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GFAP sdAb - FluoTag-X2 - N3802-At488-L

GFAP is an astrocyte-specific type-III intermediate filament protein

This product was developed by   

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N3802-At488-L
Amount: 200 µl
Price: $485.00
Cat. No. N3802-At488-L 200 µl purified antibody, lyophilized from PBS, fluorescence-labeled with ATTO® 488

For many of the fluorescence labeled antibodies conjugated to ATTO® dyes from ATTO-TEC the established CyDye fluorescence detection systems can be used.
ATTO® 550 has similar exitation and emission properties as Cy3, and ATTO® 647N as Cy5.
ATTO®647N and ATTO®594 dyes are suitable for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy.
ATTO®594 is also recommended for PALM and dSTORM high resolution microscopy.

ATTO is a trademarks of ATTO-TEC GmbH, Siegen/Germany.
CyDyes, Cy3, and Cy5 are trademarks of GE-Healthcare Ltd.

.Azide was added before lyophilization. For reconstitution add 200 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Storage Shelf-Life: 3 month at -20°C, 12 moth at -80°C or below
Applications
 
WB: not recommended
IP: N/A
ICC: 1 : 500 gallery  
IHC: 1 : 500
IHC-P: not tested yet
Label ATTO 488, two fluorophores coupled to one FluoTag
Clone 1D12
Subtype single domain
Immunogen Recombinant protein corresponding to AA 1 to 432 from human GFAP (UniProt Id: P14136)
Reactivity Reacts with: human (P14136), rat (P47819), mouse (P03995).
Other species not tested yet.
Specificity Specific for GFAP, detects all isoforms.
Matching control protein/peptide 173-0P
Documents data sheet

Cat. No.: N3802-At488-L
Amount: 200 µl
Price: $485.00
Background

Glial fibrillary acidic protein GFAP is a glial-specific member of the intermediate filament protein family. This group comprises celltype-specific filamentous proteins with similar structure and function as scaffold for cytoskeleton assembly and maintenance.
Frequently, neural stem cells also express GFAP. In addition many types of brain tumors, probably derived from astrocytic cells, heavily express GFAP. This protein is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and others.
Point-mutations in the GFAP gene have been correlated to Alexander disease a fatal leukoencephalopathy that leads to the dysmyelination or demyelination of the central nervous system.

 

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.


In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two  fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.