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SY SY - Synaptic Systems SY SY - Synaptic Systems

Abeta 42 - 218 703

A major component of neuritic plaques in Alzheimer's disease

Polyclonal rabbit purified antibody
370.00 USD
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Cat. No. 218 703 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (ECL detection) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 100 up to 1 : 500 (see remarks) gallery  
IHC-P/FFPE: 1 : 100 (see remarks) gallery  
ELISA: yes suitable only as capture antibody, cat. no. 218 211 is recommended detector antibody for gallery   (protocol)
Immunogen Synthetic peptide corresponding to AA 37 to 42 from human Abeta42 (UniProt Id: P05067)
Reactivity Reacts with: human (P05067), rat (P08592), mouse (P12023).
Other species not tested yet.
Specificity Specific for Abeta 42 with weak cross-reactivity to Abeta 40 in westernblots that is not apparent in ELISA tests.
Remarks

WB: Detects purified Abeta 42. Complex samples like brain extracts still have to be tested. Nitrocellulose membrane is recommended for blotting. Boil membrane after blotting for 3min.

IHC: Formic acid treatment required recommended protocol.

IHC-P: Formic acid treatment required.

Datasheet 218_703.pdf


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References for Abeta 42 - 218 703

Neprilysin deficiency alters the neuropathological and behavioral phenotype in the 5XFAD mouse model of Alzheimer's disease.
Hüttenrauch M, Baches S, Gerth J, Bayer TA, Weggen S, Wirths O
Journal of Alzheimer's disease : JAD (2015) 444: 1291-302. 218 703 IHC; tested species: mouse
Abundance of Aβ₅-x like immunoreactivity in transgenic 5XFAD, APP/PS1KI and 3xTG mice, sporadic and familial Alzheimer's disease.
Guzmán EA, Bouter Y, Richard BC, Lannfelt L, Ingelsson M, Paetau A, Verkkoniemi-Ahola A, Wirths O, Bayer TA
Molecular neurodegeneration (2014) 9: 13. 218 703 IHC; tested species: human
Aβ38 in the brains of patients with sporadic and familial Alzheimer's disease and transgenic mouse models.
Reinert J, Martens H, Huettenrauch M, Kolbow T, Lannfelt L, Ingelsson M, Paetau A, Verkkoniemi-Ahola A, Bayer TA, Wirths O
Journal of Alzheimer's disease : JAD (2014) 394: 871-81. 218 703 IHC; tested species: human
N-truncated amyloid β (Aβ) 4-42 forms stable aggregates and induces acute and long-lasting behavioral deficits.
Bouter Y, Dietrich K, Wittnam JL, Rezaei-Ghaleh N, Pillot T, Papot-Couturier S, Lefebvre T, Sprenger F, Wirths O, Zweckstetter M, Bayer TA, et al.
Acta neuropathologica (2013) 1262: 189-205. 218 703 IHC; tested species: human
Axonal degeneration in an Alzheimer mouse model is PS1 gene dose dependent and linked to intraneuronal Aβ accumulation.
Christensen DZ, Huettenrauch M, Mitkovski M, Pradier L, Wirths O
Frontiers in aging neuroscience (2014) 6: 139. 218 703 IHC-P
Human Striatal Dopaminergic and Regional Serotonergic Synaptic Degeneration with Lewy Body Disease and Inheritance of APOE ε4.
Postupna N, Latimer CS, Larson EB, Sherfield E, Paladin J, Shively CA, Jorgensen MJ, Andrews RN, Kaplan JR, Crane PK, Montine KS, et al.
The American journal of pathology (2017) 1874: 884-895. 218 703 FACS; tested species: human
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Synaptic Systems GmbH | Rudolf-Wissell-Str. 28 | 37079 Göttingen | Germany
Phone: +49 (0)551/505 56-0 | Internet: http://www.sysy.com | E-Mail: sales@sysy.com