Collybistin antibody - 261 111

Collybistin is a protein involved in gephyrin clustering

Mouse monoclonal purified IgG

Cat. No.: 261 111

Amount: 100 µg

Price: $420.00

Cat. No. 261 111	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: yes ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not recommended IHC-Fr: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	197A10
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 471 from rat Collybistin (UniProt Id: Q9QX73)
Epitop	AA 4 to 229 from rat Collybistin (UniProt Id: Q9QX73)
Reactivity	Reacts with: rat (Q9QX73), mouse (Q3UTH8). Other species not tested yet.
Specificity	Specific for collybistin, immunogen present in all three described splice-variants.
Remarks	IHC-Fr: Acetone fixation is recommended.
Data sheet	261_111.pdf

Cat. No.: 261 111

Amount: 100 µg

Price: $420.00

Collybistin (red) and Gephyrin (green) staining in a fresh-frozen mouse hippocampus section

ICC

IHC-Fr

Background

The GDP/GTP-exchange factor collybistin is composed of a dbl homology domain (DH) and a pleckstrin homology domain (PH) connected by a linker sequence. Three splice variants with different C-terminal regions have been described, so far.
Collybistin is supposed to be involved in the clustering of gephyrin, a scaffolding protein linking glycine and GABA receptors to microtubuli.

1 ) Papadopoulos T et al., Front Cell Neurosci (2011)
2 ) Saiepour L et al., J. Biol. Chem. (2010)
3 ) Jedlicka P et al., Mol. Cell. Neurosci. (2009)
4 ) Harvey K et al., J. Neurosci. (2004)
5 ) Kins S et al., Nat. Neurosci. (2000)

Protocols

ICC

Fixed Cells

IHC-Fr

Standard Condition

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