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SY SY - Synaptic Systems SY SY - Synaptic Systems

Gephyrin - 147 008

A protein associated with inhibitory neurotransmitter receptors

Recombinant rabbit purified IgG
415.00 USD
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Cat. No. 147 008 50 µg purified IgG, lyophilized. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: yes (see remarks)
IP: yes (see remarks)
ICC: 1 : 500 gallery  
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-P/FFPE: not tested yet
Clone RbmAb7a
Subtype IgG1 (κ light chain)
Immunogen Native Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)
Epitop Epitop: AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reactivity Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish.
Other species not tested yet.
Specificity Specific for the brain specific 93 kDa splice variant. (K.O. verified)
Remarks

WB: Clone 3B11 (cat. no. 147 111) highly recommended.

IP: Clone 3B11 (cat. no. 147 111) highly recommended.

IHC: For best results use the protocol of Schneider Gasser et al., 2006.
Alternatively AGR (10 mM citrate, pH 6.0, over night at 60°C) can be applied.

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents.
The antibody has been expressed in mammalian cells.

Datasheet 147_008.pdf


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References for Gephyrin - 147 008

Identification of a Core Amino Acid Motif within the α Subunit of GABAARs that Promotes Inhibitory Synaptogenesis and Resilience to Seizures.
Nathanson AJ, Zhang Y, Smalley JL, Ollerhead TA, Rodriguez Santos MA, Andrews PM, Wobst HJ, Moore YE, Brandon NJ, Hines RM, Davies PA, et al.
Cell reports (2019) 283: 670-681.e8. 147 008 ICC; tested species: mouse
NPAS4 recruits CCK basket cell synapses and enhances cannabinoid-sensitive inhibition in the mouse hippocampus.
Hartzell AL, Martyniuk KM, Brigidi GS, Heinz DA, Djaja NA, Payne A, Bloodgood BL
eLife (2018) 7: . 147 008 IHC; tested species: mouse
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com