Gephyrin - 147 008
A protein associated with inhibitory neurotransmitter receptors
Recombinant rabbit purified IgG
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|Cat. No. 147 008||50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: yes (see remarks)
IP: yes (see remarks)
ICC: 1 : 500 gallery
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery
IHC-P/FFPE: not tested yet
|Subtype||IgG1 (κ light chain)|
|Immunogen||Native Protein corresponding to AA 1 to 768 from rat Gephyrin (UniProt Id: Q03555)|
Epitop: AA 264 to 276 from rat Gephyrin (UniProt Id: Q03555)
Reacts with: human (Q9NQX3), rat (Q03555), mouse (Q8BUV3), pig, goldfish, zebrafish.
Other species not tested yet.
|Specificity||Specific for the brain specific 93 kDa splice variant. (K.O. verified)|
This antibody is a chimeric antibody based on the well known monoclonal mouse antibody mAb7a. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents.
WB: Clone 3B11 (cat. no. 147 111) is highly recommended.
IP: Clone 3B11 (cat. no. 147 111) is highly recommended.
IHC: For best results use the protocol of Schneider Gasser et al., 2006.