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DARPP32 peptide - 382-0P

DARPP32 is a dopamine and cAMP regulated phosphoprotein
control peptide
Cat. No.: 382-0P
Amount: 100 µg
Price: $105.00
Cat. No. 382-0P 100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Control peptides should be stored at -20°C when still lyophilized!
Applications
 
Immunogen Synthetic peptide corresponding to AA 148 to 166 from mouse Darpp32 (UniProt Id: Q60829)
Recommended dilution Optimal concentrations should be determined by the end-user.
Matching antibodies 382 002, 382 003, 382 004, 382 005
Remarks

This control peptide consists of the synthetic peptide (aa 148-166 of mouse DARPP 32) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Data sheet 382-0p.pdf
Cat. No.: 382-0P
Amount: 100 µg
Price: $105.00
Background
The dopamine and cAMP regulated phosphoprotein 32 kDa (DARPP 32), also known as PPP1R1B, is phosphorylated in a dopamine dependent manner. Stimulation of the dopamine receptor DRD 1 increases cAMP levels resulting in DARPP 32 phosphorylation. It is a commonly used marker for striatal medium spiny neurons (MSNs).
Protocols