CD62L sdAb - FluoTag-X2 - N1402-AF647-L

CD62L is a calcium dependent lectin

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FluoTags are camelid single domain antibodies consisting only of one antigen binding site of a Alpaka heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N1402-AF647-L

Amount: 200 µl

Price: $540.00

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Cat. No. N1402-AF647-L	200µl 5µM camelid sdAb in buffered saline, 50% glycerol, 0.09% sodium azide
Applications	WB: not tested yet IP: N/A ICC: 1 : 500 IHC: not tested yet IHC-P (FFPE): not tested yet FACS: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.
Label	Alexa 647, two fluorophores coupled to one FluoTag
Clone	P3C11
Subtype	single domain
Immunogen	Recombinant protein corresponding to AA 39 to 332 from human CD62L (UniProt Id: P14151)
Reactivity	Reacts with: human (P14151). Other species not tested yet.
Specificity	Specific for CD62L
Remarks	FACS: Use 1 µl for 1 x 10⁶ cells.

Cat. No.: N1402-AF647-L

Amount: 200 µl

Price: $540.00

Background

CD62L, also known as L-selectin, is a calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. It mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes and promotes initial tethering and rolling of leukocytes in endothelia.

1 ) Wedepohl S et al., Chembiochem (2017)
2 ) Mehta-D'souza P et al., J. Biol. Chem. (2017)
3 ) Spertini O et al., J. Immunol. (1991)
4 ) Huang K et al., J. Clin. Invest. (1991)
5 ) Kansas GS et al., APMIS (1992)
6 ) Bernimoulin MP et al., J. Biol. Chem. (2003)

Protocols

ICC

NanoTag IF/ICC

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