Synaptic Systems - Cre-Recombinase

Cat. No.: 257 003

Amount: 50 µg

Price: $385.00

Cat. No. 257 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: external data (see remarks) IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 2000 up to 1 : 5000 gallery
Immunogen	Full length recombinant Cre-recombinase from Bacteriophage P1. (UniProt Id: P06956)
Specificity	Recognizes Cre- and iCre-recombinase.
Remarks	WB: This antibody has been successfully used and published for this application by customers. It has not been validated using our standard protocol (see “application” references).
Data sheet	257_003.pdf

References for Cre-Recombinase - 257 003

WB (1)
IHC (12)

CYFIP2 p.Arg87Cys Causes Neurological Defects and Degradation of CYFIP2.
Kang M, Zhang Y, Kang HR, Kim S, Ma R, Yi Y, Lee S, Kim Y, Li H, Jin C, Lee D, et al.
Annals of neurology (2023) 931: 155-163. 257 003 WB; tested species: mouse

GluA1-containing AMPA receptors are necessary for sparse memory engram formation.
Willems TS, Xiong H, Kessels HW, Lesuis SL
Neurobiology of learning and memory (2025) 218: 108031. 257 003 IHC; tested species: mouse

A divergent astrocytic response to stress alters activity patterns via distinct mechanisms in male and female mice.
Depaauw-Holt LR, Duquenne M, Hamane S, Peyrard S, Rogers B, Ireland C, Nasu Y, Fulton S, Bosson A, Alquier T, Murphy-Royal C, et al.
Nature communications (2025) 161: 6372. 257 003 IHC; tested species: mouse

Cell-type-specific representation of spatial context in the rat prefrontal cortex.
Brünner H, Kim H, Ährlund-Richter S, van Lunteren JA, Crestani AP, Meletis K, Carlén M
iScience (2024) 275: 109743. 257 003 IHC; tested species: rat

Context-dependent requirement of G protein coupling for Latrophilin-2 in target selection of hippocampal axons.
Pederick DT, Perry-Hauser NA, Meng H, He Z, Javitch JA, Luo L
eLife (2023) 12: . 257 003 IHC; tested species: mouse

Human OPRM1 and murine Oprm1 promoter driven viral constructs for genetic access to μ-opioidergic cell types.
Salimando GJ, Tremblay S, Kimmey BA, Li J, Rogers SA, Wojick JA, McCall NM, Wooldridge LM, Rodrigues A, Borner T, Gardiner KL, et al.
Nature communications (2023) 141: 5632. 257 003 IHC; tested species: mouse

A critical period of translational control during brain development at codon resolution.
Harnett D, Ambrozkiewicz MC, Zinnall U, Rusanova A, Borisova E, Drescher AN, Couce-Iglesias M, Villamil G, Dannenberg R, Imami K, Münster-Wandowski A, et al.
Nature structural & molecular biology (2022) 2912: 1277-1290. 257 003 IHC; tested species: mouse

The role of α-tubulin tyrosination in controlling the structure and function of hippocampal neurons.
Hosseini S, van Ham M, Erck C, Korte M, Michaelsen-Preusse K
Frontiers in molecular neuroscience (2022) 15: 931859. 257 003 IHC; tested species: mouse

Cerebellin-2 regulates a serotonergic dorsal raphe circuit that controls compulsive behaviors.
Seigneur E, Wang J, Dai J, Polepalli J, Südhof TC
Molecular psychiatry (2021) : . 257 003 IHC; tested species: mouse

Npas1+-Nkx2.1+ Neurons Are an Integral Part of the Cortico-pallido-cortical Loop.
Abecassis ZA, Berceau BL, Win PH, García D, Xenias HS, Cui Q, Pamukcu A, Cherian S, Hernández VM, Chon U, Lim BK, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) 404: 743-768. 257 003 IHC; tested species: mouse

Regulation of BDNF Release by ARMS/Kidins220 through Modulation of Synaptotagmin-IV Levels.
López-Benito S, Sánchez-Sánchez J, Brito V, Calvo L, Lisa S, Torres-Valle M, Palko ME, Vicente-García C, Fernández-Fernández S, Bolaños JP, Ginés S, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2018) 3823: 5415-5428. 257 003 IHC; tested species: mouse

Polarity Acquisition in Cortical Neurons Is Driven by Synergistic Action of Sox9-Regulated Wwp1 and Wwp2 E3 Ubiquitin Ligases and Intronic miR-140.
Ambrozkiewicz MC, Schwark M, Kishimoto-Suga M, Borisova E, Hori K, Salazar-Lázaro A, Rusanova A, Altas B, Piepkorn L, Bessa P, Schaub T, et al.
Neuron (2018) : . 257 003 IHC; tested species: mouse

Satb2Cre/+ mouse as a tool to investigate cell fate determination in the developing neocortex.
Ambrozkiewicz MC, Bessa P, Salazar-Lázaro A, Salina V, Tarabykin V
Journal of neuroscience methods (2017) 291: 113-121. 257 003 IHC; tested species: mouse

Cat. No.: 257 003

Amount: 50 µg

Price: $385.00

References
WB (1)
IHC (12)

ICC

IHC

IHC-P

Background

Cre-Recombinase is a 38 kDa type 1 topoisomerase from bacteriophage P1. It is highly specific for a 34 bp DNA sequence (loxP) found in P1 DNA and catalyzes site-specific recombination between two 34-base-pair LOXP recognition sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. Its highly specific catalytic activity makes it a valuable tool for the generation of conditional or tissue specific mutants.

1 ) Ferguson BW et al., PLoS ONE (2012)
2 ) Lanza AM et al., Biotechnol J (2012)
3 ) Smedley D et al., Methods (2011)
4 ) Brault V et al., Handb Exp Pharmacol (2007)
5 ) Abremski K et al., J. Biol. Chem. (1984)
6 ) Hoess R et al., Cold Spring Harb. Symp. Quant. Biol. (1984)

Protocols

ICC

PFA fixation
Pre-adsorption protocol

IHC

Free floating
Slide mounted
Tissue preparation
Pre-adsorption protocol

IHC-P

Chromogenic detection
Fluorescent detection
Tissue preparation
Pre-adsorption protocol

Other products for this target

Cre-Recombinase antibody - 257 003

References for Cre-Recombinase - 257 003