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SY SY - Synaptic Systems SY SY - Synaptic Systems

GABA-A receptor β3 - 224 411

Receptors mediating inhibitory neurotransmission

Monoclonal mouse purified IgG
415.00 USD
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Cat. No. 224 411 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 500 up to 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC-P/FFPE: not tested yet
Clone 261F6
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 344 to 429 from mouse GABA-A receptor β3 (UniProt Id: P63080)
Epitop Epitop: AA 344 to 429 from mouse GABA-A receptor β3 (UniProt Id: P63080)
Reactivity Reacts with: rat (P63079), mouse (P63080).
Other species not tested yet.
Specificity Specific for GABA-A receptor β3.
Remarks

WB: Less sensitive compared to the polyclonal antibodies.

IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).
recommended protocol

Datasheet 224_411.pdf


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References for GABA-A receptor beta3 - 224 411

The Autism-Related Protein PX-RICS Mediates GABAergic Synaptic Plasticity in Hippocampal Neurons and Emotional Learning in Mice.
Nakamura T, Sakaue F, Nasu-Nishimura Y, Takeda Y, Matsuura K, Akiyama T
EBioMedicine (2018) : . 224 411 WB; tested species: mouse
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com