Tailor-made Antibodies
and Tools for Life Science

Abeta42 antibody - 218 721

Abeta peptides are major components of neuritic plaques in Alzheimer's disease
Mouse monoclonal purified IgG
Cat. No.: 218 721
Amount: 100 µg
Price: $420.00
Cat. No. 218 721 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (ECL detection) (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 100 up to 1 : 500 gallery  
IHC-P: 1 : 200 gallery  
ELISA: yes ; suitable as capture and detector antibody (see remarks) gallery  
Clone 295F2
Subtype IgG2a (κ light chain)
Immunogen Synthetic peptide corresponding to AA 37 to 42 from human Abeta42 (UniProt Id: P05067)
Reactivity Reacts with: human (P05067), rat (P08592), mouse (P12023).
Other species not tested yet.
Specificity Specific for Abeta 42
Remarks

WB: Due to the small size of this protein/peptide, we recommend a 16% Schaegger gel system.
Boil membrane in PBS after blotting for 3min.
IHC: Antigen retrieval with formic acid is required.
IHC-P: Antigen retrieval with citrate buffer pH 6, followed by formic acid treatment, is required.
ELISA: Suitable as capture antibody for sandwich-ELISA. Please refer to the protocol for suitable detector antibodies.
Biotinylated mouse anti-Abeta38/40/42/43 antibody (cat. no. 218 211BT) can be used as detector antibody.

Data sheet 218_721.pdf
Cat. No.: 218 721
Amount: 100 µg
Price: $420.00