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GABA-A receptor α1 - 224 211

Receptors mediating inhibitory neurotransmission

Monoclonal mouse purified IgM
415.00 USD
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Cat. No. 224 211 100 µg purified IgM, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 up to 1 : 2000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 (see remarks) gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: not tested yet
EM: yes
Clone 53D3
Subtype IgM (κ light chain)
Immunogen Synthetic peptide corresponding to AA 28 to 43 from rat GABA-A receptor α1 (UniProt Id: P62813)
Epitop Epitop: AA 28 to 43 from rat GABA-A receptor α1 (UniProt Id: P62813)
Reactivity Reacts with: rat (P62813), mouse (P62812).
Other species not tested yet.
Specificity Specific for GABA-A receptor α1.
matching control protein/peptide 224-2P
Remarks

ICC: This antibody is PFA fixation sensitive, use only mild fixation (2 % PFA).Best results are obtained by application of antibodies on living cells. After washingcells with bound antibodies, they can be fixed and visualized with secondaryreagents.

IHC: This antibody requires mild fixation. recommended protocol

Datasheet 224_211.pdf


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References for GABA-A receptor alpha1 - 224 211

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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com