Abeta 42 - 218 703
A major component of neuritic plaques in Alzheimer's disease
Polyclonal rabbit purified antibody
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|Cat. No. 218 703||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 (ECL detection) (see remarks) gallery
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 100 up to 1 : 500 (see remarks) gallery
IHC-P/FFPE: 1 : 100 (see remarks) gallery
ELISA: yes suitable only as capture antibody, cat. no. 218 211 is recommended detector antibody for gallery (protocol)
|Immunogen||Synthetic peptide corresponding to AA 37 to 42 from human Abeta42 (UniProt Id: P05067)|
Reacts with: human (P05067), rat (P08592), mouse (P12023).
Other species not tested yet.
|Specificity||Specific for Abeta 42 with weak cross-reactivity to Abeta 40 in westernblots that is not apparent in ELISA tests.|
WB: Detects purified Abeta 42. Complex samples like brain extracts still have to be tested. Nitrocellulose membrane is recommended for blotting. Boil membrane after blotting for 3min.
IHC: Formic acid treatment required recommended protocol.
IHC-P: Formic acid treatment required.