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mRuby sdAb - FluoTag-X2 - N3302-At488-L

mRuby is a bright monomeric red fluorescent protein

This product was developed by   

NanoTag-Biotechnologies

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaka heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N3302-At488-L
Amount: 200 µl
Price: $485.00
Cat. No. N3302-At488-L

2.5 µM fluorescently labeled sdAb in buffered saline, 50% glycerol, 0.09% sodium azide

Storage –80°C for up to 12 month
Applications WB: not recommended
IP: N/A
ICC: 1 : 500
IHC: not tested yet
IHC-P/FFPE: not tested yet
Label ATTO 488, two fluorophores coupled to one FluoTag
Clone 1F8
Immunogen Recombinant protein corresponding to AA 1 to 227 from Entacmaea quadricolor mRubymRUBY3
Specificity Specific for mRUBY3 and mRUBY derivatives
Documents data sheet

Cat. No.: N3302-At488-L
Quantity: 200 µl
Price: $485.00
Background

 mRuby and mRuby derivatives are bright monomeric red fluorescent proteins derived from Entacmaea quadricolar. They show exceptional resistance to denaturation at pH extremes.

 

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.


In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two  fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.

Protocols