Synaptic Systems - Ca2+ channel P/Q-type alpha-1A

Cat. No.: 152 211

Amount: 100 µg

Price: $420.00

Cat. No. 152 211	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 100 up to 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet IHC-Fr: 1 : 500 gallery
Clone	23F11
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1921 to 2212 from rat Ca2+ channel P/Q-type α-1A (Cav2.1) (UniProt Id: P54282)
Reactivity	Reacts with: rat (P54282), mouse (P97445). Other species not tested yet.
Remarks	WB: To avoid protein aggregation, do not heat samples for SDS-PAGE. Due to the large size of this protein, we recommend NuPAGE 3-8% Tris-Acetate gels for SDS-PAGE. Less sensitive than the polyclonal antibodies (152 103, 152 205). ICC: 2% formaldehyde/PFA fixation is recommended. IHC-Fr: The following fixatives are possible: methanol, acetone, methanol-acetone, 4% formaldehyde/PFA
Data sheet	152_211.pdf

Transient Confinement of CaV2.1 Ca2+-Channel Splice Variants Shapes Synaptic Short-Term Plasticity.
Heck J, Parutto P, Ciuraszkiewicz A, Bikbaev A, Freund R, Mitlöhner J, Alonso M, Fejtova A, Holcman D, Heine M
Neuron (2019) : . 152 211 WB; tested species: rat

Cat. No.: 152 211

Amount: 100 µg

Price: $420.00

ICC

IHC-Fr

Background

Protocols

Ca2+ channel P/Q-type alpha-1A antibody - 152 211