reactivity.
Koh S, Chen WJ, Dejneka NS, Harris IR, Lu B, Girman S, Saylor J, Wang S, Eroglu C
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) 3812: 2923-2943. 147 021 IHC; tested species: rat
Expression of Neurofilament Subunits at Neocortical Glutamatergic and GABAergic Synapses.
Bragina L, Conti F
Frontiers in neuroanatomy (2018) 12: 74. 147 021 WB, IHC; tested species: rat
Extrasynaptic homomeric glycine receptors in neurons of the rat trigeminal mesencephalic nucleus.
Bae JY, Lee JS, Ko SJ, Cho YS, Rah JC, Cho HJ, Park MJ, Bae YC
Brain structure & function (2018) : . 147 021 IHC, EM; tested species: rat
Development of inhibitory synaptic inputs on layer 2/3 pyramidal neurons in the rat medial prefrontal cortex.
Virtanen MA, Lacoh CM, Fiumelli H, Kosel M, Tyagarajan S, de Roo M, Vutskits L
Brain structure & function (2018) 2234: 1999-2012. 147 021 IHC; tested species: rat
GABAergic malfunction in the anterior cingulate cortex underlying maternal immune activation-induced social deficits.
Okamoto K, Hitora-Imamura N, Hioki H, Ikegaya Y
Journal of neuroimmunology (2018) 321: 92-96. 147 021 IHC; tested species: mouse
Excitatory synaptic dysfunction cell-autonomously decreases inhibitory inputs and disrupts structural and functional plasticity.
He HY, Shen W, Zheng L, Guo X, Cline HT
Nature communications (2018) 91: 2893. 147 021 IHC; tested species: frog
Glycine receptors expression in rat spinal cord and dorsal root ganglion in prostaglandin E2 intrathecal injection models.
Wang HC, Cheng KI, Chen PR, Tseng KY, Kwan AL, Chang LL
BMC neuroscience (2018) 191: 72. 147 021 IHC; tested species: rat
The Cellular and Synaptic Architecture of the Mechanosensory Dorsal Horn.
Abraira VE, Kuehn ED, Chirila AM, Springel MW, Toliver AA, Zimmerman AL, Orefice LL, Boyle KA, Bai L, Song BJ, Bashista KA, et al.
Cell (2017) 1681-2: 295-310.e19. 147 021 IHC; tested species: mouse
In vivo transgenic expression of collybistin in neurons of the rat cerebral cortex.
Fekete CD, Goz RU, Dinallo S, Miralles CP, Chiou TT, Bear J, Fiondella CG, LoTurco JJ, De Blas AL
The Journal of comparative neurology (2017) 5255: 1291-1311. 147 021 IHC; tested species: rat
Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.
Li J, Han W, Pelkey KA, Duan J, Mao X, Wang YX, Craig MT, Dong L, Petralia RS, McBain CJ, Lu W, et al.
Neuron (2017) 964: 808-826.e8. 147 021 WB, ICC, IHC; tested species: mouse
Neuronal Dystroglycan Is Necessary for Formation and Maintenance of Functional CCK-Positive Basket Cell Terminals on Pyramidal Cells.
Früh S, Romanos J, Panzanelli P, Bürgisser D, Tyagarajan SK, Campbell KP, Santello M, Fritschy JM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3640: 10296-10313. 147 021 IHC; tested species: mouse
Early postnatal GABAA receptor modulation reverses deficits in neuronal maturation in a conditional neurodevelopmental mouse model of DISC1.
Saito A, Taniguchi Y, Rannals MD, Merfeld EB, Ballinger MD, Koga M, Ohtani Y, Gurley DA, Sedlak TW, Cross A, Moss SJ, et al.
Molecular psychiatry (2016) 2110: 1449-59. 147 021 IHC
Immunostaining for Homer reveals the majority of excitatory synapses in laminae I-III of the mouse spinal dorsal horn.
Gutierrez-Mecinas M, Kuehn ED, Abraira VE, Polgár E, Watanabe M, Todd AJ
Neuroscience (2016) 329: 171-81. 147 021 IHC
Forebrain-specific loss of synaptic GABAA receptors results in altered neuronal excitability and synaptic plasticity in mice.
O'Sullivan GA, Jedlicka P, Chen HX, Kalbouneh H, Ippolito A, Deller T, Nawrotzki RA, Kuhse J, Kalaidzidis YL, Kirsch J, Schwarzacher SW, et al.
Molecular and cellular neurosciences (2016) 72: 101-13. 147 021 IHC
Repetitive magnetic stimulation induces plasticity of inhibitory synapses.
Lenz M, Galanis C, Müller-Dahlhaus F, Opitz A, Wierenga CJ, Szabó G, Ziemann U, Deller T, Funke K, Vlachos A
Nature communications (2016) 7: 10020. 147 021 IHC
In vivo clonal overexpression of neuroligin 3 and neuroligin 2 in neurons of the rat cerebral cortex: Differential effects on GABAergic synapses and neuronal migration.
Fekete CD, Chiou TT, Miralles CP, Harris RS, Fiondella CG, Loturco JJ, De Blas AL
The Journal of comparative neurology (2015) 5239: 1359-78. 147 021 IHC
Activity-dependent inhibitory synapse remodeling through gephyrin phosphorylation.
Flores CE, Nikonenko I, Mendez P, Fritschy JM, Tyagarajan SK, Muller D
Proceedings of the National Academy of Sciences of the United States of America (2015) 1121: E65-72. 147 021 IHC
KCNQ5 K(+) channels control hippocampal synaptic inhibition and fast network oscillations.
Fidzinski P, Korotkova T, Heidenreich M, Maier N, Schuetze S, Kobler O, Zuschratter W, Schmitz D, Ponomarenko A, Jentsch TJ
Nature communications (2015) 6: 6254. 147 021 IHC
A protocol for concurrent high-quality immunohistochemical and biochemical analyses in adult mouse central nervous system.
Notter T, Panzanelli P, Pfister S, Mircsof D, Fritschy JM
The European journal of neuroscience (2014) 392: 165-75. 147 021 IHC, EM
Bassoon-disruption slows vesicle replenishment and induces homeostatic plasticity at a CNS synapse.
Mendoza Schulz A, Jing Z, Sánchez Caro JM, Wetzel F, Dresbach T, Strenzke N, Wichmann C, Moser T
The EMBO journal (2014) 335: 512-27. 147 021 IHC
Synaptic recruitment of gephyrin regulates surface GABAA receptor dynamics for the expression of inhibitory LTP.
Petrini EM, Ravasenga T, Hausrat TJ, Iurilli G, Olcese U, Racine V, Sibarita JB, Jacob TC, Moss SJ, Benfenati F, Medini P, et al.
Nature communications (2014) 5: 3921. 147 021 IHC; tested species: mouse
Reducing GABAA α5 receptor-mediated inhibition rescues functional and neuromorphological deficits in a mouse model of down syndrome.
Martínez-Cué C, Martínez P, Rueda N, Vidal R, García S, Vidal V, Corrales A, Montero JA, Pazos Á, Flórez J, Gasser R, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 339: 3953-66. 147 021 IHC; tested species: mouse
Brain inflammation induces post-synaptic changes during early synapse formation in adult-born hippocampal neurons.
Chugh D, Nilsson P, Afjei SA, Bakochi A, Ekdahl CT
Experimental neurology (2013) 250: 176-88. 147 021 IHC; tested species: mouse
A dramatic increase of C1q protein in the CNS during normal aging.
Stephan AH, Madison DV, Mateos JM, Fraser DA, Lovelett EA, Coutellier L, Kim L, Tsai HH, Huang EJ, Rowitch DH, Berns DS, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3333: 13460-74. 147 021 IHC; tested species: mouse
Amacrine and bipolar inputs to midget and parasol ganglion cells in marmoset retina.
Abbott CJ, Percival KA, Martin PR, Grünert U
Visual neuroscience (2012) 293: 157-68. 147 021 IHC; tested species: marmoset
Altered synaptic properties during integration of adult-born hippocampal neurons following a seizure insult.
Jackson J, Chugh D, Nilsson P, Wood J, Carlström K, Lindvall O, Ekdahl CT
PloS one (2012) 74: e35557. 147 021 IHC
Age-related changes of glycine receptor at the rat hippocampus: from the embryo to the adult.
Aroeira RI, Ribeiro JA, Sebastião AM, Valente CA
Journal of neurochemistry (2011) 1183: 339-53. 147 021 IHC; tested species: rat
Postsynaptic TrkC and presynaptic PTPσ function as a bidirectional excitatory synaptic organizing complex.
Takahashi H, Arstikaitis P, Prasad T, Bartlett TE, Wang YT, Murphy TH, Craig AM
Neuron (2011) 692: 287-303. 147 021 IHC; tested species: mouse,rat
Hyperexcitability, interneurons, and loss of GABAergic synapses in entorhinal cortex in a model of temporal lobe epilepsy.
Kumar SS, Buckmaster PS
The Journal of neuroscience : the official journal of the Society for Neuroscience (2006) 2617: 4613-23. 147 021 IHC; tested species: rat
Extrasynaptic homomeric glycine receptors in neurons of the rat trigeminal mesencephalic nucleus.
Bae JY, Lee JS, Ko SJ, Cho YS, Rah JC, Cho HJ, Park MJ, Bae YC
Brain structure & function (2018) : . 147 021 IHC, EM; tested species: rat
A protocol for concurrent high-quality immunohistochemical and biochemical analyses in adult mouse central nervous system.
Notter T, Panzanelli P, Pfister S, Mircsof D, Fritschy JM
The European journal of neuroscience (2014) 392: 165-75. 147 021 IHC, EM
Electron tomography on γ-aminobutyric acid-ergic synapses reveals a discontinuous postsynaptic network of filaments.
Linsalata AE, Chen X, Winters CA, Reese TS
The Journal of comparative neurology (2014) 5224: 921-36. 147 021 ICC, EM; tested species: rat
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com