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SY SY - Synaptic Systems SY SY - Synaptic Systems

Gephyrin - 147 011

A protein associated with inhibitory neurotransmitter receptors

Monoclonal mouse purified IgG
415.00 USD
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Cat. No. 147 011 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: yes (see remarks)
IP: yes (see remarks)
ICC: 1 : 250 up to 1 : 500 image  
IHC: yes , frozen and paraffin sections (see remarks)
IHC-P/FFPE: not tested yet
EM: yes
Clone mAb7a
Subtype IgG1
Immunogen Recombinant protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop Epitop: AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reactivity Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish.
Other species not tested yet.
Specificity Specific for the brain specific 93 kDa splice variant; K.O. verified.
Remarks

WB: Clone 3B11 (cat. no. 147 111) highly recommended.

IP: Clone 3B11 (cat. no. 147 111) highly recommended.

IHC: Hybridoma supernatant (cat. no. 147 021) highly recommended. For best results use the protocol of Schneider Gasser et al., 2006.

Datasheet 147_011.pdf


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References for Gephyrin - 147 011

Extracellular signal-regulated kinase and glycogen synthase kinase 3β regulate gephyrin postsynaptic aggregation and GABAergic synaptic function in a calpain-dependent mechanism.
Tyagarajan SK, Ghosh H, Yévenes GE, Imanishi SY, Zeilhofer HU, Gerrits B, Fritschy JM
The Journal of biological chemistry (2013) 28814: 9634-47. 147 011 WB, IP
Extracellular signal-regulated kinase and glycogen synthase kinase 3β regulate gephyrin postsynaptic aggregation and GABAergic synaptic function in a calpain-dependent mechanism.
Tyagarajan SK, Ghosh H, Yévenes GE, Imanishi SY, Zeilhofer HU, Gerrits B, Fritschy JM
The Journal of biological chemistry (2013) 28814: 9634-47. 147 011 WB, IP
Depolarizing, inhibitory GABA type A receptor activity regulates GABAergic synapse plasticity via ERK and BDNF signaling.
Brady ML, Pilli J, Lorenz-Guertin JM, Das S, Moon CE, Graff N, Jacob TC
Neuropharmacology (2018) 128: 324-339. 147 011 ICC; tested species: rat
The antipsychotic drugs olanzapine and haloperidol modify network connectivity and spontaneous activity of neural networks in vitro.
Dzyubenko E, Juckel G, Faissner A
Scientific reports (2017) 71: 11609. 147 011 ICC; tested species: mouse
Alpha subunit-dependent glycine receptor clustering and regulation of synaptic receptor numbers.
Patrizio A, Renner M, Pizzarelli R, Triller A, Specht CG
Scientific reports (2017) 71: 10899. 147 011 ICC; tested species: rat
Nanoscale Molecular Reorganization of the Inhibitory Postsynaptic Density Is a Determinant of GABAergic Synaptic Potentiation.
Pennacchietti F, Vascon S, Nieus T, Rosillo C, Das S, Tyagarajan SK, Diaspro A, Del Bue A, Petrini EM, Barberis A, Cella Zanacchi F, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2017) 377: 1747-1756. 147 011 ICC
Adaptive-illumination STED nanoscopy.
Heine J, Reuss M, Harke B, D'Este E, Sahl SJ, Hell SW
Proceedings of the National Academy of Sciences of the United States of America (2017) 11437: 9797-9802. 147 011 ICC; tested species: rat
Radixin regulates synaptic GABAA receptor density and is essential for reversal learning and short-term memory.
Hausrat TJ, Muhia M, Gerrow K, Thomas P, Hirdes W, Tsukita S, Heisler FF, Herich L, Dubroqua S, Breiden P, Feldon J, et al.
Nature communications (2015) 6: 6872. 147 011 ICC
A negative feedback loop controls NMDA receptor function in cortical interneurons via neuregulin 2/ErbB4 signalling.
Vullhorst D, Mitchell RM, Keating C, Roychowdhury S, Karavanova I, Tao-Cheng JH, Buonanno A
Nature communications (2015) 6: 7222. 147 011 ICC
Lipid binding defects and perturbed synaptogenic activity of a Collybistin R290H mutant that causes epilepsy and intellectual disability.
Papadopoulos T, Schemm R, Grubmüller H, Brose N
The Journal of biological chemistry (2015) 29013: 8256-70. 147 011 ICC
Activity-regulated trafficking of the palmitoyl-acyl transferase DHHC5.
Brigidi GS, Santyr B, Shimell J, Jovellar B, Bamji SX
Nature communications (2015) 6: 8200. 147 011 ICC
Plexin-B3 suppresses excitatory and promotes inhibitory synapse formation in rat hippocampal neurons.
Laht P, Tammaru E, Otsus M, Rohtla J, Tiismus L, Veske A
Experimental cell research (2015) 3352: 269-78. 147 011 ICC
Synaptic abnormalities and cytoplasmic glutamate receptor aggregates in contactin associated protein-like 2/Caspr2 knockout neurons.
Varea O, Martin-de-Saavedra MD, Kopeikina KJ, Schürmann B, Fleming HJ, Fawcett-Patel JM, Bach A, Jang S, Peles E, Kim E, Penzes P, et al.
Proceedings of the National Academy of Sciences of the United States of America (2015) 11219: 6176-81. 147 011 ICC
Synaptic recruitment of gephyrin regulates surface GABAA receptor dynamics for the expression of inhibitory LTP.
Petrini EM, Ravasenga T, Hausrat TJ, Iurilli G, Olcese U, Racine V, Sibarita JB, Jacob TC, Moss SJ, Benfenati F, Medini P, et al.
Nature communications (2014) 5: 3921. 147 011 ICC; tested species: mouse
Dlg5 regulates dendritic spine formation and synaptogenesis by controlling subcellular N-cadherin localization.
Wang SH, Celic I, Choi SY, Riccomagno M, Wang Q, Sun LO, Mitchell SP, Vasioukhin V, Huganir RL, Kolodkin AL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3438: 12745-61. 147 011 ICC; tested species: mouse
Genetic evidence for the adhesion protein IgSF9/Dasm1 to regulate inhibitory synapse development independent of its intracellular domain.
Mishra A, Traut MH, Becker L, Klopstock T, Stein V, Klein R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3412: 4187-99. 147 011 ICC, IHC; tested species: mouse
High content image analysis identifies novel regulators of synaptogenesis in a high-throughput RNAi screen of primary neurons.
Nieland TJ, Logan DJ, Saulnier J, Lam D, Johnson C, Root DE, Carpenter AE, Sabatini BL
PloS one (2014) 93: e91744. 147 011 ICC; tested species: mouse
Proteomic analysis of glycine receptor β subunit (GlyRβ)-interacting proteins: evidence for syndapin I regulating synaptic glycine receptors.
Del Pino I, Koch D, Schemm R, Qualmann B, Betz H, Paarmann I
The Journal of biological chemistry (2014) 28916: 11396-409. 147 011 ICC; tested species: rat
Quantitative nanoscopy of inhibitory synapses: counting gephyrin molecules and receptor binding sites.
Specht CG, Izeddin I, Rodriguez PC, El Beheiry M, Rostaing P, Darzacq X, Dahan M, Triller A
Neuron (2013) 792: 308-21. 147 011 ICC; tested species: mouse
Differential control of thrombospondin over synaptic glycine and AMPA receptors in spinal cord neurons.
Hennekinne L, Colasse S, Triller A, Renner M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3328: 11432-9. 147 011 ICC; tested species: rat
TNF-α downregulates inhibitory neurotransmission through protein phosphatase 1-dependent trafficking of GABA(A) receptors.
Pribiag H, Stellwagen D
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3340: 15879-93. 147 011 ICC; tested species: rat
Tracing synaptic connectivity onto embryonic stem cell-derived neurons.
Garcia I, Huang L, Ung K, Arenkiel BR
Stem cells (Dayton, Ohio) (2012) 3010: 2140-51. 147 011 ICC; tested species: mouse
Effects of distinct collybistin isoforms on the formation of GABAergic synapses in hippocampal neurons.
Körber C, Richter A, Kaiser M, Schlicksupp A, Mükusch S, Kuner T, Kirsch J, Kuhse J
Molecular and cellular neurosciences (2012) 503-4: 250-9. 147 011 ICC
Molecular basis of the γ-aminobutyric acid A receptor α3 subunit interaction with the clustering protein gephyrin.
Tretter V, Kerschner B, Milenkovic I, Ramsden SL, Ramerstorfer J, Saiepour L, Maric HM, Moss SJ, Schindelin H, Harvey RJ, Sieghart W, et al.
The Journal of biological chemistry (2011) 28643: 37702-11. 147 011 ICC; tested species: mouse
Irregular RNA splicing curtails postsynaptic gephyrin in the cornu ammonis of patients with epilepsy.
Förstera B, Belaidi AA, Jüttner R, Bernert C, Tsokos M, Lehmann TN, Horn P, Dehnicke C, Schwarz G, Meier JC
Brain : a journal of neurology (2010) 133Pt 12: 3778-94. 147 011 ICC
Astrocyte secreted proteins selectively increase hippocampal GABAergic axon length, branching, and synaptogenesis.
Hughes EG, Elmariah SB, Balice-Gordon RJ
Molecular and cellular neurosciences (2010) 431: 136-45. 147 011 ICC; tested species: rat
Induction of GABAergic postsynaptic differentiation by alpha-neurexins.
Kang Y, Zhang X, Dobie F, Wu H, Craig AM
The Journal of biological chemistry (2008) 2834: 2323-34. 147 011 ICC; tested species: rat
Activity-dependent regulation of inhibitory synaptic transmission in hippocampal neurons.
Hartman KN, Pal SK, Burrone J, Murthy VN
Nature neuroscience (2006) 95: 642-9. 147 011 ICC
Neuronal cotransport of glycine receptor and the scaffold protein gephyrin.
Maas C, Tagnaouti N, Loebrich S, Behrend B, Lappe-Siefke C, Kneussel M
The Journal of cell biology (2006) 1723: 441-51. 147 011 ICC
Structure function and splice site analysis of the synaptogenic activity of the neurexin-1 beta LNS domain.
Graf ER, Kang Y, Hauner AM, Craig AM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2006) 2616: 4256-65. 147 011 ICC
Gephyrin regulates the cell surface dynamics of synaptic GABAA receptors.
Jacob TC, Bogdanov YD, Magnus C, Saliba RS, Kittler JT, Haydon PG, Moss SJ
The Journal of neuroscience : the official journal of the Society for Neuroscience (2005) 2545: 10469-78. 147 011 ICC
A gephyrin-related mechanism restraining glycine receptor anchoring at GABAergic synapses.
Meier J, Grantyn R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2004) 246: 1398-405. 147 011 ICC
GSK3 and KIF5 regulate activity-dependent sorting of gephyrin between axons and dendrites.
Rathgeber L, Gromova KV, Schaefer I, Breiden P, Lohr C, Kneussel M
European journal of cell biology () 943-4: 173-8. 147 011 ICC
The excitatory/inhibitory input to orexin/hypocretin neuron soma undergoes day/night reorganization.
Laperchia C, Imperatore R, Azeez IA, Del Gallo F, Bertini G, Grassi-Zucconi G, Cristino L, Bentivoglio M
Brain structure & function (2017) 2228: 3847-3859. 147 011 IHC; tested species: mouse
Differential role of GABAA receptors and neuroligin 2 for perisomatic GABAergic synapse formation in the hippocampus.
Panzanelli P, Früh S, Fritschy JM
Brain structure & function (2017) 2229: 4149-4161. 147 011 IHC; tested species: mouse
Iterative expansion microscopy.
Chang JB, Chen F, Yoon YG, Jung EE, Babcock H, Kang JS, Asano S, Suk HJ, Pak N, Tillberg PW, Wassie AT, et al.
Nature methods (2017) 146: 593-599. 147 011 IHC; tested species: mouse
Disinhibition of somatostatin-positive GABAergic interneurons results in an anxiolytic and antidepressant-like brain state.
Fuchs T, Jefferson SJ, Hooper A, Yee PH, Maguire J, Luscher B
Molecular psychiatry (2017) 226: 920-930. 147 011 IHC
Enrichment of GABAA Receptor α-Subunits on the Axonal Initial Segment Shows Regional Differences.
Gao Y, Heldt SA
Frontiers in cellular neuroscience (2016) 10: 39. 147 011 IHC
Postsynaptic gephyrin clustering controls the development of adult-born granule cells in the olfactory bulb.
Deprez F, Pallotto M, Vogt F, Grabiec M, Virtanen MA, Tyagarajan SK, Panzanelli P, Fritschy JM
The Journal of comparative neurology (2015) 52313: 1998-2016. 147 011 IHC, EM
Dendrodendritic synapses in the mouse olfactory bulb external plexiform layer.
Bartel DL, Rela L, Hsieh L, Greer CA
The Journal of comparative neurology (2015) 5238: 1145-61. 147 011 IHC
Interneuron- and GABA(A) receptor-specific inhibitory synaptic plasticity in cerebellar Purkinje cells.
He Q, Duguid I, Clark B, Panzanelli P, Patel B, Thomas P, Fritschy JM, Smart TG
Nature communications (2015) 6: 7364. 147 011 IHC
A unique ion channel clustering domain on the axon initial segment of mammalian neurons.
King AN, Manning CF, Trimmer JS
The Journal of comparative neurology (2014) 52211: 2594-608. 147 011 IHC; tested species: rat
Genetic evidence for the adhesion protein IgSF9/Dasm1 to regulate inhibitory synapse development independent of its intracellular domain.
Mishra A, Traut MH, Becker L, Klopstock T, Stein V, Klein R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3412: 4187-99. 147 011 ICC, IHC; tested species: mouse
Gephyrin clusters are absent from small diameter primary afferent terminals despite the presence of GABA(A) receptors.
Lorenzo LE, Godin AG, Wang F, St-Louis M, Carbonetto S, Wiseman PW, Ribeiro-da-Silva A, De Koninck Y
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 3424: 8300-17. 147 011 IHC; tested species: rat
Spatial relationships between GABAergic and glutamatergic synapses on the dendrites of distinct types of mouse retinal ganglion cells across development.
Bleckert A, Parker ED, Kang Y, Pancaroglu R, Soto F, Lewis R, Craig AM, Wong RO
PloS one (2013) 87: e69612. 147 011 IHC; tested species: mouse
Distribution of gephyrin-immunoreactivity in the trigeminal motor nucleus: an immunohistochemical study in rats.
Li Z, Ge S, Zhang F, Zhang T, Mizuno N, Hioki H, Kaneko T, Gao G, Li J
Anatomical record (Hoboken, N.J. : 2007) (2012) 2954: 641-51. 147 011 IHC, EM; tested species: rat
Somatostatin varicosities contain the vesicular GABA transporter and contact orexin neurons in the hypothalamus.
Toossi H, Del Cid-Pellitero E, Stroh T, Jones BE
The European journal of neuroscience (2012) 3610: 3388-95. 147 011 IHC
Differential distribution of glycine receptor subtypes at the rat calyx of Held synapse.
Hruskova B, Trojanova J, Kulik A, Kralikova M, Pysanenko K, Bures Z, Syka J, Trussell LO, Turecek R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3247: 17012-24. 147 011 IHC, EM
Distinct mechanisms regulate GABAA receptor and gephyrin clustering at perisomatic and axo-axonic synapses on CA1 pyramidal cells.
Panzanelli P, Gunn BG, Schlatter MC, Benke D, Tyagarajan SK, Scheiffele P, Belelli D, Lambert JJ, Rudolph U, Fritschy JM
The Journal of physiology (2011) 589Pt 20: 4959-80. 147 011 IHC, EM; tested species: mouse
Quantitative study of NPY-expressing GABAergic neurons and axons in rat spinal dorsal horn.
Polgár E, Sardella TC, Watanabe M, Todd AJ
The Journal of comparative neurology (2011) 5196: 1007-23. 147 011 IHC; tested species: rat
Mixed GABA-glycine synapses delineate a specific topography in the nucleus tractus solitarii of adult rat.
Dufour A, Tell F, Kessler JP, Baude A
The Journal of physiology (2010) 588Pt 7: 1097-115. 147 011 IHC; tested species: rat
Excitatory-inhibitory relationship in the fascia dentata in the Ts65Dn mouse model of Down syndrome.
Belichenko PV, Kleschevnikov AM, Masliah E, Wu C, Takimoto-Kimura R, Salehi A, Mobley WC
The Journal of comparative neurology (2009) 5124: 453-66. 147 011 IHC; tested species: mouse
Developmental maturation of synaptic and extrasynaptic GABAA receptors in mouse thalamic ventrobasal neurones.
Peden DR, Petitjean CM, Herd MB, Durakoglugil MS, Rosahl TW, Wafford K, Homanics GE, Belelli D, Fritschy JM, Lambert JJ
The Journal of physiology (2008) 5864: 965-87. 147 011 IHC; tested species: mouse
Compensatory alteration of inhibitory synaptic circuits in cerebellum and thalamus of gamma-aminobutyric acid type A receptor alpha1 subunit knockout mice.
Kralic JE, Sidler C, Parpan F, Homanics GE, Morrow AL, Fritschy JM
The Journal of comparative neurology (2006) 4954: 408-21. 147 011 IHC
Synaptic destabilization by neuronal Nogo-A.
Aloy EM, Weinmann O, Pot C, Kasper H, Dodd DA, Rülicke T, Rossi F, Schwab ME
Brain cell biology (2006) 352-3: 137-56. 147 011 IHC
Cerebellar synaptic defects and abnormal motor behavior in mice lacking alpha- and beta-dystrobrevin.
Grady RM, Wozniak DF, Ohlemiller KK, Sanes JR
The Journal of neuroscience : the official journal of the Society for Neuroscience (2006) 2611: 2841-51. 147 011 IHC
Immunofluorescence in brain sections: simultaneous detection of presynaptic and postsynaptic proteins in identified neurons.
Schneider Gasser EM, Straub CJ, Panzanelli P, Weinmann O, Sassoè-Pognetto M, Fritschy JM
Nature protocols (2006) 14: 1887-97. 147 011 IHC
Neurochemical anatomy of the zebrafish retina as determined by immunocytochemistry.
Yazulla S, Studholme KM
Journal of neurocytology (2001) 307: 551-92. 147 011 IHC; tested species: zebrafish
Glycinergic miniature synaptic currents and receptor cluster sizes differ between spinal cord interneurons.
Oleskevich S, Alvarez FJ, Walmsley B
Journal of neurophysiology (1999) 821: 312-9. 147 011 IHC
Dual requirement for gephyrin in glycine receptor clustering and molybdoenzyme activity.
Feng G, Tintrup H, Kirsch J, Nichol MC, Kuhse J, Betz H, Sanes JR
Science (New York, N.Y.) (1998) 2825392: 1321-4. 147 011 IHC; KO verified; tested species: mouse
Postsynaptic gephyrin clustering controls the development of adult-born granule cells in the olfactory bulb.
Deprez F, Pallotto M, Vogt F, Grabiec M, Virtanen MA, Tyagarajan SK, Panzanelli P, Fritschy JM
The Journal of comparative neurology (2015) 52313: 1998-2016. 147 011 IHC, EM
Distribution of gephyrin-immunoreactivity in the trigeminal motor nucleus: an immunohistochemical study in rats.
Li Z, Ge S, Zhang F, Zhang T, Mizuno N, Hioki H, Kaneko T, Gao G, Li J
Anatomical record (Hoboken, N.J. : 2007) (2012) 2954: 641-51. 147 011 IHC, EM; tested species: rat
Differential distribution of glycine receptor subtypes at the rat calyx of Held synapse.
Hruskova B, Trojanova J, Kulik A, Kralikova M, Pysanenko K, Bures Z, Syka J, Trussell LO, Turecek R
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3247: 17012-24. 147 011 IHC, EM
Synaptic reorganization in the adult rat's ventral cochlear nucleus following its total sensory deafferentation.
Hildebrandt H, Hoffmann NA, Illing RB
PloS one (2011) 68: e23686. 147 011 EM; tested species: rat
Distinct mechanisms regulate GABAA receptor and gephyrin clustering at perisomatic and axo-axonic synapses on CA1 pyramidal cells.
Panzanelli P, Gunn BG, Schlatter MC, Benke D, Tyagarajan SK, Scheiffele P, Belelli D, Lambert JJ, Rudolph U, Fritschy JM
The Journal of physiology (2011) 589Pt 20: 4959-80. 147 011 IHC, EM; tested species: mouse
Monoclonal antibodies and peptide mapping reveal structural similarities between the subunits of the glycine receptor of rat spinal cord.
Pfeiffer F, Simler R, Grenningloh G, Betz H
Proceedings of the National Academy of Sciences of the United States of America (1984) 8122: 7224-7. 147 011
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com