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GFP sdAb - FluoTag-X4 - N0304-At488-L

GFP is a widely used green fluorescent protein tag

 

This product was developed by   

NanoTag-Biotechnologies

Camelid single domain antibodies (sdAbs) consist only of one antigen binding site of an Alpaca heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Camelid single domain antibody

Cat. No.: N0304-At488-L
Amount: 200 µl
Price: $485.00
Cat. No. N0304-At488-L 200 µl specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with ATTO® 488

For many of the fluorescence labeled antibodies conjugated to ATTO® dyes from ATTO-TEC the established CyDye fluorescence detection systems can be used.
ATTO® 550 has similar exitation and emission properties as Cy3, and ATTO® 647N as Cy5.
ATTO®647N and ATTO®594 dyes are suitable for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy.
ATTO®594 is also recommended for PALM and dSTORM high resolution microscopy.

ATTO is a trademarks of ATTO-TEC GmbH, Siegen/Germany.
CyDyes, Cy3, and Cy5 are trademarks of GE-Healthcare Ltd.

.For reconstitution add 200 µl H2O. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Storage Up to three months: -20°C
Up to 12 months: -80°C or below
Protect form light!
Applications
 
WB: not recommended
IP: N/A
ICC: 1 : 250 gallery  
IHC: not tested yet
IHC-P: not tested yet
Label ATTO 488, two fluorophores coupled to two FluoTags each
Clone 1H1-1B2
Subtype single domain
Immunogen Recombinant protein corresponding to AA 1 to 238 from jellyfish GFP (UniProt Id: P42212)
Specificity Recognizes GFP, mEGFP, superfolder GFP, most common CFP and YFP variants.
Does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
Documents data sheet

References for GFP sdAb - N0304-At488-L

Caldendrin and myosin V regulate synaptic spine apparatus localization via ER stabilization in dendritic spines.
Konietzny A, Grendel J, Kadek A, Bucher M, Han Y, Hertrich N, Dekkers DHW, Demmers JAA, Grünewald K, Uetrecht C, Mikhaylova M, et al.
The EMBO journal (2022) 414: e106523. N0304-At488-L ICC; tested species: mouse,rat

Cat. No.: N0304-At488-L
Amount: 200 µl
Price: $485.00
Caldendrin and myosin V regulate synaptic spine apparatus localization via ER stabilization in dendritic spines.
Konietzny A, Grendel J, Kadek A, Bucher M, Han Y, Hertrich N, Dekkers DHW, Demmers JAA, Grünewald K, Uetrecht C, Mikhaylova M, et al.
The EMBO journal (2022) 414: e106523. N0304-At488-L ICC; tested species: mouse,rat
Background

Green fluorescent protein GFP and its derivates have become very popular and universal tools in cell biology. It is a monomeric and fast maturating protein with high photostability. Due to its sensitivity to pH changes it can be used as a biological pH indicator.

 

Unlabeled variants and several modifications of sdAbs like biotin, fluorophore or DBCO conjugation are available.

 

In FluoTag®-Q each fluorophore is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a coupling efficiency of > 95% guarantees a highly linear relation between the number of target molecules and the intensity of fluorescence. This enables a direct count of the target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

In FluoTag®-X two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Therefore, the reagent simultaneously targets up to four fluorophores (in X4 variants) to the protein of interest, which ensures extra-bright signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is ~ 3 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.

Protocols