Tailor-made Antibodies
and Tools for Life Science
Home|||||Technical Support

Chil3 / YM1 antibody mouse specific - HS-442 017

Chil3/YM1 is a marker protein for murine macrophages
Rat monoclonal purified IgG
Cat. No.: HS-442 017
Amount: 100 µg
Price: $415.00
Cat. No. HS-442 017 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 100 up to 1 : 250 gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: 1 : 1000 gallery  
Clone 189C10E2
Subtype IgG2b (λ light chain)
Immunogen Synthetic peptide corresponding to residues surrounding AA 280 of mouse Chil3 (UniProt Id: O35744)
Reactivity Reacts with: mouse (O35744).
No signal: human, rat.
Other species not tested yet.
Specificity Specific for mouse Chil3 / YM-1; no cross-reactivity with Chil4 / YM-2
Remarks

IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) may improve signal intensity in immunohistochemical staining.

Data sheet hs-442_017.pdf
Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.
Cat. No.: HS-442 017
Amount: 100 µg
Price: $415.00
Background

Chitinase-3-like protein 3, also referred to as  Chil 3, Ym1 and ECF-L, is a secreted protein that is primarily produced by macrophages during inflammation. It is only expressed in rodents, but expression profile and domain organization suggest a functional overlap with human AMCase and SI-CLP. Chil 3 has chemotactic activity for eosinophils, T-cells and polymorphonuclear leukocytes, and plays a role in the inflammatory response elicited by parasitic infections, lung disorders and allergy.

This lectin binds chitin, heparin and GlcN oligomers. Although Chil 3 shares homology with chitinase family proteins, it has no enzymatic activity.