Cat. No.: N3605-DBCO
Amount: 250 µg
Price:
$770.00
|
This product was developed by |
 |
Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
|
|
| Cat. No. N3605-DBCO |
250 µg purified antibody, lyophilized from PBS, fluorescence-labeled with DBCO.
Dibenzocyclooctyne (DBCO, ADIBO) is one of the most reactive cycloalkynes for strain promoted alkyne azide cycloaddition (spAAC), a copper-free click chemistry reaction. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications |
|
| Label | DBCO |
| Clone | 2A7 |
| Subtype | single domain |
| Immunogen | Recombinant protein corresponding to AA 1 to 419 from SARS-Cov-2 Nucleocapsid protein (UniProt Id: P0DTC9) |
| Specificity | Specific for nucleocapsid from SARS-CoV1 and SARS-CoV2 |
| Matching control protein/peptide | P0101 |
| Remarks |
This single domain antibody carries a C-terminal Biotin. |
| Data sheet | n3605-dbco.pdf |
|
|
The severe acute respiratory syndrome coronavirus type 2 (SARS-CoV2) is an enveloped positive-sense single stranded RNA virus that has been identified in the beginning of 2020 (1). It infects human host cells by docking via its spike protein (S) to the ACE2 surface receptor (2) and can cause mild to very severe and even deadly Covid-19 courses (3).
The very abundant Nucleocapsid or N-protein packages the viral RNA (4) and shares only little homology to other abundant members of the coronavirus family like NL63, 229E, HKU1 or OC43. This characteristic makes it a suitable target to discriminate between Covid-19 and other Coronavirus infections.
Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
In FluoTag®-Q each fluorophore is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a coupling efficiency of > 95% guarantees a highly linear relation between the number of target molecules and the intensity of fluorescence. This enables a direct count of the target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.