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CD11b antibody - HS-384 117

CD11b is a component of the macrophage-1 antigen (Mac-1)
Rat monoclonal purified IgG
Cat. No.: HS-384 117
Amount: 200 µl
Price: $415.00
Cat. No. HS-384 117 200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: 1 : 100 up to 1 : 200 gallery  
IHC-Fr: 1 : 500 (see remarks) gallery  
Clone 298G2H5
Subtype IgG2a (κ light chain)
Epitop Epitop: AA 999 to 1016 from mouse CD11b (UniProt Id: P05555)
Reactivity Reacts with: mouse (P05555).
No signal: human (P11215).
Other species not tested yet.
Specificity Specific for CD 11b
Remarks

IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical stainings. Antibody incubation over-night at room temperature is recommended.
IHC-Fr: PFA fixation is recommended.

Data sheet hs-384_117.pdf
Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.

References for CD11b - HS-384 117

Influenza A Virus (H1N1) Infection Induces Microglial Activation and Temporal Dysbalance in Glutamatergic Synaptic Transmission.
Düsedau HP, Steffen J, Figueiredo CA, Boehme JD, Schultz K, Erck C, Korte M, Faber-Zuschratter H, Smalla KH, Dieterich D, Kröger A, et al.
mBio (2021) 125: e0177621. HS-384 117 IHC-P; tested species: mouse
Cat. No.: HS-384 117
Amount: 200 µl
Price: $415.00
Influenza A Virus (H1N1) Infection Induces Microglial Activation and Temporal Dysbalance in Glutamatergic Synaptic Transmission.
Düsedau HP, Steffen J, Figueiredo CA, Boehme JD, Schultz K, Erck C, Korte M, Faber-Zuschratter H, Smalla KH, Dieterich D, Kröger A, et al.
mBio (2021) 125: e0177621. HS-384 117 IHC-P; tested species: mouse
Background

CD 11b also called integrin alpha-M (ITGAM) is one protein subunit that forms together with CD18 the heterodimeric integrin αMβ2 complex, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3) (1). αMβ2 is expressed on polymorphonuclear neutrophils (PMN), monocytes, macrophages, some subsets of cytotoxic T lymphocytes, and NK cells.  Antibodies against CD 11b are frequently used to identify macrophages and microglia, however not all tissue-resident macrophages are CD11b positive. In the murine liver CD11b expression is rare and almost exclusively found on F4/80 negative cells (2). In the murine spleen CD11b+ cells are less numerous than F4/80+ cells, co-expression of CD11b and F4/80 is also rare and CD11b+ cells tend to be closer to the marginal zone (2). CD11b upregulation on residential alveolar macrophages is a marker of acute and chronic lung inflammation in mice (3). Also, in the brain CD11b is markedly increased during microglial activation (4).

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