Synaptic Systems - ALFA Halo fusion sdAb

ALFA Halo fusion sdAb - N1541

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The ALFA-tag is a novel, rationally designed epitope tag (SRLEEELRRRLTE) that forms a small and stable α-helix that is functional irrespective of its position on the target protein in prokaryotic and eukaryotic hosts. Different ALFA-tag based products are available.

ALFA Selectors are based on ALFA-tag specific nanobodies covalently immobilized on 4% cross-linked agarose or magnetic agarose beads.

Camelid single domain antibody

Background

The sdAb anti-ALFA Halo fusion combines an alpaca-derived single-domain antibody (sdAb) targeting the ALFA-tag with a dehalogenase-based technology. The Halo protein tag sequence is fused to the C-terminus of the sdAb anti-ALFA. The final application of the sdAb anti-ALFA Halo fusion depends on the Halo ligand used. For instance, when combined with a fluorescent Halo ligand, our sdAb anti-ALFA Halo fusion can be used in immunofluorescence for detailed protein visualization and manipulation.

The ALFA system is NanoTag’s patented technology, consisting of the ALFA-tag – a rationally designed epitope tag (SRLEEELRRRLTE) – and a set of high-affinity anti-ALFA sdAbs, available in various formats to suit diverse application needs.

Halo refers to a modified bacterial enzyme, haloalkane dehalogenase, engineered to covalently bind synthetic ligands. These ligands can be conjugated to various functional groups such as fluorophores, biotin, or beads, enabling numerous biochemical, proteomic, and imaging applications.

Cat. No. N1541	50 µg purified antibody, lyophilized from PBS. Albumin was added for stabilization. For reconstitution refer to the NanoTag reconstitution and storage instructions. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	ICC: 1 : 500 IHC: 1 : 500 Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.
Label	Halo fusion
Clone	1G5
Specificity	ALFA-tag