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Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaka heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
|Cat. No. N3302-AF647-L||
2.5 µM fluorescently labeled sdAb in buffered saline, 50% glycerol, 0.09% sodium azide
|Storage||–80°C for up to 12 month|
WB: not recommended
ICC: 1 : 500
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Label||Alexa 647, two fluorophores coupled to one FluoTag|
|Immunogen||Recombinant protein corresponding to AA 1 to 227 from Entacmaea quadricolor mRubymRUBY3|
|Specificity||Specific for mRUBY3 and mRUBY derivatives|
mRuby and mRuby derivatives are bright monomeric red fluorescent proteins derived from Entacmaea quadricolar. They show exceptional resistance to denaturation at pH extremes.
Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.
In FluoTag®-X2 two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets two fluorophores to the protein of interest, which ensures up to two-fold („2X“)-brighter signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.