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GABA transporter3 antibody - 274 304

GATs are neurotransmitter transporters involved in the termination of GABA transmission
Guinea pig polyclonal antiserum
Cat. No.: 274 304
Amount: 100 µl
Price: $365.00
Cat. No. 274 304 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
ExM: yes
Immunogen Synthetic peptide corresponding to AA 612 to 627 from mouse GABA transporter3 (UniProt Id: P31650)
Reactivity Reacts with: rat (P31647), mouse (P31650).
Other species not tested yet.
Matching control protein/peptide 274-3P
Remarks

WB:  GABA transporter 3 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 274_304.pdf

References for GABA transporter3 - 274 304

Extracellular Vesicles from Hyperammonemic Rats Induce Neuroinflammation and Motor Incoordination in Control Rats.
Izquierdo-Altarejos P, Cabrera-Pastor A, Gonzalez-King H, Montoliu C, Felipo V
Cells (2020) 93: . 274 304 WB; tested species: rat
Neurons Induce Tiled Astrocytes with Branches That Avoid Each Other.
Hayashi MK, Sato K, Sekino Y
International journal of molecular sciences (2022) 238: . 274 304 ICC; tested species: rat
Light microscopic and heterogeneity analysis of astrocytes in the common marmoset brain.
Muñoz Y, Cuevas-Pacheco F, Quesseveur G, Murai KK
Journal of neuroscience research (2021) : . 274 304 IHC; tested species: marmoset
Doublecortin-like expressing astrocytes of the suprachiasmatic nucleus are implicated in the biosynthesis of vasopressin and influences circadian rhythms.
Coomans C, Saaltink DJ, Deboer T, Tersteeg M, Lanooij S, Schneider AF, Mulder A, van Minnen J, Jost C, Koster AJ, Vreugdenhil E, et al.
Glia (2021) 6911: 2752-2766. 274 304 IHC; tested species: mouse
Astrocytes detect and upregulate transmission at inhibitory synapses of somatostatin interneurons onto pyramidal cells.
Matos M, Bosson A, Riebe I, Reynell C, Vallée J, Laplante I, Panatier A, Robitaille R, Lacaille JC
Nature communications (2018) 91: 4254. 274 304 IHC; tested species: mouse
Olig2-Lineage Astrocytes: A Distinct Subtype of Astrocytes That Differs from GFAP Astrocytes.
Tatsumi K, Isonishi A, Yamasaki M, Kawabe Y, Morita-Takemura S, Nakahara K, Terada Y, Shinjo T, Okuda H, Tanaka T, Wanaka A, et al.
Frontiers in neuroanatomy (2018) 12: 8. 274 304 IHC; tested species: mouse
Structural Heterogeneity of the GABAergic Tripartite Synapse.
Brunskine C, Passlick S, Henneberger C
Cells (2022) 1119: . 274 304 ExM; tested species: mouse
Cat. No.: 274 304
Amount: 100 µl
Price: $365.00
Extracellular Vesicles from Hyperammonemic Rats Induce Neuroinflammation and Motor Incoordination in Control Rats.
Izquierdo-Altarejos P, Cabrera-Pastor A, Gonzalez-King H, Montoliu C, Felipo V
Cells (2020) 93: . 274 304 WB; tested species: rat
Neurons Induce Tiled Astrocytes with Branches That Avoid Each Other.
Hayashi MK, Sato K, Sekino Y
International journal of molecular sciences (2022) 238: . 274 304 ICC; tested species: rat
Light microscopic and heterogeneity analysis of astrocytes in the common marmoset brain.
Muñoz Y, Cuevas-Pacheco F, Quesseveur G, Murai KK
Journal of neuroscience research (2021) : . 274 304 IHC; tested species: marmoset
Doublecortin-like expressing astrocytes of the suprachiasmatic nucleus are implicated in the biosynthesis of vasopressin and influences circadian rhythms.
Coomans C, Saaltink DJ, Deboer T, Tersteeg M, Lanooij S, Schneider AF, Mulder A, van Minnen J, Jost C, Koster AJ, Vreugdenhil E, et al.
Glia (2021) 6911: 2752-2766. 274 304 IHC; tested species: mouse
Astrocytes detect and upregulate transmission at inhibitory synapses of somatostatin interneurons onto pyramidal cells.
Matos M, Bosson A, Riebe I, Reynell C, Vallée J, Laplante I, Panatier A, Robitaille R, Lacaille JC
Nature communications (2018) 91: 4254. 274 304 IHC; tested species: mouse
Olig2-Lineage Astrocytes: A Distinct Subtype of Astrocytes That Differs from GFAP Astrocytes.
Tatsumi K, Isonishi A, Yamasaki M, Kawabe Y, Morita-Takemura S, Nakahara K, Terada Y, Shinjo T, Okuda H, Tanaka T, Wanaka A, et al.
Frontiers in neuroanatomy (2018) 12: 8. 274 304 IHC; tested species: mouse
Structural Heterogeneity of the GABAergic Tripartite Synapse.
Brunskine C, Passlick S, Henneberger C
Cells (2022) 1119: . 274 304 ExM; tested species: mouse
Background
γ-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. After the release of GABA from synaptic vesicles into the synaptic cleft during neurotransmission, GABA transporters (GATs) remove extracellular GABA by reuptake into the presynaptic terminal.
Three GABA transporters are described so far of which only GAT 1 and GAT 3 are expressed in the brain.