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SY SY - Synaptic Systems SY SY - Synaptic Systems

Gephyrin - 147 018

A protein associated with inhibitory neurotransmitter receptors

Recombinant rabbit hybridoma supernatant
415.00 USD
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Cat. No. 147 018 200 µl hybridoma supernatant, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications WB: not recommended (see remarks)
IP: not recommended (see remarks)
ICC: 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-P/FFPE: not tested yet
Clone RbmAb7a
Subtype IgG1 (κ light chain)
Immunogen Native Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop N-terminus of gephyrin, surrounding phospho-serines 268 and 270.
Reactivity Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish.
Other species not tested yet.
Specificity Specific for the brain specific 93 kDa splice variant. (K.O. verified)
Remarks

WB: Clone 3B11 (cat. no. 147 111) highly recommended.

IP: Clone 3B11 (cat. no. 147 111) highly recommended.

IHC: For best results use the protocol of Schneider Gasser et al., 2006.
Alternatively AGR (10 mM citrate, pH 6.0, over night at 60°C) can be applied.

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents.
The antibody has been expressed in mammalian cells.

Datasheet 147_018.pdf


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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com